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Application of composition in preparing drugs for treating tumors and using method of composition

A technology of tumor drugs and compositions, applied in the field of biomedicine, can solve problems affecting the quality of life of tumor patients, insensitivity, clinical chemotherapy and radiotherapy-sensitive tumor patient death, etc.

Inactive Publication Date: 2016-07-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment options for clinical cancer patients are mainly surgical resection, chemotherapy and radiotherapy. Although with the remarkable achievements in clinical oncology research, the survival period of some cancer patients has been significantly prolonged and the quality of life has been continuously improved. The sensitivity of radiotherapy and its side effects are still an important cause of death in cancer patients
[0003] As one of the clinical first-line chemotherapy drugs for many tumors, including head and neck cancer, lung cancer, liver cancer and lymphoma, cisplatin has a significant anti-tumor effect, but it has obvious side effects including gastrointestinal reactions, kidney toxicity, neurotoxicity and Bone marrow suppression, etc., significantly affect the quality of life of cancer patients
In addition, some tumor patients are prone to resistance and insensitivity to cisplatin therapy, which also prompts the update of clinical chemotherapy drug use methods or the development of new drugs

Method used

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  • Application of composition in preparing drugs for treating tumors and using method of composition
  • Application of composition in preparing drugs for treating tumors and using method of composition
  • Application of composition in preparing drugs for treating tumors and using method of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the antitumor effect of pharmaceutical composition in vitro

[0018]First, four head and neck cancer cell lines SCC4, SCC1, CAL27 and OSCC3 were treated with cisplatin at different concentration gradients (0.5 μM, 1 μM, 2 μM, 4 μM, 8 μM, 16 μM and 32 μM), and the differences were detected by CCK-8 experiment after 48 h. The killing effect of concentration cisplatin draws the activity inhibition curve of tumor cells, and obtains the half (50%) inhibitory concentration (i.e. IC50) and IC25 and IC75 of various cells by Prism5 software analysis, see Table 1:

[0019] Table 1 IC25, IC50 and IC75 of four head and neck cancer cell lines against cisplatin

[0020] μM

[0021] The cisplatin concentration corresponding to IC50, IC25 and IC75 of each cell line was used to treat the corresponding 4 kinds of cells, and three experimental groups were set up respectively: the first group was cisplatin alone treatment group: different concentrations of cisplatin...

Embodiment 2

[0022] Embodiment 2: Anti-solid tumor (head and neck cancer) effect of the tumor chemotherapeutic drug composition in vivo:

[0023] Take 1*10 6 A head and neck cancer tumor cell line SCC4 with good growth in the logarithmic growth phase was implanted in the armpit of BALB / cNude nude mice. After the tumor model was successfully established ( figure 2 A), according to the routine clinical dosage of cisplatin is 75mg / m 2 , while the dose of low concentration cisplatin used is 10-20mg / m 2 , the average value is 15mg / m 2 , in order to convert the concentration used in mice corresponding to this clinical concentration, the following formula should be used:

[0024] Mouse (mg / kg)*(mouse conversion factor / human conversion factor)=human (mg / m 2 ) / person conversion factor

[0025] Among them, the mg / kg-mg / m of mice 2 The conversion factor is 3, human mg / kg-mg / m 2 The conversion factor is 36. The dose of cisplatin used to convert the concentration of cisplatin used in mice corr...

Embodiment 3

[0026] Embodiment 3: Anti-solid tumor (head and neck cancer) effect of the tumor chemotherapeutic drug composition in vivo:

[0027] Take 1*10 6 A head and neck cancer tumor cell line SCC4 with good growth in the logarithmic growth phase was implanted in the armpit of BALB / cNude nude mice. After the tumor model was successfully established ( image 3 A), according to the findings of Example 2: pretreatment of nude mice with 25 μg polyIC, and then combined administration the next day can significantly improve the killing effect of low-concentration cisplatin and reduce the toxic and side effects of this method of treatment. We further combined cetuximab, a commonly used targeted therapy drug for advanced head and neck cancer, to treat tumor-bearing nude mice, and set up three experimental groups ( image 3 B): the first group is the tumor-bearing nude mouse group (Poly(I:C) of the third treatment group in embodiment 2 25 / DDP 2.5 group), the second group was to pretreat tumo...

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Abstract

The invention relates to a pharmaceutical composition for tumor treatment, in particular to a composition of tumor treatment drugs including the clinical first-line chemotherapy drug cisplatin, CDDP or DDP, artificially synthesized double-stranded RNA (polyinosinic-polycytidylic acid, poly IC or PIC) and the clinical targeting drug cetuximab, and the using dosage and method of the composition.In vitro and in vivo experiments both show that the composition has important significance for overcoming tumor chemotherapy-resistance and side effects thereof.

Description

Technical field: [0001] The invention relates to the field of biomedicine, in particular to a tumor chemotherapeutic drug composition as well as dosage and sequence. Background technique: [0002] According to the 2015 China Cancer Registration Annual Report, there were about 3.37 million new cancer cases in my country in 2011, an increase of 280,000 cases compared with 2010. In the past 10 years, the incidence and mortality of cancer in my country have shown a clear upward trend. At present, the treatment options for clinical cancer patients are mainly surgical resection, chemotherapy and radiotherapy. Although with the remarkable achievements in clinical oncology research, the survival period of some cancer patients has been significantly prolonged and the quality of life has been continuously improved. The sensitivity of radiotherapy and its side effects are still an important cause of death in cancer patients. [0003] As one of the clinical first-line chemotherapy drug...

Claims

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Application Information

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IPC IPC(8): A61K33/24A61K39/395A61K49/00A61P35/00A61P35/04C12Q1/02A61K31/713
CPCA61K39/395A61K49/00A61K49/0008G01N33/5011A61K31/713A61K33/24
Inventor 侯亚义丁亮泥艳红胡勤刚任靖张东亚历怡
Owner NANJING UNIV
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