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Prawn feed containing rhodobacter capsulatus strain and application thereof

A technology of encapsulated rhodobacter and prawn feed, applied in the field of bioengineering, can solve problems such as frequent breeding diseases and deterioration of breeding water

Inactive Publication Date: 2016-09-21
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve many problems that the existing high-density aquaculture model easily leads to frequent aquaculture diseases and serious deterioration of aquaculture water, the present invention provides a bacterial strain with antibacterial and digestive enzyme activity and its screening method and application. A novel strain isolated from the intestinal tract of Penaeus vannamei with antimicrobial activity and protease, amylase and lipase production activities

Method used

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  • Prawn feed containing rhodobacter capsulatus strain and application thereof
  • Prawn feed containing rhodobacter capsulatus strain and application thereof
  • Prawn feed containing rhodobacter capsulatus strain and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0043] The Litopenaeus vannamei used in this example was taken from Xinyongfeng Prawn Farm in Hangu District, Tianjin;

[0044] The 2216E medium used in this example was prepared with a nine-salt solution;

[0045] The 2216E medium used in this example includes the weight ratio of the following components: peptone 5g / L, yeast extract 1g / L, iron phosphate 0.1g / L, agar 20g / L pH7.6-7.8.

[0046] The indicator bacterium described in this embodiment is Vibrio anguillarum ( Vibrio anguillarum ), purchased from the Institute of Microbiology, Chinese Academy of Sciences, and preserved in the Hydrobiology Laboratory of Tianjin Normal University.

[0047] In this embodiment Rhodobacter capsulatus Screening method for R3 strain:

[0048] Isolation and purification of bacterial strains: Under sterile conditions, take the whole intestine of prawns in a homogenizer, grind in an ice bath, and dilute the grinding solution with a gradient of 10-1 to 10-8 with a nine-salt solution; select ...

Embodiment 2

[0051] This embodiment has the activity of producing digestive enzymes Rhodobacter capsulatus The screening method of the R3 strain, and its separation and purification method are the same as in Example 1, and will not be repeated here.

[0052] The digestive enzyme activity test was carried out on the purified strain:

[0053] A. Transfer the purified strain to the liquid culture medium at 28°C for constant temperature shaking culture overnight, use sterile tweezers to pick up a piece of sterile filter paper and place it in the culture medium to soak the bacteria solution, and place it in the prepared skim milk medium At the corresponding position, culture at a constant temperature of 28°C for 24 hours, and screen the strains that can decompose proteins and produce transparent circles.

[0054] B. Transfer the purified strain to the liquid culture medium with constant temperature shaking at 28°C overnight, use sterile tweezers to pick up a piece of sterile filter paper and ...

Embodiment 3

[0060] The present invention obtained by choosing the above-mentioned embodiment has antibacterial activity and produces digestive enzyme activity Rhodobacter capsulatus R3 strain was identified and analyzed, the specific identification and analysis methods and corresponding identification results are as follows.

[0061] Adopt the method screening of above-mentioned embodiment to have antibacterial activity and produce digestive enzyme activity Rhodobacter capsulatus The R3 strain was inoculated on a 2216E medium plate, cultured upside down at 28°C for 24 hours, and the growth state of the colony was observed. For the strain morphology and physiological and biochemical tests, refer to the Industrial Microbiology Test Technical Manual (Zhuge Jian, Wang Zhengxiang, Industrial Microbial Test Technical Manual [M], China Light Industry Press, 1994) and Bergey's Bacteria Identification Manual (R.E Buchanan, N.E Gibbons, Bergey's Bacteria Identification Manual [M], Science Press...

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Abstract

The invention discloses prawn feed containing rhodobacter capsulatus strain. The prawn feed containing rhodobacter capsulatus strain is characterized by being prepared from prawn feed, rhodobacter capsulatus strain fermentation liquor and sodium alginate, wherein 10g of odium alginate and 20mL of rhodobacter capsulatus strain fermentation liquor are added into each 100g of prawn feed until concentration is 3-6*107CFU (Colony Forming Unit) / g; the rhodobacter capsulatus strain is a (i) Rhodobacter capsulatus( / i) R3 strain and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No:11753. The rhodobacter capsulatus is used as a feed additive to manufacture the prawn edible feed, a feeding result shows that the growth speed of the prawn which eats the feed in which the rhodobacter capsulatus is added is greatly improved compared with the prawn which eats common feed, and immunity is obviously improved. Since the rhodobacter capsulatus is used for manufacturing the feed, the production period is short, the production cost is low, a production condition can be easily controlled, the antimicrobial activity is high, the immunity can be obviously enhanced and the like, and the prawn feed has the potential of industrial production and has a good production application prospect.

Description

[0001] The present invention is funded by the 973 project "Immunological approaches and key technical principles for the prevention and treatment of prawn white spot syndrome", project number 2012CB114405, and Tianjin Normal University Biology Outstanding Young Teacher Funding Project, project number ZX110QN008. technical field [0002] The invention relates to a prawn feed containing capsulated rhodobacter strains and an application thereof, belonging to the technical field of bioengineering. Background technique [0003] Litopenaeus vannamei ( Litopenaeus vannamei ), also known as Penaeus vannamei ( White Prawn ), Litopenaeus vannamei is delicious in meat, and it is also known as the world's three largest shrimps together with Chinese prawns and Penaeus monodon, and it plays an extremely important role in my country's prawn farming industry. However, with the development of intensive culture of Litopenaeus vannamei, the outbreak of prawn diseases has caused serious harm ...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/18
Inventor 左志晗孙金生李艳红
Owner TIANJIN NORMAL UNIVERSITY
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