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A strain of Xanthomonas nyw79 and its application

A technology of Xanthomonas and strains, applied in the field of biotechnology applications, can solve problems such as gene mutation and gene change, achieve the effect of increasing yield and solving the problem of decolorization

Active Publication Date: 2019-05-21
ORDOS ZHONGXUAN BIOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to its strong mutagenic effect, nitrosoguanidine can also induce concurrent mutations in adjacent genes, and it is especially easy to induce mutations in genes near the DNA replication fork. Some mutagenic strains may cause certain genetic changes or defects

Method used

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  • A strain of Xanthomonas nyw79 and its application
  • A strain of Xanthomonas nyw79 and its application
  • A strain of Xanthomonas nyw79 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0107] Embodiment 1: Use NYW79 bacterial strain to produce pigment-free xanthan gum

[0108] The implementation steps of this embodiment are as follows

[0109] A. Activation culture

[0110] The preparation method of the slant medium is as follows: 0.8 parts by weight of sucrose, 0.06 parts by weight of peptone, 0.10 parts by weight of yeast extract, 0.4 parts by weight of beef extract, 1 part by weight of agar, 0.2 parts by weight of K 2 HPO 4 ·3H 2 O, 0.08 parts by weight NaCl and 0.08 parts by weight MgSO 4 Dissolve in 100 parts by weight of water, stir evenly, adjust the pH value to 7.0 with 0.1 mol / L nitric acid or sodium hydroxide aqueous solution, and then sterilize at 121°C for 30 minutes to obtain the slant medium.

[0111] Pick a ring of slant-preserved Xanthomonas NYW79 strains and inoculate them into the slant medium of eggplant bottles, and cultivate them at a temperature of 30°C for 72 hours in the equipment sold by Shandong Weifang Jingying Medical Devices ...

Embodiment 2

[0121] Embodiment 2: Use NYW79 bacterial strain to produce pigment-free xanthan gum

[0122] The implementation steps of this embodiment are as follows

[0123] A. Activation culture

[0124] The preparation method of the slant medium is as follows: 1.0 parts by weight of sucrose, 0.10 parts by weight of peptone, 0.08 parts by weight of yeast extract, 0.5 parts by weight of beef extract, 3 parts by weight of agar, 0.1 parts by weight of K 2 HPO 4 ·3H 2 O, 0.10 parts by weight NaCl and 0.12 parts by weight MgSO 4 Dissolve in 100 parts by weight of water, stir evenly, adjust the pH value to 7.0 with 0.5 mol / L sulfuric acid or potassium hydroxide aqueous solution, and then sterilize at 121° C. for 30 minutes to obtain the slant medium.

[0125] Pick a ring of slant-preserved Xanthomonas NYW79 strains and inoculate them into the slant medium of eggplant bottles, and cultivate them at a temperature of 30°C for 72 hours in the equipment sold by Shandong Weifang Jingying Medical ...

Embodiment 3

[0135] Embodiment 3: Use NYW79 bacterial strain to produce pigment-free xanthan gum

[0136] The implementation steps of this embodiment are as follows

[0137] A. Activation culture

[0138] The preparation method of the slant medium is as follows: 1.2 parts by weight of sucrose, 0.08 parts by weight of peptone, 0.12 parts by weight of yeast extract, 0.6 parts by weight of beef extract, 2 parts by weight of agar, 0.3 parts by weight of K 2 HPO 4 ·3H 2 O, 0.12 parts by weight NaCl and 0.10 parts by weight MgSO 4 Dissolve in 100 parts by weight of water, stir evenly, adjust the pH value to 7.0 with 0.3 mol / L hydrochloric acid or sodium carbonate aqueous solution, and then sterilize at 121° C. for 30 minutes to obtain the slant medium.

[0139] Pick a ring of slant-preserved Xanthomonas NYW79 strains and inoculate them into the slant medium of eggplant bottles, and cultivate them at a temperature of 30°C for 72 hours in the equipment sold by Shandong Weifang Jingying Medical...

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Abstract

The invention relates to a Xanthomonas sp. NYW79 and its application. The steps of using the NYW79 bacterial strain to produce the non-pigmented xanthan gum include steps such as activation culture, liquid seed culture, fermentation culture and xanthan gum precipitation. The yield of xanthan gum produced by NYW79 strain is more than 14.9% higher than that of NY07 strain by mutagenesis; the OD value of fermentation broth is reduced by more than 77.1%. The production of xanthan gum by NYW79 strain can fundamentally solve the decolorization problem of xanthan gum products. At the same time, the yield of xanthan polysaccharide was also significantly increased.

Description

【Technical field】 [0001] The invention belongs to the application field of biotechnology. More specifically, the present invention relates to a strain of Xanthomonas sp. NYW79, and also relates to the use of Xanthomonas sp. NYW79. 【Background technique】 [0002] Xanthan gum (xanthan gum) is a kind of microbial extracellular heteropolysaccharide produced by fermentation of Xanthomonas campestris with carbohydrates as the main raw material. Its molecules are composed of D-glucose, D-mannose, D - Composed of glucuronic, acetic and pyruvic acids. Xanthan gum has many unique physical and chemical properties, mainly manifested in significant pseudoplasticity, good thickening property, good emulsification stability, high viscosity at low concentration, strong suspending ability for solids and wide temperature resistance , Freeze-thaw resistance, acid and alkali resistance, etc. It is widely used in the food industry as a thickener, emulsifier, quality improver, and suspending ag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P19/06C12R1/64
CPCC12P19/06C12N1/205C12R2001/64
Inventor 尹丽华王恒书
Owner ORDOS ZHONGXUAN BIOCHEM
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