Gene for regulating polar wood yield and application thereof

A gene, poplar technology, applied in the R2R3-MYB gene PagMYB199 and its application fields, can solve the problems of limited transformation effect, and achieve the effect of great research value and application potential

Inactive Publication Date: 2016-07-13
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional improvement measures have very limited effects on the transformation of forest plants with relatively long growth cycles, so it is necessary to provide a genetic material that can promote wood development and increase biomass

Method used

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  • Gene for regulating polar wood yield and application thereof
  • Gene for regulating polar wood yield and application thereof
  • Gene for regulating polar wood yield and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 poplar R2R3-MYB gene PagMYB199 molecular cloning

[0022] 1. Poplar PagMYB199 gene cloning

[0023] Take the poplar stems grown in the culture room for about 6 months, use the CTAB method to extract the total RNA, and use the RT-PCR technology to amplify PagMYB199 The full-length cDNA of the gene was connected into pMD18-T for sequencing (see SEQ ID No.1). The amplification conditions were: pre-denaturation at 95°C for 5min; 35 cycles of 9430s, 40s at 55°C, 1min at 72°C; fragment extension at 72°C for 8min. The amplification primers are:

[0024] PagMYB199cDNAF:5'-ATGGGCAGACAACCTTGTTG-3';

[0025] PagMYB199cDNAR:5'-TTAATGCTTGCCACCCATGTC-3';

[0026] 2. Poplar PagMYB199 Gene sequence information and characteristic analysis

[0027] PagMYB199 The gene contains a coding region of 831bp (see SEQIDNo.1), the molecular weight of the encoded protein is 30.7kDa, and there is a R2R3-MYB conserved domain consisting of 96 amino acids at the N-terminus, which is ...

Embodiment 2

[0030] Example 2 poplar R2R3-MYB gene PagMYB199 transgenic application

[0031] 1. Poplar PagMYB199 Gene overexpression and construction of antisense suppression vector

[0032] poplar PagMYB199 The full-length cDNA of the gene was forwardly and reversely connected to the pCAMBIAL1300 vector, and after the sequence was correct, it contained PagMYB199 Plant overexpression and antisense suppression vectors for genes (see figure 2 ).

[0033] 2. Agrobacterium-mediated transformation of poplar

[0034] Select hybrid poplar leaves that have grown for about 4 weeks and are in good condition, remove the main veins and leaf edges, cut them into leaf disks of the same size, and put them into the differentiation medium for pre-cultivation in the dark for 3 days. At the same time, will PagMYB199 The overexpression and antisense suppression vectors were transformed into Agrobacterium EHA105 and cultured at 28°C until OD 600 =1.0-1.3, prepared as an infection solution. Immerse th...

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Abstract

The invention belongs to the field of genetic engineering, and particularly discloses a gene for regulating polar wood yield and application thereof.A base sequence of an R2R3-MYB gene PagMYB199 is shown as in SEW ID No.1.The application refers to application of the PagMYB199 gene serving as a regulating gene for increasing polar wood yield so as to shorten breeding circle of polar.The PagMYB199 gene is obtained by cloning in hybrid polar (Populus albaXP.glandulosa cv''84k') and has wide application prospect and great economic value.

Description

technical field [0001] The invention belongs to the field of genetic engineering, specifically a R2R3-MYB gene PagMYB199 and its application. Background technique [0002] Poplar has the characteristics of small genome, easy transformation, and fast growth. It is a model plant for the study of forest genetic development and genetic engineering improvement. It is also an important economic tree species. It is widely planted in my country and is mainly used as papermaking, construction materials and energy raw materials. . However, for a long time, poplar has had outstanding problems such as long breeding cycle and low wood yield. Therefore, excavating and utilizing important genes related to the regulation of poplar biomass (wood yield) not only helps to deepen the understanding of wood molecular regulation mechanism, but also provides theoretical basis and application for increasing wood yield and fiber content of poplar guide. [0003] Poplar R2R3-MYB gene family contain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
CPCC07K14/415C12N15/8262
Inventor 周功克柴国华唐贤丰陈艳祁广王殿贺郭
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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