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Bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of bacterial strain

A technology of putrefying bacteria and inorganic phosphorus, applied in the field of microorganisms, can solve problems such as environmental pollution and difficult control of chemical pesticides, and achieve the effect of efficient antagonism

Active Publication Date: 2016-09-21
BEIJING FORESTRY UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] The antagonistic bacteria strain Pseudomonas chlororaphisXZL-8 obtained by separating and screening from poplar rhizosphere soil in the present invention can decompose insoluble inorganic phosphorus, has high-efficiency antagonistic effect on poplar rot pathogen Cytosporachrysosperma, and can also produce volatile The antibacterial substance HCN can inhibit pathogenic bacteria and induce plant resistance; at the same time, it can also produce protease and esterase, degrade the cell wall of pathogenic fungi, inhibit or kill pathogenic bacteria; it is a bacterial strain that dissolves phosphorus and resists diseases. The strain of antagonistic bacteria Pseudomonaschlororaphis XZL-8 solves the current problems of difficult chemical pesticide control of poplar rot bacteria and serious environmental pollution

Method used

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  • Bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of bacterial strain
  • Bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of bacterial strain
  • Bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of bacterial strain

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Embodiment 1

[0082] Embodiment 1 Isolation, screening and identification of Pseudomonas chlororaphis (Pseudomonas chlororaphis) XZL-8 of the present invention

[0083] 1. Isolation and screening of Pseudomonas chlororaphis XZL-8

[0084] The bacterial strain of the present invention is collected from poplar rhizosphere soil on the campus of Beijing Forestry University in Haidian District, Beijing.

[0085] 1A) Take 5 g of poplar rhizosphere soil, put it into a sterilized Erlenmeyer flask equipped with 45 mL of sterile water, put it on a shaker, vibrate at 180 rpm for 30 min at 28 ° C, and let it stand to obtain a bacterial suspension (that is, 10 -1 Concentration of soil dilution); then take 1mL bacterial suspension and use 10-fold serial dilution method to dilute successively to obtain 10 -3 、10 -4 、10 -5 Concentration gradient dilution, take 0.1mL from each concentration gradient suspension, and spread it on LB medium (each 1000mL contains tryptone 10g, yeast extract 5g, NaCl 10g, aga...

Embodiment 2

[0107] Example 2 Exploration of Biological Characteristics of Pseudomonas chlororaphis XZL-8

[0108] 1A) Pick an activated Pseudomonas chlororaphis (Pseudomonas chlororaphis) XZL-8 strain from a bacterial loop (inoculation loop), and inoculate it in 100 mL of KMB liquid medium (each 1000 mL contains 20 g of peptone, 10 mL of glycerol, K 2 HPO 4 1.5g, MgSO 4 0.736g, pH 7.2-7.4) in a 250mL Erlenmeyer flask, then placed on a shaker, at 30 ° C, 200rpm shaking culture for 1d, to prepare the seed liquid of Pseudomonas chlororaphis XZL-8 strain, wherein, the seed liquid of Pseudomonas chlororaphis XZL-8 strain OD 600 The value is about 1.9;

[0109] 1B) according to the inoculum size of 1% (V / V), Pseudomonas chlororaphis XZL-8 bacterial strain seed liquid is inoculated in the 100mL Erlenmeyer flask that the NB liquid culture medium of the different salt (NaCl) concentration, pH value of 40mL is housed, at 30 After shaking culture at 200rpm for 2 days, inoculate 0.4mL of Pseudo...

Embodiment 3

[0114] Example 3 Determination of Pseudomonas chlororaphis (Pseudomonas chlororaphis) XZL-8 strain of the present invention's ability to degrade insoluble inorganic phosphorus

[0115] Pick a ring of activated Pseudomonas chlororaphis (Pseudomonas chlororaphis) XZL-8, and spot it in NBRIP medium (each 1000mL contains 10g of glucose, Ca 3 (PO 4 ) 2 5g, MgCl 2 ·6H 2 O5g MgSO 4 ·7H 2 O 0.25g, KCl 0.2g, (NH 4 ) 2 SO 40.1g, agar 15g, pH 7.0) in the center of the plate, and then place the plate in a constant temperature incubator at 30°C for upside-down culture, and continuously observe the insoluble inorganic phosphorus (Ca 3 (PO 4 ) 2 ) is decomposed to form a transparent circle on the plate. The appearance of a transparent circle indicates that the strain can degrade insoluble inorganic phosphorus (Ca 3 (PO 4 ) 2 ), if no transparent circle appears, it means that the strain cannot degrade insoluble inorganic phosphorus.

[0116] The measurement results showed that...

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Abstract

The invention discloses a bacterial strain with effects of degrading inorganic phosphorus and antagonizing cytospora chrysosperma and application of the bacterial strain. The strain provided by the invention is pseudomonas chlororaphis XZL-8 and has the microbial preservation number of CGMCC NO.12555, wherein the pseudomonas chlororaphis XZL-8 is isolated from the rhizosphere soil of poplar, can decompose the insoluble inorganic phosphorus into rapid available phosphorus which can be absorbed and utilized by plants, can produce HCN, protease and esterase, and can prevent cytospora chrysosperma caused by cytospora chrysosperma; bacteriostatic volatiles produced by the pseudomonas aeruginosa can also inhibit the growth of cytospora chrysosperma. The pseudomonas chlororaphis XZL-8 is a biological control potential strain with capability to degrade phosphorus, excellent antagonistic effect, high control effect and good environmental safety, and has good prospects of development and application.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a bacterial strain capable of decomposing insoluble inorganic phosphorus into available phosphorus that can be absorbed and utilized by plants, and capable of preventing and treating poplar rot bacteria and its application, which belongs to the field of microorganisms and can be used for plant protection , Prevention and treatment of plant diseases. Background technique [0002] Poplar is an important tree species for afforestation, ecological environment construction and social and economic development in my country. It has various varieties, strong resistance and adaptability. effect. Poplar is also the timber tree species with the largest cultivated area in my country, and it is also widely distributed all over the world. [0003] In recent years, poplar diseases have occurred frequently in China, with an average annual occurrence area of ​​more than 6.6 million mu, causing huge economic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/52C12N9/16A01N63/02A01N63/00A01N59/24A01P3/00A01P21/00C12R1/38
CPCA01N59/24A01N63/00A01N63/10C12N9/16C12N9/52C12N1/205C12R2001/38
Inventor 田呈明黄华毅于地美游崇娟梁英梅
Owner BEIJING FORESTRY UNIVERSITY
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