Tissue Culture and Rapid Breeding Method of Pig Bloodwood

A tissue culture, porcine blood wood technology, applied in the field of plant reproduction, can solve problems such as population difficulties and the reduction of wild resources of porcine blood wood, and achieve the effect of promoting division and differentiation, solving the problem of increasing reduction and small genetic variation.

Inactive Publication Date: 2018-07-03
红谷滩建设集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Still another object of the present invention is to provide a rapid breeding method for blood tree tissue culture, which uses tissue culture of the young shoots of blood wood in the same year to obtain seedlings of blood wood, and transplants the seedlings into the seedbed to continue culturing to obtain blood wood Transplanting seedlings, this breeding method can effectively improve the breeding coefficient of S. hemataceae, retain the excellent genetic characteristics of S. hemataceae, the survival rate of seedlings is as high as 90%, and the survival rate of transplanted seedlings is as high as 80%. After transplanting, the seedlings grow well , there is no phenomenon of "little old man seedlings", which solves the problem of the dwindling wild resources of the blood tree and the difficulty of natural regeneration of the population, and is conducive to the effective protection of the rare and endangered plant of the blood tree

Method used

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  • Tissue Culture and Rapid Breeding Method of Pig Bloodwood

Examples

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Effect test

Embodiment 1

[0051] A rapid propagation method of pig blood wood tissue culture, comprising the following steps:

[0052] A. Cut out the new shoots of the old-fashioned pig blood tree, take the 5cm branches at the top, soak the branches with a 2% aqueous solution of detergent solution for 20 minutes, take them out, rinse them with linear tap water for 15 minutes, and then put them into natural water. Soak in the disinfectant solution for 30min, finally the branch is rinsed 3 times with sterile water and removes its surface moisture with sterile filter paper to obtain the explant, wherein,

[0053] The preparation method of the natural disinfectant is as follows: 10 parts by weight of garlic, 7 parts by weight of lemongrass, 6 parts by weight of Daidai, 6 parts by weight of Melaleuca mellifera, 5 parts by weight of Eucalyptus blue and 3 parts by weight of cloves are crushed to 50 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux extraction at ...

Embodiment 2

[0076] A rapid propagation method of pig blood wood tissue culture, comprising the following steps:

[0077] A. Cut off the new shoots of the old-fashioned pig blood tree, take the 8cm branches at the top, soak the branches with a 2% aqueous solution of detergent for 20 minutes, take them out, rinse them with linear tap water for 18 minutes, and then put them into natural water. Soak in the disinfectant solution for 30min, finally the branch is rinsed 4 times with sterile water and removes its surface moisture with sterile filter paper to obtain the explant, wherein,

[0078] The preparation method of the natural disinfectant is: crush 11 parts by weight of garlic, 8 parts by weight of lemongrass, 7 parts by weight of Daidai, 7 parts by weight of Melaleuca mellifera, 6 parts by weight of Eucalyptus blue and 4 parts by weight of cloves to 80 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux at 60°C for 3 hours, filter, and the obt...

Embodiment 3

[0101] A rapid propagation method of pig blood wood tissue culture, comprising the following steps:

[0102] A. Cut out the new shoots of the hemogonia in the same year, take the 10cm branches at the top, soak the branches with a 2% aqueous solution of detergent for 20 minutes, take them out, rinse them with linear tap water for 20 minutes, and then put them into natural water. Soak in the disinfectant solution for 30min, finally the branch is rinsed 5 times with sterile water and removes its surface moisture with sterile filter paper to obtain the explant, wherein,

[0103] The preparation method of the natural disinfectant is as follows: 12 parts by weight of garlic, 9 parts by weight of lemongrass, 8 parts by weight of Daidai, 8 parts by weight of Melaleuca mellifera, 7 parts by weight of Eucalyptus blue and 5 parts by weight of cloves are crushed to 100 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux extraction at 60°C for ...

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Abstract

The invention provides a tissue culture and rapid propagation method of euryodendron excelsum. The method comprises the following steps: selecting and disinfecting explants, inducing clustered buds, proliferating clustered buds, rooting and culturing, culturing strong seedlings and culturing a seedling bed. Compared with the conventional seedling-raising method of euryodendron excelsum, the method does not adopt euryodendron excels seeds; the new sprout seedlings of the euryodendron excelsum are adopted; few plant raw materials are needed; the euryodendron excelsum is subjected to short-time and mass propagation; the heritable variation of the variety is low; the young seedlings is high in quality; the survival rate of the young seedlings reaches up to 90%; the survival rate of transplanted seedlings reaches up to 80%; after transplanting, the seedlings are excellent in growth vigor; the phenomenon of small seedling is avoided; the problems that the euryodendron excelsum wild resources are increasingly reduced and natural reproduction of the population is difficult can be effectively solved; the technical support is provided for effective protection of euryodendron excelsum serving as a rare and threatened plant.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a rapid propagation method of blood tree tissue culture. Background technique [0002] Euryodendron excelsum H.T.Chang belongs to the family Theaceae (Theaceae) Euryodendron excelsum is a perennial woody plant. It is a rare and endangered plant with a unique monotype in my country and has been listed as a national second-class protected plant. The systematology position of the pig blood wood is special, and it is an important material for studying the phylogeny and morphological evolution of the camellia family. At the same time, its trunk is straight, the heartwood is beautiful, and the wood is extremely hard. It is an excellent building material and furniture material, and has a certain economic value. . At present, the pig blood tree is only distributed in the area of ​​Bajia Town, Yangchun County, Guangdong Province, and only one population remains. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G9/14A01G9/24
CPCA01G9/20A01G24/00A01G24/23A01H4/00A01H4/001A01H4/008Y02A40/25
Inventor 陈思
Owner 红谷滩建设集团有限公司
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