Micro-fluidic chip based on SERS detection, preparation method and application thereof
A microfluidic chip and substrate technology, applied in chemical instruments and methods, measuring devices, and laboratory containers, can solve the problems of large loss, complex structure, and uneven distribution of detected particles when liquid flows, and achieve The effect of increasing the sensitivity
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Embodiment 1
[0033] Embodiment 1 The preparation method of the microfluidic chip based on SERS detection according to the present invention comprises the following steps:
[0034] 1) Preparation of superhydrophobic titanium dioxide coating solution: 0.1 g of titanium dioxide powder (P 25 ) was mixed with 10mL of absolute ethanol, and then ultrasonicated at 100Hz for 30min to form a 0.01g / mL titanium dioxide suspension, then 0.2mL of octadecyltrimethoxysilane was added, and then ultrasonically mixed at 100Hz for 10min. React for 12 hours to obtain a superhydrophobic titanium dioxide coating solution; the volume ratio of the silane to the titanium dioxide suspension is 0.02:1;
[0035] 2) Preparation of a substrate with a superhydrophobic layer: use a spin coater to spin-coat the superhydrophobic titanium dioxide coating solution onto the cleaned upper surface of the substrate, and then place it in an oven at 100°C for 1 hour to obtain a substrate with a superhydrophobic layer , the contact...
Embodiment 2
[0043] Example 2 The microfluidic chip based on SERS detection prepared by the preparation method of the present invention includes a substrate 1 and a T-shaped flow channel 2 arranged on the surface of the substrate, and the two ends of the T-shaped flow channel 2 are As two flow channel inlets (21,22), the end of the vertical flow channel 23 of the T-shaped flow channel 2 is used as the detection area 231; Each region is evenly distributed with hydrophobic patterns 24, and each hydrophobic pattern 24 corresponds to the corresponding position of the substrate as a superhydrophobic region, and the part outside the hydrophobic pattern is a superhydrophilic region; The number of hydrophobic patterns in the area is decreasing, that is, the distance between adjacent hydrophobic patterns is gradually increasing, that is, the proportion of the corresponding hydrophilic area on the substrate 1 is gradually increasing; the end of the vertical channel 23 is provided with a detection are...
Embodiment 3
[0045] The application of the micro-control flow chip described in Example 3 in the simultaneous detection of liquid analytes is characterized in that: the application method is carried out according to the following steps:
[0046] (1) configuration of Rhindan B solution and a concentration of Au nanoparticles solution of 0.5mM;
[0047] (2) Drop 5 μL of Au nanoparticle solution at the inlet of one of the flow channels of the T-shaped flow channel, and at the same time, drop 5 μL of rhinidine B solution at the inlet of the other flow channel;
[0048] (3) Since two wetting gradients are set in the cross flow channel, it can provide a driving force for the movement of the droplet and realize the self-driving of the droplet. The two droplets merge at the intersection of the cross flow channel and move after being fully mixed in the vertical flow channel. Go to the designated detection area at the end of the vertical flow channel, and perform in-situ Raman detection in the detec...
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