Extraction and separation method of total sterol in pumpkin vines

A separation method, pumpkin vine technology, applied in the field of natural medicinal chemistry, can solve the problems such as rare reports on pumpkin vine sterol extraction process, and achieve the effect of high total sterol content, low cost and simple process

Inactive Publication Date: 2016-08-03
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the extraction process of pumpkin vine sterol

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: extract and separate total sterols of pumpkin vine according to the following steps

[0012] (1) Take fresh pumpkin vines and dry them at 70°C, crush them through a 60-mesh sieve to obtain pumpkin vine powder;

[0013] (2) Put the pumpkin vine powder in a heating reflux device, heat and reflux with 90% ethanol at 70°C for 2 hours, filter after heating and reflux, and repeat the reflux of the filter residue 4 times, combine the filtrate, recover the ethanol under reduced pressure and dry it in vacuum to obtain Ethanol extract; wherein the mass volume ratio of pumpkin vine powder and 90% ethanol is 1:20 (g / mL);

[0014] (3) Weigh the ethanol extract, add distilled water according to the mass of ethanol extract and distilled water volume 1:1 (g / mL); then add petroleum ether according to the mass of ethanol extract and petroleum ether volume 1:1 (g / mL) Extraction, after vigorously mixing and standing at room temperature for 24 hours to remove the petroleum et...

Embodiment 2

[0016] Embodiment 2: extract and separate total sterols of pumpkin vine according to the following steps

[0017] (1) Take fresh pumpkin vines and dry them at 70°C, crush them through a 60-mesh sieve to obtain pumpkin vine powder;

[0018] (2) Put the pumpkin vine powder in a heating reflux device, heat and reflux with 90% ethanol at 70°C for 2.5 hours, filter after heating and reflux, and repeat the reflux of the filter residue 4 times, combine the filtrate, recover the ethanol under reduced pressure, and then vacuum dry Obtain ethanol extract; wherein the mass volume ratio of pumpkin vine powder and 90% ethanol is 1:20 (g / mL);

[0019] (3) Weigh the ethanol extract, add distilled water according to the mass of ethanol extract and distilled water volume 1:1 (g / mL); then add petroleum ether according to the mass of ethanol extract and petroleum ether volume 1:1 (g / mL) Extraction, vigorously mixing, standing at room temperature for 24 hours to remove the petroleum ether layer,...

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PUM

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Abstract

The invention provides an extraction and separation method of total sterol in pumpkin vines. The extraction and separation method comprises the following operation steps: (1) taking fresh pumpkin vines to be dried at 70 DEG C, crushing and filtering with a 60-mesh sieve; (2) using ethanol with the mass concentration of 90% for heating reflux at 70 DEG C, and drying an extract to obtain an ethanol extract; (3) adding water to disperse the ethanol extract, extracting with petroleum ether for several times, combining petroleum ether layers, and removing a solvent to obtain a petroleum ether extract; (4) applying the petroleum ether extract to a silica gel column for separation, eluting with methylene chloride and methanol in a volume ratio of 100:1 to remove impurities, using methylene chloride and methanol in the volume ratio of 10:1 as an eluent for elution, collecting eluate, decompressing, concentrating and drying the eluate to obtain the extract of total sterol in the pumpkin vines. The extraction and separation method provided by the invention is simple in process, high in extraction rate, excellent in reproducibility, stable and feasible.

Description

technical field [0001] The invention relates to an extraction and separation method of pumpkin vine total sterols, belonging to the field of natural medicinal chemistry. Background technique [0002] Pumpkin [Cucurbitamoschata (Duch). Poiret] is a plant of Cucurbitaceae Cucurbita. Pumpkin vine, also known as pumpkin vine, Panchang grass, pumpkin seedlings, is an annual vine herb with stems up to 2-5m. It is native to Mexico and Central America. It is widely cultivated all over the world. It was introduced into my country in the Ming Dynasty and is now widely planted in the north and south. . Pumpkin vines are rich in carotene, crude cellulose and other nutrients. They have the effects of clearing the lungs, harmonizing the stomach, dredging the collaterals, and treating tuberculosis with low fever, stomach pain, irregular menstruation, and burns. Phytosterols have many different biological functions, such as being a component of cell membranes and forming adrenocortical hor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/42
CPCA61K36/42A61K2236/333A61K2236/39A61K2236/51A61K2236/55
Inventor 李欢桑建赵利琴鄢贵龙
Owner HUAIYIN TEACHERS COLLEGE
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