A kind of actinomycetes and application thereof for preventing and treating peanut diseases
A technology for actinomycetes and peanuts, applied in the field of microorganisms, can solve problems such as residual poison, chemical pesticide residue hazards, environmental pollution, etc., and achieve the effects of easy preservation, good development and application prospects, and simple culture conditions.
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[0016] Further illustrate the present invention below in conjunction with accompanying drawing.
[0017] Exploring the isolation and purification of actinomycete LX14 and its inhibition on peanut fungi
[0018] 1. Experimental principle
[0019] Gaoshi No. 1 is a medium capable of cultivating actinomycetes. When the diluted soil is spread flat on Gaoshi No. 1 medium, a single colony can be isolated, which may be roughly isolated as actinomycetes. of single colonies. Then, after size determination by electrophoresis, the sequence of 16srDNA was determined. PDA medium is a medium capable of cultivating both bacteria and fungi. We used PDA medium to culture peanut fungi and actinomycetes at the same time to explore the inhibition of actinomycetes on peanut crown rot, peanut scab, peanut root rot and peanut white silkworm.
[0020] 2. Materials and methods
[0021] 2.1 Test material
[0022] 2.1.1 Soil source and dilution treatment
[0023] Soil was obtained from the experi...
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