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Method for extracting general flavones in rhodomyrtus tomentosa leaves

A technology of myrtle leaves and total flavonoids is applied in the field of traditional Chinese medicine development, and can solve the problems of irritating reagents, complicated procedures, harm to the human body, etc., and achieve the effects of less solvent consumption, high extraction rate, and improved yield.

Active Publication Date: 2016-07-20
GUANGXI VETERINARY RES INST
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the most common method for extracting total flavonoids in Chinese herbal medicines is organic solvent extraction. This method has complicated procedures and low extraction rate. Organic solvents such as petroleum ether and ethyl acetate are flammable and explosive. Some reagents are irritating or even toxic, which is harmful to the human body. polluted environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The influence of embodiment 1 different wetting agents on total flavonoids extraction content

[0019] Test method: Weigh 100g of myrtle medicinal powder, soak it with different wetting agents according to the ratio of material to liquid (m / V) 1:1, put the soaked sample into the extraction kettle in the supercritical device, Before the extraction, the carbon dioxide is refrigerated and liquefied, the extraction pressure is set to 20MPa, and the temperature is 30°C. Same as the wetting agent) with a flow rate of 5g / min, CO 2 The flow rate was 20 g / min. The co-solvent is added into the extraction kettle by a high-pressure pump. After the extraction pressure and temperature reach the preset value, start timing. After 1.5 hours of extraction, stop the extraction, close the carbon dioxide cylinder, and open the manual exhaust valve to release the pressure. Extract the obtained myrtle flavonoids from the medium. Then the extract is processed and analyzed to calculate the t...

Embodiment 2

[0022] The influence of embodiment 2 different extraction temperatures on total flavonoids extraction content

[0023] Test method: Weigh 100g of myrtle medicinal powder, soak it with 50% acetone according to the ratio of solid-liquid ratio (m / V) 1:1.5, put the soaked sample into the extraction kettle in the supercritical device, and carry out Before extraction, the carbon dioxide is refrigerated and liquefied, the extraction pressure is set to 20MPa, and the temperature T is adjusted to adjust the opening of the inlet valve of the carbon dioxide into the collection kettle, the pressure of the collection kettle is controlled at 5MPa, and the temperature is controlled at 20°C at the same time. same agent) at a flow rate of 5g / min, CO 2 The flow rate was 20 g / min. The co-solvent is added into the extraction kettle by a high-pressure pump. After the extraction pressure and temperature reach the preset value, start timing. After 1.5 hours of extraction, stop the extraction, close...

Embodiment 3

[0026] Example 3 Different CO 2 Effect of flow rate on extraction content of total flavonoids

[0027] Test method: Weigh 100g of myrtle medicinal powder, soak it with 50% acetone according to the ratio of solid-liquid ratio (m / V) 1:1.5, put the soaked sample into the extraction kettle in the supercritical device, and carry out Before the extraction, the carbon dioxide is refrigerated and liquefied, the extraction pressure is set to 20MPa, the temperature is 30°C, the opening of the inlet valve of the carbon dioxide into the collection kettle is adjusted, the pressure of the collection kettle is controlled at 5MPa, and the temperature is controlled at 20°C at the same time. Wetting agent is the same) the flow rate is 5g / min, CO 2 The flow rate is S. The co-solvent is added into the extraction kettle by a high-pressure pump. After the extraction pressure and temperature reach the preset value, start timing. After 1.5 hours of extraction, stop the extraction, close the carbon ...

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Abstract

The invention relates to a method for extracting general flavones in rhodomyrtus tomentosa leaves.The method comprises the steps of treating carbon dioxide in the super-critical state as an extraction agent, adding cosolvent, selecting appropriate process parameters such as the temperature, pressure, time, cosolvent and concentration, conducting extraction on the raw material rhodomyrtus tomentosa leaves which are dried and cut or pulverized into pieces properly, then conducting pressure-reduction separation on the raw material rich in target extraction substances, and receiving the substances obtained through separation.After the extract is diluted, the flavones content is measured according to a colorimetric method issued in China Veterinary Pharmacopoeia, rutinum is taken as a standard reference material, and the content of extracted flavones is calculated.The method is short in extraction time consumption, low in extraction temperature, high in yield, simple in procedure and convenient in operation, separation is achieved simultaneously in the extraction process, safety and reliability are achieved, no environmental pollution is caused, the obtained product is high in yield and purity, and the method is an efficient and energy-saving flavones extraction and separation method.

Description

technical field [0001] The invention adopts supercritical carbon dioxide technology to extract total flavonoids in myrtle leaves, belongs to the technical field of traditional Chinese medicine development, and is applied to the extraction of total flavonoids in myrtle leaves. Background technique [0002] Myrtle Rhodomyrtustomentosa (Ait.) Hassk. is a Myrtaceae plant, also known as Dangni, Shanren, Gangren, etc. It is mainly distributed in southern my country and is born on red-yellow soil hills. Myrtle is mainly used as medicine with rhizomes, leaves, and fruits. The fruits are edible, sweet, astringent, and flat, and have the effects of nourishing blood and stopping bleeding; the roots have the effects of dispelling wind and activating collaterals, astringent and antidiarrheal; The effect of hemostasis is used for acute gastroenteritis, indigestion, dysentery and other diseases. Myrtle contains flavonoids, tannins, volatile oils, polysaccharides, terpenes and other ingred...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61K127/00
CPCA61K36/61A61K2236/333A61K2236/37
Inventor 孟菲赵武刘伟银慧慧韦晓洁覃振华郭善康姜源明孙建华
Owner GUANGXI VETERINARY RES INST
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