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Laminarin and extraction and separation method thereof

A technology of kelp polysaccharide and separation method, which is applied in the directions of pharmaceutical formulations, organic active ingredients, medical preparations containing active ingredients, etc., can solve problems such as unseen and rare single polysaccharide components, and achieve the effect of uniform molecular weight distribution

Inactive Publication Date: 2016-07-20
FUJIAN HEALTH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on tracking the extraction and separation process of polysaccharides by activity. Moreover, most of the active polysaccharides in water-extracted kelp are crude components, and there are few reports on the activity of a single polysaccharide component. Research reports on the single polysaccharide components of Laminaria laminaria with inhibitory activity on proliferation of vascular smooth muscle cells

Method used

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  • Laminarin and extraction and separation method thereof
  • Laminarin and extraction and separation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for separating and extracting laminarin, the specific steps are:

[0024] 1) Take 60g of kelp dry powder, add 1.5L of absolute ethanol, bathe in water at 50°C for 4 hours, filter, collect the filter residue, and let it stand at room temperature and air-dry to obtain decolorized kelp dry powder;

[0025] 2) Take 50g of decolorized kelp dry powder, add 2.75L of ultrapure water, place it in an autoclave, the pressure is 0.1MPa, and the treatment time is 50min; filter, collect the filtrate and concentrate to 600mL;

[0026] 3) Add absolute ethanol to adjust the final volume concentration of ethanol to 25%, 50%, and 75%, carry out graded precipitation, collect ethanol precipitates with a volume concentration of 75%, and dry to obtain laminarin crude extract with a yield of 3.5%;

[0027] 4) Dissolve the crude polysaccharide extract in 0.02mol / L Tris-HCl buffer (pH7.5), and separate it by DEAE-Sephadex anion exchange chromatography. The eluent is Tris-HCl buffer cont...

Embodiment 2

[0032] A method for separating and extracting laminarin, the specific steps are:

[0033] 1) Take 60g of kelp dry powder, add 1.5L of absolute ethanol, bathe in water at 50°C for 4 hours, filter, collect the filter residue, and let it stand at room temperature and air-dry to obtain decolorized kelp dry powder;

[0034] 2) Take 50g of decolorized kelp dry powder, add 3.5L of ultrapure water, put it in an autoclave, the pressure is 0.14MPa, the treatment time is 60min, filter, collect the filtrate and concentrate to a volume of 1000mL;

[0035] 3) Add absolute ethanol to adjust the final volume concentration of ethanol to 25%, 50%, and 75%, carry out fractional precipitation, collect 75% ethanol precipitate, and dry to obtain laminarin crude extract, with a yield of 3.6%;

[0036] 4) Dissolve the crude polysaccharide extract in 0.02mol / L Tris-HCl buffer (pH 7.5), and separate it by DEAE-Sephadex ion exchange chromatography. The eluent is Tris-HCl buffer containing 1mol / L NaCl. T...

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Abstract

The invention provides laminarin and an extraction and separation method thereof. The method comprises the following steps: extracting a laminarin crude extract having a yield of 3.6 percent, which is superior to that of a traditional water extraction method, by adopting a high-temperature high-pressure water extraction method; performing two-step separation by DEAE-anion exchange chromatography and Sephacryl S-300 gel chromatography to obtain a kelp purified polysaccharide PS-3-1 having a molecular weight of 132 KDa. The prepared kelp purified polysaccharide has free radical scavenging activity, has a scavenging rate for hydroxyl radicals and superoxide anion being over 70 percent, and can be developed into a free radical scavenging drug. Furthermore, the purified polysaccharide has a reproduction inhibiting activity on vascular smooth muscle cells, has a reproduction inhibition rate of over 75 percent, and can be used for preparing a vascular smooth muscle cell reproduction inhibitor medicine.

Description

technical field [0001] The invention relates to the field of extracting active ingredients from seaweed, in particular to a laminarin polysaccharide and an extraction and separation method thereof. Background technique [0002] kelp( Laminaria japonica ) is distributed in the eastern coastal area of ​​my country and is an economical seaweed with the same source of medicine and food. The medicinal use of kelp has a long history, and its medicinal effects are recorded in many classic pharmacopoeias handed down from generation to generation. Polysaccharide is one of the main active components of kelp. Modern pharmaceutical research results show that kelp polysaccharide has biopharmacological activities such as lowering blood fat, anticoagulation, anti-atherosclerosis, and anti-oxidation. It is one of the hot spots in the research of active components of kelp. [0003] Hot water extraction is a commonly used method for extracting active polysaccharides from kelp, which has the...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P39/06A61P9/00
CPCA61K31/715C08B37/0003
Inventor 彭臻菲魏碧娜叶丽颖
Owner FUJIAN HEALTH COLLEGE
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