Preparation method of hericium erinaceus protein peptide-selenium chelate

A technology of Hericium erinaceus and chelate, which is applied in the field of preparation of Hericium erinaceus protein peptide-selenium chelate, can solve the problems of low biological effectiveness, small lethal dose, toxic and side effects, etc., and achieve low price and easy to be consumed The effect of high absorption and extraction rate

Inactive Publication Date: 2016-07-20
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Step 1: Superfine Hericium erinaceus, mix the crushed Hericium erinaceus with distilled water at a w / v ratio of 1:50, use 2.0wt% NaOH to adjust the pH to 10.0, ultrasonic power 150W, ultrasonic extraction for 20 minutes , extracted in a water bath for 60 minutes, at a temperature of 60°C, filtered with gauze after extraction, the filtrate was the alkaline-extracted Hericium erinaceus protein solution, adjusted the pH of the alkaline-extracted Hericium erinaceus protein solution to 3.0 with hydrochloric acid for protein precipitation, and centrifuged at 4000r / min for 10min to obtain Precipitated protein, washed with water until neutral, centrifuged again, and freeze-dried the precipitate to obtain Hericium erinaceus protein, with an extraction rate of 80.55%;

[0020] Step 2: Dissolve Hericium erinaceus protein in water to make the protein concentration reach 5.0wt%, adjust the pH of Hericium erinaceus protein solution to 7.0 with NaOH solution, keep the temperature at 50...

Embodiment 2

[0028] Step 1: Superfine Hericium erinaceus, mix the crushed Hericium erinaceus with distilled water at a w / v ratio of 1:50, use 3.0wt% NaOH to adjust the pH to 11.0, ultrasonic extraction for 20 minutes, and water bath extraction for 90 minutes , temperature 100°C, filter with gauze after extraction, the filtrate is the alkaline-extracted Hericium erinaceus protein solution, adjust the pH of the alkaline-extracted Hericium erinaceus protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge to obtain the precipitated protein, wash with water until neutral again Centrifuge, freeze-dry the precipitate to obtain Hericium erinaceus protein, and its extraction rate reaches 85.75%;

[0029] Step 2: Dissolve Hericium erinaceus protein in water so that the protein concentration reaches 1.0wt%, adjust the pH to 8.0, keep the temperature at 40°C, add 1.0wt% neutral protease, hydrolyze for 50min, and inactivate the enzyme to obtain Hericium erinaceus Protein-po...

Embodiment 3

[0038] The first step: superfinely crush Hericium erinaceus, mix the crushed Hericium erinaceus with distilled water at a ratio of 1:60 w / v, gradually add solid ammonium sulfate to make the saturation of ammonium sulfate reach 60wt%, 5000r / min, centrifuged for 5min to obtain Hericium erinaceus protein precipitation, and freeze-dried to obtain Hericium erinaceus protein after desalting by dialysis, and its extraction rate reached 81.15%;

[0039] Step 2: Dissolve Hericium erinaceus protein in water so that the protein concentration reaches 5.0wt%, adjust the pH to 8.0, keep the temperature at 40°C, add 5.0wt% protease, hydrolyze for 50min, and inactivate the enzyme to obtain Hericium erinaceus protein Polypeptide complex solution;

[0040] The third step: the obtained Hericium erinaceus protein polypeptide complex solution is freeze-dried to make Hericium erinaceus protein polypeptide complex freeze-dried powder, then the sodium selenite powder and Hericium erinaceus protein p...

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PUM

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Abstract

The invention discloses a preparation method of a hericium erinaceus protein peptide-selenium chelate. The method comprises the following steps of: extracting protein from hericium erinaceus by use of an alkali-solution and acid-isolation method or an ammonium sulfate sedimentation method; performing restriction digestion on the hericium erinaceus protein by use of alkaline protease, neutral protease and compound protease, and then performing enzyme deactivation so as to prepare hericium erinaceus protein hydrolysate; and chelating selenium in inorganic substance sodium selenite and hericium erinaceus protein peptide so as to obtain the hericium erinaceus protein peptide-selenium chelate. The method is capable of obviously improving the hericium erinaceus protein extraction rate which is up to 80.55 percent-85.75 percent. The prepared mineral element-peptide chelate has a unique chelation system and a transfer mechanism, is easily absorbed, safe and nontoxic, low in price, and capable of simultaneously supplementing amino acid and mineral ions; the selenium content of the prepared hericium erinaceus protein peptide-selenium chelate is 2561-3818mu g / g and provides a theoretical basis and a technical support for developing organic selenium nutrition supplements and functional foods.

Description

technical field [0001] The invention relates to a preparation method of Hericium erinaceus protein peptide-selenium chelate, which belongs to the field of food biotechnology. Background technique [0002] Selenium (Se) is an important mineral element necessary for the human body and has important physiological functions. A large number of existing studies have shown that many diseases are closely related to the nutritional status of Se. The human body mainly obtains selenium through diet, and the method of artificial selenium supplementation is oral selenium-containing preparations. The general method of supplementing selenium is to make oral preparations with inorganic selenium compounds (sodium selenite and sodium selenate). However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited. Studies have shown that organic selenium compound...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/30
CPCC12P21/06C07K1/30
Inventor 赵立娜刘斌陈濠陈紫红肖正林占熺吕旭聪李鑫
Owner FUJIAN AGRI & FORESTRY UNIV
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