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Papanicolaou staining solution and preparation method thereof

A technology of Papanicolaou staining and staining solution, applied in the field of Papanicolaou staining solution and its preparation, can solve the problems of unstable staining effect, affecting disease diagnosis, complex preparation of Papanicolaou, and achieve bright cell coloring, easy observation and judgment, cell Clearly Structured Effects

Inactive Publication Date: 2016-07-13
天津智达康华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The problem to be solved by the present invention is that the traditional Papanicolaou preparation is complicated, the dyeing is uneven, and the dyeing effect is unstable, which affects the diagnosis of diseases.

Method used

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  • Papanicolaou staining solution and preparation method thereof
  • Papanicolaou staining solution and preparation method thereof
  • Papanicolaou staining solution and preparation method thereof

Examples

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Effect test

Embodiment 1

[0051] A Papanicolaou staining solution, including modified hematoxylin staining solution, EA50 staining solution and Orange G staining solution.

[0052] Wherein, every 100ml of the improved hematoxylin staining solution includes the following components: 0.003g of sodium iodate, 0.1g of hematoxylin, 1g of potassium aluminum sulfate, 10ml of absolute ethanol, 1ml of glacial acetic acid, and the balance is purified water.

[0053] The preparation method of the above-mentioned improved hematoxylin staining solution comprises the following steps:

[0054] (1) Dissolving hematoxylin in absolute ethanol to obtain liquid A;

[0055] (2) dissolving potassium aluminum sulfate in purified water to obtain liquid B;

[0056] (3) Mix liquid A and liquid B to obtain a mixed liquid;

[0057] (4) Add sodium iodate and glacial acetic acid into the mixture, stir to dissolve, add purified water, and mix well to obtain the improved hematoxylin staining solution.

[0058] Each 100ml of EA50 s...

Embodiment approach

[0066] When using the Papanicolaou staining solution provided by the invention, it can be carried out according to the dyeing method of the Papanicolaou staining solution in the prior art or the traditional cylinder staining method. As a kind of implementation mode, the staining can be carried out according to the following steps:

[0067] 1. 95% alcohol fixed;

[0068] 2. Rinse with water;

[0069] 3. Staining with improved hematoxylin staining solution;

[0070] 4. Rinse with water;

[0071] 5. 1% hydrochloric acid, alcohol differentiation;

[0072] 6. Rinse with water;

[0073] 7. Alkaline water (alkaline water is saturated lithium carbonate solution or ammonia solution) turns blue;

[0074] 8. Rinse with water;

[0075] 9. 85% alcohol cleaning;

[0076] 10. 95% alcohol cleaning;

[0077] 11. Staining with orange G staining solution;

[0078] 12. Rinse with 95% alcohol;

[0079] 13. Dyeing with EA50 staining solution;

[0080] 15. Rinse with 95% alcohol;

[0081] 1...

Embodiment 2

[0085] A Papanicolaou staining solution, including modified hematoxylin staining solution, EA50 staining solution and Orange G staining solution.

[0086] Wherein, every 100ml of the improved hematoxylin staining solution includes the following components: 0.005g of sodium iodate, 0.3g of hematoxylin, 3g of potassium aluminum sulfate, 30ml of absolute ethanol, 3ml of glacial acetic acid, and the balance is purified water.

[0087] Each 100ml of EA50 staining solution includes the following components: bright green 0.03g, water-soluble eosin 0.3g, phosphotungstic acid 0.2g, methanol 15ml, glacial acetic acid 2ml, purified water 8ml, and the balance is 95% ethanol.

[0088] Each 100ml of Orange G staining solution includes the following components: 0.2g of Orange G, 0.2g of phosphotungstic acid, 90ml of absolute ethanol, and the balance is purified water.

[0089] In this example, the preparation of the improved hematoxylin staining solution, the EA50 staining solution and the o...

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Abstract

The invention provides a Papanicolaou staining solution and a preparation method thereof, and belongs to the technical field of biology. The Papanicolaou staining solution comprises an improved hematoxylin staining solution, an EA50 staining solution and an orange G staining solution, wherein the improved hematoxylin staining solution comprises the following components: sodium iodate, hematoxylin, aluminum potassium sulfate, ethanol, glacial acetic acid and water; the EA50 staining solution comprises the following components: brilliant green, eosin, phosphotungstic acid, methanol, glacial acetic acid, ethanol and water; the orange G staining solution comprises the following components: orange G, phosphotungstic acid, ethanol and water. The Papanicolaou staining solution performs uniform staining, a cellular structure is clear after staining, cells are stained in bright colors, and observation and decision of clinical pathological sections are easy.

Description

technical field [0001] The invention relates to the technical field of biotechnology, in particular to a Papanicolaou stain solution suitable for clinical use and a preparation method thereof. Background technique [0002] At present, cervicitis, tuberculosis, pleurisy, urinary tract infection and other diseases have become common clinical diseases and frequently-occurring diseases in my country. The incidence of these diseases is high and the cure time is long. How to diagnose these diseases has become a research hotspot. [0003] Liquid-based thin-layer cytology technology can assist clinicians to detect the above-mentioned diseases early and formulate correct treatment plans, so that patients can recover as soon as possible and reduce pain, so it has attracted the attention of researchers and clinicians. [0004] One of the important steps of liquid-based thin-layer cytology technology is to stain cells, and Papanicolaou staining solution is one of the most commonly used ...

Claims

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Application Information

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IPC IPC(8): C12Q1/02
CPCG01N33/5091
Inventor 刘春红
Owner 天津智达康华生物科技有限公司
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