Establishment of squalene as a marker for identification of olive oil and camellia oleifera oil

A technology of camellia oleifera seed oil and identification markers, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no methodological verification, no universal and representative significance, no reliable method for oil extraction, etc., Achieve the effect of reducing the loss of samples, avoiding artificial addition, and reducing the amount of sampling

Active Publication Date: 2018-03-16
ZHEJIANG UNIV +1
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Long Zhenghai and Wang Daoping "Studies on the Chemical Components of Camellia Camellia Seed Oil and Olive Oil" ("Journal of Chinese Cereals and Oils", 2008, Vol. The samples used are all commercial products, each with only one sample size, and there is no reliable method for extracting oil from the original fruit, which has no universal and representative significance, let alone methodological verification

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishment of squalene as a marker for identification of olive oil and camellia oleifera oil
  • Establishment of squalene as a marker for identification of olive oil and camellia oleifera oil
  • Establishment of squalene as a marker for identification of olive oil and camellia oleifera oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Embodiment one: establish standard curve

[0053] Using n-hexane as a solvent, prepare a squalene standard solution with a concentration of 10mg / L-700mg / L. Take the squalene concentration (mg / L) as the abscissa, and the corresponding peak area as the ordinate to make a standard curve ( figure 1 ), the linear regression equation of squalene: y=0.3662x-1.0791 (R 2 = 0.9999). Lower limit of detection (LOD): Taking the concentration corresponding to 3 times the baseline noise as the standard, this method is 2.38 μg / mL.

[0054] When detecting a specific sample, the concentration of the sample can be calculated by substituting the squalene peak area in the sample into the regression equation, and then the concentration can be substituted into the following calculation formula to obtain the corresponding content (μg / g):

[0055]

[0056] In the formula:

[0057] X: squalene content in the original sample oil, in milligrams per gram (mg / g);

[0058] Cs: squalene conten...

Embodiment 2

[0061] Embodiment two: sample collection and determination of squalene content

[0062] 1. Camellia oleifera seed and olive fruit collection and oil extraction

[0063] There are 30 kinds of Camellia oleifera oil and olive oil (Table 1) detected in this embodiment. Among them, camellia seeds numbered 1-21 and olive fruits numbered 23-26 were provided by the Subtropical Forestry Research Institute of the Chinese Academy of Forestry. The camellia seeds came from Jinhua and Jiangxi, Zhejiang, and the olive fruits came from Qingchuan, Sichuan.

[0064] Table 1 Details of samples to be tested

[0065]

[0066] Crush the fresh olive fruit and take out the core, add a small amount of water, thoroughly crush and stir, then centrifuge, and the supernatant is freshly squeezed olive oil. Take an appropriate amount of camellia oleifera seeds and place them in an oven at 50°C for a certain period of time. Take it out of the oven and cool to room temperature. Accurately weigh 6g of th...

Embodiment 3

[0084] Example 3: GC-MS compares squalene in the sample with the standard

[0085] 1. Sample pretreatment

[0086] Take a small amount (100mg of tea oil, 5mg of olive oil) to be tested in a 30mL test tube, add 5mL of 2mol / L KOH-ethanol (5.6g potassium hydroxide, 50mL absolute ethanol) solution; place in a water bath at 85°C After saponification for 1h, remove and cool; add 5mL saturated NaCl solution, and then add 4mL petroleum ether; after fully shaking, let it stand for stratification, take the supernatant in a test tube, add 4mL pure water to wash until neutral, and take the upper liquid in another test tube and add a small amount of anhydrous sodium sulfate; after shaking, centrifuge at 2000r / min for 5min; transfer the supernatant to a new test tube, and blow with nitrogen until there is no liquid; μm organic filter membrane); nitrogen was blown to dryness again, and 100 μL of n-hexane (chromatographically pure) was reconstituted and injected.

[0087] 2. Instrument cond...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses an establishing method of squalene as an identification marker of olive oil and camellia seed oil. The method includes the steps of firstly, preparing a squalene standard product into different concentrations, detecting peak areas of different concentrations through a gas chromatograph, and calculating the standard curve and the calculation formula of the relation of the peak areas and the concentrations; secondly, conducting high-temperature saponification on camellia seed oil and olive oil extracted from camellia seeds and olive fruit respectively, or a trace sample of commercial oil, extracting squalene with petroleum ether, and detecting the peak areas of squalene in samples through the gas chromatograph to obtain the squalene contents of various samples; finally, conducting differential statistics and analysis on the squalene contents of all camellia seed oil and olive oil to obtain the squalene contents of camellia seed oil and olive oil. The sample preprocessing is simple, data is visual and reliable, and repeatability is high.

Description

technical field [0001] The invention relates to the field of oil detection in food detection, in particular, the invention relates to a gas chromatographic detection method and a discrimination standard using squalene content as a basis for identification of olive oil and camellia oleifera oil. Background technique [0002] Olive oil is an ancient oil species native to the Mediterranean. It has various functions such as reducing the occurrence of cardiovascular and cerebrovascular diseases, improving the function of the human digestive system, anti-cancer, anti-aging, promoting blood circulation and nervous system development, and its nutritional value is in the The world has long been recognized, known as "liquid gold", "vegetable oil queen". Camellia oleifera seed oil is an ancient Chinese native oil extracted from the seeds of Camellia oleifera. Its chemical properties, especially the composition of fatty acids, are very similar to olive oil. Because of its functions of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/025
Inventor 沈立荣翟量辛晓璇祝洪刚
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap