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Alisma polysaccharide with effect of improving insulin resistance and its extraction method and application

An insulin resistance and extraction method technology, applied in the field of Alisma polysaccharide and its extraction, can solve the problems of difficulty in identifying, separating and purifying polysaccharides, and separating and purifying various substances, and achieves improved yield, obvious hypoglycemic function, and improved insulin. effect of resistance

Active Publication Date: 2021-10-22
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The extraction methods of polysaccharides mainly include heating extraction, enzymatic hydrolysis, ultrasonic extraction, etc. Each of these extraction methods has its own advantages and disadvantages. The composition of the existing Alisma polysaccharides is very complicated, and it is difficult to separate and purify various substances. Difficult, it is difficult to identify a single sugar that improves insulin resistance

Method used

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  • Alisma polysaccharide with effect of improving insulin resistance and its extraction method and application
  • Alisma polysaccharide with effect of improving insulin resistance and its extraction method and application
  • Alisma polysaccharide with effect of improving insulin resistance and its extraction method and application

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Embodiment 1

[0023] Take 40 mesh Alisma powder, with the mass volume ratio of material to liquid as 1:20, add cellulase with a pH of 4.5 and a mass concentration of 0.6% citric acid buffer to obtain a mixed solution, and heat the mixed solution in a 40-degree constant temperature water bath 2h, and then inactivated in boiling water for 10min to obtain the Alisma polysaccharide extract.

[0024] Take 100 mesh Alisma powder, with the mass volume ratio of material to liquid being 1:25, add cellulase mass volume concentration of pH 4 and 0.5% citric acid buffer to obtain a mixed solution, and place the mixed solution in a 40-degree constant temperature water bath Heated for 2 hours, and then inactivated in boiling water for 10 minutes to obtain the Alisma polysaccharide extract. Filter the obtained Alisma polysaccharide extract, collect the filtrate, centrifuge at 4000r / min for 15min, collect the supernatant, and concentrate the supernatant to 1 / 3 of its original volume to obtain a concentrate...

Embodiment 2

[0026] Example 2 Extraction and Analysis of Alisma polysaccharides with Improving Insulin Resistance in the Present Invention

[0027] (1) Alisma polysaccharide of the present invention and its extraction method

[0028] 1. Inspect the particle size of Alisma powder (180 mesh), solid-liquid ratio (1:10, 1:20-1:30, 1:30, 1:40g / mL), pH (4, 4.5, 5, 5.5, 6, 6.5), cellulase concentration (0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6%), enzymatic hydrolysis temperature (35, 40, 45, 50, 55, 60 degrees), enzymatic hydrolysis time (30, 60, 90, 120, 150, 180min) on the extraction rate of Alisma polysaccharide. Get the best process, and then use the response surface method to optimize the extraction process to get the best process conditions.

[0029] 2. Accurately weigh 2.000g of Alisma powder of 40-100 mesh, add cellulase concentration of pH 4.5 and 0.4% citric acid buffer solution with a solid-liquid ratio of 1:20 (g / mL); Heat it in a 40-degree constant-temperature water bath for 2 hours, and t...

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Abstract

The invention relates to the technical field of traditional Chinese medicine preparation, in particular to an alisma polysaccharide capable of improving insulin resistance, an extraction method and application thereof. The method for extracting Alisma polysaccharides with the effect of improving insulin resistance comprises the following steps: selecting pulverized dry material with a particle size of 40-100 mesh Alisma powder, extracting with cellulase, the enzyme concentration is 0.4%, and the enzymatic hydrolysis temperature is 35-100 mesh. 40 degrees, enzymatic hydrolysis time 120-180min, pH 4-4.5, mass-to-volume ratio of solid to liquid 1:20-1:30; heat up and inactivate to obtain Alisma polysaccharide extract. The beneficial effect of the present invention is that: the method for extracting Alisma polysaccharides with the function of improving insulin resistance of the present invention adopts an enzyme extraction method to extract Alisma polysaccharides, the process is simple, and the yield of Alisma polysaccharides is improved. The extraction rate can be as high as 18.89%. The Alisma polysaccharide obtained by the extraction method of the invention has obvious blood sugar-lowering function, and opens up a new way for the intensive processing of Alisma.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine preparation, in particular to an alisma polysaccharide capable of improving insulin resistance, an extraction method and application thereof. Background technique [0002] Insulin resistance (insulin resistance, IR) refers to the decreased sensitivity of the target organs of insulin action to insulin action, which is manifested as the impairment of glucose utilization by peripheral tissues, mainly muscle and adipose tissue. IR is not only the key link and main feature of type 2 diabetes mellitus (T2DM), but also closely related to the occurrence of other complications such as hypertension, atherosclerosis, metabolic syndrome, ovarian cysts, Alzheimer's disease, and tumors. Drugs currently used clinically to improve and treat insulin resistance, such as insulin sensitizers thiazolidinediones such as pioglitazone and rosiglitazone, have side effects such as edema and weight gain...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P5/50A61P3/10A61K31/715
CPCA61K31/715C08B37/00C08B37/0003
Inventor 吴水生许文黄小强张雪罗奋熔李小艳
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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