A device and method for measuring the influence of gaseous substances on the motility of nematodes

Abstract

The invention discloses a determination device for influence of gas phase substance on motion capability of nematodes. The device comprises a container (5). The side surface of the container is provided with volume scales (8). A transparent cover plate (9) is arranged on the container and provided with a vertical handle (10). A culture dish (3) is accommodated in the container (5) and internally provided with a nematode culture medium (4). The container (5) has an air inlet pipe (1) and an exhaust pipe (6). The air inlet pipe (1) is provided with an air inlet pipe cover (6) in the shape of a gooseneck and provided with an exhaust pipe cover (7). When the two pipe covers are closed, air can be prevented. The invention further discloses a determination method for influence of gas phase substance on motion capability of nematodes.By making nematodes in the culture dish (3) directly contact gas phase substance, the mode of action for gas phase substance is more suitable. Therefore, influence of gas phase substance on motion capability of nematodes can be conveniently observed.

Description

technical field [0001] The invention belongs to the application field of biotechnology, and in particular relates to a measuring device and a measuring method for the influence of gas phase substances such as smoke on nematode motility. Background technique [0002] The model organism Caenorhabditis elegans is the simplest model suitable for studying the nervous system of higher animals. Based on the "small" characteristics of nematodes and their similarities with humans in many aspects, many assessment tools including toxicological assessment of C. Reproductive ability, neurotoxicity, enzyme activity determination in vivo, etc. Changes in motion state include body bending frequency, head swing frequency, basic motion ability and motion rate, etc. [0003] Existing methods to determine the effect of substances on nematodes are usually directly added to the nematode culture medium to observe the influence of chemical substances on the motility of nematodes, but this method ...

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Problems solved by technology

[0003] Existing methods to determine the effect of substances on nematodes are usually directly added to the nematode culture medium to observe the influence of chemical substances on the motility of nematodes, but this method cannot measure gas samples. If the medium is added after gas capture, it cannot Observe the direct impact of gaseous substances on nematodes. Second, the exposure methods are quite different, which cannot truly reflect the reaction of nematodes to gaseous substances. The present invention can quickly and directly observe nematodes in different gaseous phases Changes in the movement ability in the presence of substances, and the concentration of the gas to be measured can be adjusted, and can be used for the detection of gas phase substances such as smoke and toxic gases

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