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Application of AKR1C1 protein

A protein and lung cancer technology, applied in the application field of AKR1C1 protein, to achieve accurate diagnosis of lung cancer, improve accuracy, and increase in vitro metastasis and invasion capabilities

Inactive Publication Date: 2016-07-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

AKR1C1 protein has a certain relationship with tumor drug resistance, but there is no research report on AKR1C1 protein in metastasis, especially in lung cancer metastasis

Method used

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  • Application of AKR1C1 protein
  • Application of AKR1C1 protein
  • Application of AKR1C1 protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Female nude mice aged 4-6 weeks were used for experiments. The non-small cell lung cancer cell line NCI-H1299 stably and highly expressing AKR1C1 group and the control group were collected by EDTA digestion, washed twice with the culture medium, and injected into the tail vein of nude mice. Each nude mouse was injected with 2 million cells in a volume of 200 μL. After 60 days, they were sacrificed by cervical dislocation and dissected to observe organ metastasis and take pictures. like figure 1 As shown, in the group with high expression of AKR1C1, the liver metastasis in nude mice was significantly higher than that in the control group.

Embodiment 2

[0026] The subcultured lung cancer cells NCI-H1650 and NCI-H1299 were transfected with the AKR1C1 gene (AKR1C1 group) respectively, and a blank control (pcmv6 group) was set. After 24 hours of transfection, the in vitro invasion ability of transfected cells was detected by Transwell method. like figure 2 As shown, after overexpressing the AKR1C1 gene, the in vitro invasion ability of lung cancer cells NCI-H1650 and NCI-H1299 was significantly increased.

Embodiment 3

[0028] In subcultured lung cancer cells A549 and PC-9, RNA interference was used to inhibit the expression of AKR1C1 (AKR1C1 group), and a blank control (NC group) was set. After 24 hours of interference, the in vitro invasion ability of transfected cells was detected by Transwell method. like image 3 As shown, after the interference inhibited the expression of AKR1C1, the in vitro invasion ability of lung cancer cells A549 and PC-9 was significantly reduced.

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PUM

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Abstract

The invention provides application of AKR1C1 protein. The product comprises a product for diagnosing lung cancer and predicting lung cancer metastasis by RT-PCR, real-time quantitative PCR, immunodetection, hybridization in situ or gene chip, and is used for preparing a product for diagnosing lung cancer and preparing a medicine for treating lung cancer. Overexpressed AKR1C1 protein can be used for remarkably improving in-vivo metastasis of lung cancer cells and remarkably enhancing in-vitro metastasis and invasion capacity of lung cancer cells, and AKR1C1 can be used for remarkably inhibiting the in-vitro metastasis and invasion capacity of lung cancer cells by RNA interference suppression. The AKR1C1 protein can be used as specific marker protein for diagnosing lung cancer, predicting lung cancer metastasis and prognosing, so that lung cancer diagnosis can be accurately and quickly performed, the accuracy for diagnosing lung cancer and predicting lung cancer metastasis can be improved, and a new treatment target and new effective medicine are provided to lung cancer prevention and treatment.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and relates to the application of a protein, in particular to the application of an AKR1C1 protein. Background technique [0002] The incidence of lung cancer is increasing year by year, and it has become the malignant tumor with the highest morbidity and mortality in my country since 2012. According to statistics from the National Cancer Center of the United States, 79% of lung cancer patients were in stage II, III, and IV metastases when they saw a doctor; the death toll of lung cancer patients accounted for 26.8% of all cancer patient deaths. The rate was only 17.4%. This shows that the metastasis of lung cancer patients is closely related to the prognosis and survival. These data show that clinical research on lung cancer, especially research on metastasis, has great practical significance and clinical needs. [0003] Human AKR1C1 protein is encoded by the AKR1C1 gene (Genebank No. N...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574G01N33/573A61K45/00A61P35/00A61K31/713
CPCA61K31/713A61K45/00C12Q1/6886C12Q2600/118G01N33/573G01N33/57423G01N2333/904
Inventor 杨波何俏军朱虹应美丹常琳琳
Owner ZHEJIANG UNIV
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