Method for improving fermentation yield of natamycin

Abstract

The invention provides a method for improving the fermentation yield of natamycin, and relates to the method for improving the fermentation yield of natamycin. The fermentation yield of the natamycin is improved; meanwhile, the renninum fermentation waste thalli are reasonably used; waste materials are changed into valuable materials. The method comprises the following steps of filtering rice rhizopus nigricans thalli to remove excessive water; preparing suspension; adding lyticase into the suspension for wall breaking enzymolysis to obtain enzymolysis liquid; fermenting 0 to 48h under the existence of natamycin; adding the enzymolysis liquid into the natamycin culture medium; obtaining natamycin after the fermentation. The method has the advantages that the rice rhizopus nigricans fermentation waste thalli for producing chymosin are added into the natamycin fermentation culture medium, so that the yield for producing the strain natamycin can be obviously improved; meanwhile, the problem that the rice rhizopus nigricans fermentation waste thalli are difficult to treat is solved; double benefits are achieved.

Description

technical field [0001] The invention relates to a method for increasing the fermentation yield of natamycin. Background technique [0002] Natamycin, also known as natamycin and pimaricin, is a polyolefin macrolide antifungal agent, which is obligate to inhibit mold and yeast (Cui Xuhai. Natamycin in The latest research status in the food industry. Meat Research. 2009,12:35-38), mainly by Streptomyces natalensis, Streptomyces natalensis, Streptomyces chrysanthosporium (S.gilvosporeus) and S.lydicus and other actinomycetes (Hu Haiyang, Qiao Chunming, Ge Jingping, Ping Wenxiang. The characteristics and production research status of natamycin. China Modern Medicine Application. 2009, 3(2):200-201). The molecular formula of natamycin is C 33 h 47 NO 13 , the molecular weight is 665.725. Natamycin contains a 26-membered macrolide, and there is a seaweed amino sugar linked by a glycosidic bond outside the ring. [0003] Natamycin is stable in nature, but it is difficult to ...

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Problems solved by technology

[0008] 3. Control the growth rate, affect the formation of bacterial types, and thus affect the rheological properties of the fermentation broth;

[0009] 4. Affect oxygen supply level and increase power consumption

[0013] On the other hand, the economic value of the waste Rhizopus miehei after rennet fermentation is not high, and there is no other reasonable way out except for harmless treatment, which will only increase the environmental protection pressure and cost of the enterprise

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