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Hepatoma cell line STL-C1 derived from human hepatoma a-carcinoma tissue and establishment method thereof

A technology of STL-C1, liver cancer cell line, applied in the field of liver cancer cell line STL-C1 and its construction

Active Publication Date: 2016-08-17
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are still no reports on liver cancer cell lines and their construction methods derived from human liver cancer paracancerous tissues.

Method used

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  • Hepatoma cell line STL-C1 derived from human hepatoma a-carcinoma tissue and establishment method thereof
  • Hepatoma cell line STL-C1 derived from human hepatoma a-carcinoma tissue and establishment method thereof
  • Hepatoma cell line STL-C1 derived from human hepatoma a-carcinoma tissue and establishment method thereof

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Embodiment 1

[0040] 1. Experimental reagents and materials

[0041] 1. Experimental reagents

[0042] (1) Cell culture reagents: DMEM / F12 (1:1) culture medium (Gibco, USA), 10% (v / v) fetal bovine serum (Biochrom AG, USA); 100U / L penicillin and 0.1mg / L chain Mycin (Shanghai Boguang Biological Company) had a final working concentration of 100 U / mL and 0.1 mg / mL; human recombinant bFGF stem cell growth factor (Peprotech, USA); insulin-transferrin-selenium supplement (ITS Lipid Media Supplement, 100× ) (Sigma, USA), composed of 1.0mg / ml recombinant human insulin, 0.55mg / ml human transferrin and 0.5μg / ml sodium selenite, added to 10% (v / v) in the DMEM / F12 culture fluid of fetal calf serum;

[0043] (2) Other materials required for cell culture and cell separation: D-Hank's balanced salt solution (pH 7.2-7.4), collagenase type IV (Sigma, USA), heparin injection, 50 μm pore size cell nylon mesh (BD Biosciences, USA), Percoll solution, phosphate buffered saline (PBS) (pH=7.4), 0.25% (w / v) tryp...

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Abstract

The present invention relates to the field of microbial animal cells, and specifically a hepatoma cell line STL-C1 derived from human hepatoma para-carcinoma tissue. The cell line has a preservation number CCTCC No:C201630. The present invention also provides an establishment method and application of the cell line. The established hepatoma cell line STL-C1 is derived from human hepatocellular hepatoma para-carcinoma tissue, and can be cultured in vitro for a long term and has stable passage; and in vivo experiments show that the cell line still has good tumorigenic property, and is completely different from the currently existing established common hepatoma cell lines and even from the hepatoma cancer embolus cell line. The hepatoma cell line STL-C1 has important usage and reference value to the study of the formation of satellite lesions, and biological behaviors suahc as recurrence and transfer of primary hepatic carcinoma, is a novel cell model for basic and clinical research of hepatoma, and has broad application prospects.

Description

technical field [0001] The present invention relates to the field of microbial animal cells, specifically, a liver cancer cell line STL-C1 isolated and established from human hepatocellular carcinoma paracancerous tissue samples, its construction method and application. Background technique [0002] Primary liver cancer (Hepatocellular carcinoma, HCC) is one of the most common malignant tumors in my country, ranking second only to lung cancer in tumor-related deaths, and the prognosis of patients is extremely poor. Although surgical resection of the primary lesion is still the most effective treatment for liver cancer at present, most of the patients are in the middle and advanced stage and have metastases in and out of the liver when they are diagnosed. Therefore, the surgical resection rate is low, the recurrence rate is high, and the clinical effect is still not satisfactory. Therefore, it is particularly urgent and important to study the molecular mechanism of the occurr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N2500/25C12N2501/115C12N2509/00
Inventor 王红阳鄢和新王明达吴晗付恭博周徐
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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