Hyaluronic acid fermentation liquor extraction method

A hyaluronic acid and extraction method technology, which is applied in the field of hyaluronic acid fermentation liquid extraction, can solve the problems of difficult hyaluronic acid extraction and separation, the yield cannot reach the enterprise, and the product purity is not high, so as to achieve stable feed liquid quality, The effect of low cost and increased economic benefits

Active Publication Date: 2016-09-21
SHANDONG FUFENG FERMENTATION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the high viscosity fermentation, extraction and separation of hyaluronic acid is difficult and the product purity is not high, the extraction and separation technology needs to be optimized and reformed, and an environmentally friendly process with low cost, low pollution, and easy industrial downstream separation and purification is required
Patented technology CN103342760A uses polyethylene glycol-sodium malate system to extract hyaluronic acid. The extraction rate of this technology can reach about 80%, but the method is complicated and difficult to operate, and the yield still cannot meet the requirements of the enterprise

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for extracting hyaluronic acid fermentation broth, comprising the steps of:

[0028] Step 1) After the hyaluronic acid fermentation is completed, adjust the pH of the hyaluronic acid fermentation broth to 4.0-6.5 with trichloroacetic acid, and then remove bacterial protein, macromolecular organic matter, colloids, particles, and suspended matter through microfiltration and ultrafiltration etc. to obtain hyaluronic acid filtrate A;

[0029] Step 2) Decolorize the hyaluronic acid filtrate A obtained in step 1), decolorize the hyaluronic acid filtrate A with modified cellulose, filter and collect the hyaluronic acid filtrate B, then adjust the pH of the hyaluronic acid filtrate B to 6.0-6.5, and then add 2 times 95% ethanol by volume to precipitate the crude extract of hyaluronic acid;

[0030] Step 3) Dissolve the crude hyaluronic acid extract with the same volume of 0.1mol / L sodium acetate aqueous solution as in step 1) hyaluronic acid fermentation broth, and a...

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Abstract

The invention belongs to the technical field of microbial polysaccharide production and discloses a hyaluronic acid fermentation liquor extraction method. The method includes the first step of obtaining hyaluronic acid filter liquor A, the second step of conducting decoloring, impurity removal and precipitation, the third step of conducting dissolving and complexing, the fourth step of conducting dissociation and filtering, and the fifth step of carrying out precipitation, dewatering and drying. The extraction method has the advantages of being high in extraction yield and product purity, little in environment pollution and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial polysaccharide production, and in particular relates to a hyaluronic acid fermentation liquid extraction method. Background technique [0002] Hyaluronic acid (Hyaluronic Acid, HA), also known as hyaluronic acid, is a linear polymeric acidic mucopolysaccharide widely present in the connective tissue of organisms. Hyaluronic acid is composed of (1∼3)-2-acetylamino-2-deoxy-β-glucose (1∼4)-O-β-D-glucuronic acid disaccharide repeating units. [0003] Hyaluronic acid has functions such as moisturizing, water retention, lubricity, water absorption, biocompatibility, biodegradability, cell fluidity and cell attachment, and its aqueous solution has greater viscosity and rheology. Therefore, it has high application value in cosmetics, plastic surgery, medicine, clinical medicine, health food and other fields. With the maturity of hyaluronic acid research and utilization technology in the international ...

Claims

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Application Information

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IPC IPC(8): C08B37/08
CPCC08B37/0072
Inventor 贾召鹏赵兰坤冯珍泉刘元涛李树标徐田华刘超
Owner SHANDONG FUFENG FERMENTATION CO LTD
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