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Method for fast detection of butylated hydroxyanisole (BHA), 2, 6-ditertbutyl-4 methylphenol (BHT) and tert-butylhydroquinone (TBHQ) of plant oil sample and pre-treatment method

A vegetable oil and sample technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of low recovery rate, cumbersome operation, high labor cost, and achieve the effect of reducing contact time, simple operation steps, and reducing processing cost.

Inactive Publication Date: 2016-07-06
COFCO GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pretreatment time of this method is long, the operator has a long contact time with the organic solvent, the hazard is great, and the operation is also cumbersome, and the recovery rate is low
[0005] It can be seen that the pretreatment method and corresponding detection method of BHA, BHT, TBHQ in the vegetable oil sample in the above method have the following defects: (1) the extraction steps are cumbersome, time-consuming, and the recovery rate is low, which cannot cope with emergencies Rapid detection; (2) Due to the cumbersome processing process, the operator has a long contact time with the organic solvent, which is harmful; (3) Due to the long processing process, the labor cost is high

Method used

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  • Method for fast detection of butylated hydroxyanisole (BHA), 2, 6-ditertbutyl-4 methylphenol (BHT) and tert-butylhydroquinone (TBHQ) of plant oil sample and pre-treatment method
  • Method for fast detection of butylated hydroxyanisole (BHA), 2, 6-ditertbutyl-4 methylphenol (BHT) and tert-butylhydroquinone (TBHQ) of plant oil sample and pre-treatment method
  • Method for fast detection of butylated hydroxyanisole (BHA), 2, 6-ditertbutyl-4 methylphenol (BHT) and tert-butylhydroquinone (TBHQ) of plant oil sample and pre-treatment method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 0.2g vegetable oil sample, 6g neutral aluminum oxide, 15mL methanol and BHA, BHT, TBHQ standard solution (three concentrations are 100mg / L) 200μL are mixed in a 50mL centrifuge tube to obtain the mixture; Under the condition of a power of 25W, the obtained mixture was vortexed for 1 min to obtain a supernatant; an accurate amount of 7.5 mL of the obtained supernatant was concentrated to 0.2 mL, and then dried by nitrogen blowing, and then Redissolve with 1 mL of methanol solvent to obtain the test solution.

[0060] The obtained liquid to be tested was subjected to chromatographic analysis with an UltiMate3000 liquid chromatograph. According to the peak area of ​​chromatographic analysis, the concentration of BHA, BHT, TBHQ is obtained by linear equation, and then the recovery rate of BHA, BHT, TBHQ is calculated, and the recovery rate is 80%, 77%, 80% respectively.

Embodiment 2

[0062] 0.2g vegetable oil sample, 12g neutral aluminum oxide, 25mL methanol and BHA, BHT, TBHQ standard solution (three concentrations are 100mg / L) 200 μ L are mixed in the centrifuge tube of 50mL, obtain mixture; Under the condition of a power of 30W, the obtained mixture was vortexed for 3 minutes to obtain a supernatant; an accurate amount of 12.5mL of the obtained supernatant was concentrated to 0.2mL, and then dried by nitrogen blowing, and then Redissolve with 1 mL of methanol solvent to obtain the test solution.

[0063] The obtained liquid to be tested was subjected to chromatographic analysis with an UltiMate3000 liquid chromatograph. According to the peak area of ​​chromatographic analysis, the concentration of BHA, BHT, TBHQ is obtained by linear equation, and then the recovery rate of BHA, BHT, TBHQ is calculated, and the recovery rate is 86%, 82%, 87% respectively.

Embodiment 3

[0065] 0.2g vegetable oil sample, 10g neutral aluminum oxide, 20mL methanol and BHA, BHT, TBHQ standard solution (three concentrations are 100mg / L) 200 μ L are mixed in the centrifuge tube of 50mL, obtain mixture; (2) vortex Under the condition that the power of vortex oscillation is 40W, the resulting mixture was vortexed for 2min to obtain a supernatant; accurately measure 10mL of the obtained supernatant, concentrate it to 0.2mL, and then dry it by blowing dry with nitrogen , and then redissolved with 1mL of methanol solvent to obtain the test solution;

[0066] The liquid to be tested obtained in was subjected to chromatographic analysis with an UltiMate3000 liquid chromatograph. According to the peak area of ​​chromatographic analysis, the concentration of BHA, BHT, TBHQ is obtained by linear equation, and then the recovery rate of BHA, BHT, TBHQ is calculated, and the recovery rate is 94%, 90%, 95% respectively.

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Abstract

The invention relates to the field of food additive detection. The invention relates to a pre-treatment method for fast detection of butylated hydroxyanisole (BHA), 2, 6-ditertbutyl-4 methylphenol (BHT) and tert-butylhydroquinone (TBHQ) in a plant oil sample. The pre-treatment method comprises 1, mixing a plant oil sample, an adsorbent and an extraction solvent to obtain a mixture, 2, carrying out vortex oscillation on the mixture obtained through the step 1 to obtain a supernatant and 3, concentrating the supernatant obtained through the step 2 to obtain a solution to be detected. The invention also relates to a method for fast detection of BHA, BHT and TBHQ in a plant oil sample through the pre-treatment method. The pre-treatment method has a high BHA, BHT and TBHQ yield, fast and simple processes, small process pollution and a low cost.

Description

technical field [0001] The invention relates to the field of detection of antioxidants in vegetable oil samples, in particular to a pretreatment method for rapid detection of antioxidants in vegetable oil samples. Background technique [0002] Tert-butylhydroxyanisole (BHA), 2,6-di-tert-butylhydroxycresol (BHT), and tert-butylhydroquinone (TBHQ) are commonly used synthetic antioxidants in oils, which can prevent or delay Oxidation and deterioration of oil, preventing toxin production, maintaining the stability of nutrients and fat-soluble vitamins in oil. Excessive addition of these fat-soluble phenolic antioxidants will cause harm to human health: for example, large doses of BHA can cause cancer, BHT is suspected of inhibiting the activity of human respiratory enzymes, and TBHQ has the risk of teratogenic and carcinogenic. Therefore, the amount of these antioxidants added to food has attracted increasing attention. In this regard, the national food additive use standard G...

Claims

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Application Information

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IPC IPC(8): G01N30/06
Inventor 王浩杨永坛杨悠悠刘佟
Owner COFCO GROUP
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