A kind of laminarin polysaccharide and its extraction and separation method
A technology of kelp polysaccharide and kelp dry powder, which is applied in the directions of pharmaceutical formulations, organic active ingredients, and medical preparations containing active ingredients, etc., can solve problems such as unseen and rare single polysaccharide components, and achieve the effect of uniform molecular weight distribution.
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Embodiment 1
[0023] A method for separating and extracting laminarin, the specific steps are:
[0024] 1) Take 60 g of dry kelp powder, add 1.5 L of absolute ethanol, bathe in water at 50°C for 4 h, filter, collect the filter residue, and let it stand at room temperature and air-dry to obtain dry kelp powder;
[0025] 2) Take 50 g of decolorized kelp dry powder, add 2.75 L of ultrapure water, place in an autoclave at a pressure of 0.1 MPa, and process for 50 min; filter, collect the filtrate and concentrate to 600 mL;
[0026] 3) Add absolute ethanol to adjust the final volume concentration of ethanol to 25%, 50%, and 75%, carry out graded precipitation, collect ethanol precipitates with a volume concentration of 75%, and dry to obtain laminarin crude extract with a yield of 3.5%;
[0027] 4) Dissolve the crude polysaccharide extract in 0.02 mol / L Tris-HCl buffer (pH 7.5), and separate it by DEAE-Sephadex anion exchange chromatography. The eluent is Tris-HCl buffer containing 2 mol / L NaCl,...
Embodiment 2
[0032] A method for separating and extracting laminarin, the specific steps are:
[0033] 1) Take 60 g of dry kelp powder, add 1.5 L of absolute ethanol, bathe in water at 50°C for 4 h, filter, collect the filter residue, and let it stand at room temperature and air-dry to obtain dry kelp powder;
[0034] 2) Take 50 g of decolorized kelp dry powder, add 3.5 L of ultrapure water, place in an autoclave at a pressure of 0.14 MPa, and process for 60 min, filter, collect the filtrate and concentrate to a volume of 1000 mL;
[0035] 3) Add absolute ethanol to adjust the final volume concentration of ethanol to 25%, 50%, and 75%, carry out fractional precipitation, collect 75% ethanol precipitate, and dry to obtain laminarin crude extract, with a yield of 3.6%;
[0036] 4) Dissolve the crude polysaccharide extract in 0.02 mol / L Tris-HCl buffer solution (pH 7.5), separate it by DEAE-Sephadex ion exchange chromatography, the eluent is Tris-HCl buffer solution containing 1 mol / L NaCl, a...
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