Potato culture medium suitable for ustilaginoidea virens spore production and application thereof

Abstract

The invention discloses a potato culture medium suitable for ustilaginoidea virens spore production and an application thereof. Potatoes are prepared into a plate culture medium as a spore production culture medium so as to culture and prepare ustilaginoidea virens thin-wall conidiospore. Preparation for the culture medium and an application method for the culture medium comprise the following steps: 1) preparing the culture medium; 2) inverting a culture flat plate; 3) implanting ustilaginoidea virens; 4) carrying out constant-temperature culture; and 5) collecting a new generation of spores. The potato culture medium has the characteristics and the advantages that: the culture medium is simple in composition; the operation is simple, easy and convenient, and shaking culture equipment is not needed; time of obtaining a great number of spores is short; a high spore production state lasts for a long time; and a spore liquid is relatively pure, is less in mixed mycelia, and is generally suitable for being used in a lot of research work without purifying treatment.

Description

technical field [0001] The present invention relates to phytopathological techniques and microbiological techniques. Specifically, the invention relates to a potato culture medium suitable for spore production of rice false smut and an application method thereof. Background technique [0002] In most fungal disease systems, the propagules of pathogenic bacteria not only have the function of multiplying and expanding their own population, but more importantly, they have the function of interacting with and identifying the host, and initiating the function of infecting the host. Therefore, in many important plant disease research In the process, the propagules of pathogenic bacteria need to be used as test materials. Obviously, an efficient and convenient method for culturing and preparing propagule materials will be very conducive to the smooth development of research work. Rice false smut (caused by Ustilaginoideavirens (Cooke) Takahashi infection) has become a major diseas...

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Problems solved by technology

However, there are also some obvious deficiencies in the technology of preparing spores with this medium. After the sporulation peak, a large number of non-sporulation aerial hyphae grow quickly on the plate colony, resulting in a sharp decline in the number of spores, which cannot be produced in a long period of time. Stable provision of spores for use; moreover, there are still more fragments of mycelium in the new spore liquid collected for preparation of culture results

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