A kind of preservation method of chlorella

Abstract

The invention provides a chlorella preservation method. The preservation method comprises the following steps of: at normal temperature, mixing chlorella concentrated solution with sterile water in a volume ratio of (1-3):1, and then adding glycerol, tea extract and potassium sorbate for preservation, wherein the mass final concentration of glycerol is 1-4%, the mass final concentration of tea extract is 1-4%, and the mass final concentration of the potassium sorbate is 0.04-0.08%. The chlorella preservation method has the advantages that a preservation technology is simple, enlarged production can be easily realized, aiming at solving the problems that chlorella can be easily polluted due to misoperation in a preservation process and important indexes such as biomass, chlorophyll and active polysaccharide of chlorella after resurrection decline quickly, a safe and efficient protective agent is screened and compounded, and storage and transportation can be carried out at normal temperature, so that low temperature energy consumption and transportation inconvenience are avoided.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preserving freshwater chlorella. [0003] (2) Background technology [0004] 1. Introduction to Chlorella [0005] Chlorella is a general unicellular green algae belonging to Chlorophyta, Chlorophyta, Chlorophyceae, and Chlorella genus, and is generally a group of unicellular green algae. Chlorella has an extremely wide distribution range and can grow well in temperate, tropical, freshwater or saltwater. Chlorella has existed for a long time. It is one of the earliest life forms on earth, and it is also the earliest microalgae cultivated by human beings. [0006] Chlorella is a natural nutritionally balanced, green, nutritious and healthy food. The United Nations Health Organization and the Food and Agriculture Organization once praised it as "the ideal food in the 21st century" and "the best food in the 21st century". The chlorophyll in chlorella cells is as high as 4% to 6%, which is the highest cont...

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Problems solved by technology

[0029] In summary, the subculture preservation method is simple and easy, and is usually preserved at room temperature or normal low temperature, but the disadvantage is that the preservation time is short, frequent subculture is required, and pollution and variation are prone to occur, so it can only be used for short-term preservation of algae; dry preservation At present, the method can only be used for some cyanobacteria and a few green algae, and most of them have a very low survival rate; cryopreservation requires more complicated operation techniques and expensive storage equipment, and can only save a small amount of algae species; immobilization technology can save algae species The recovery culture has a fast growth rate, but the operation is cumbersome, the process is easily polluted, and it is not suitable for all algae species

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