Preparation method of oviductus ranae polypeptide
A technology of Rana Rana oil and solution method is applied in the field of preparation of Rana Rana oil polypeptide, which can solve the problems of high price and staying in the laboratory stage.
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Embodiment 1
[0015] (1) Enzymatic hydrolysis: Using forest frog oil as the main raw material, the compound enzymatic hydrolysis method is used for controllable enzymatic hydrolysis. The obtained enzymatic hydrolysis solution is slightly yellow in color, slightly fishy, slightly sour and bitter, and the degree of hydrolysis is about 40. %, the molecular weight of the hydrolyzed peptide is mainly distributed between 1000~7000 Daltons;
[0016] (2) Blending: Weigh 5-20% maltodextrin, 1-10% β-cyclodextrin, 5-20% whey protein powder WPI95, 1-20% concentrated whey protein powder WPC80, respectively, to clean In the drying container, slowly add to a certain quality of enzymolysis solution under the condition of sufficient stirring, and magnetically stir until it is completely dissolved;
[0017] (3) Preparation of emulsifier: Dissolve monoglyceride with a small amount of deionized water at room temperature, adjust it into a thin paste, heat in a water bath to 75℃-80℃, shake vigorously with a shak...
Embodiment 2
[0021] (1) Enzymatic hydrolysis: Using forest frog oil as the main raw material, the compound enzymatic hydrolysis method is used for controllable enzymatic hydrolysis. The obtained enzymatic hydrolysis solution is slightly yellow in color, slightly fishy, slightly sour and bitter, and the degree of hydrolysis is about 40. %, the molecular weight of the hydrolyzed peptide is mainly distributed between 1000~7000 Daltons;
[0022] (2) Blending: accurately weigh 20% maltodextrin, 4% β-cyclodextrin, 15% isolated whey protein powder WPI95, 20% concentrated whey protein powder WPC80 in a clean and dry container. Under conditions, slowly add to a certain quality of enzymatic hydrolysis solution, magnetically stir until completely dissolved;
[0023] (3) Preparation of emulsifier: Dissolve monoglyceride with a small amount of deionized water at room temperature, adjust it into a thin paste, heat in a water bath to 75℃-80℃, shake vigorously with a shaker to dissolve it to form a white ...
Embodiment 3
[0027] (1) Enzymatic hydrolysis: Using forest frog oil as the main raw material, the compound enzymatic hydrolysis method is used for controllable enzymatic hydrolysis. The obtained enzymatic hydrolysis solution is slightly yellow in color, slightly fishy, slightly sour and bitter, and the degree of hydrolysis is about 40. %, the molecular weight of the hydrolyzed peptide is mainly distributed between 1000~7000 Daltons;
[0028] (2) Blending: accurately weigh 25% maltodextrin, 5% β-cyclodextrin, 20% isolated whey protein powder WPI95, 20% concentrated whey protein powder WPC80 in a clean and dry container. Under conditions, slowly add to a certain quality of enzymatic hydrolysis solution, magnetically stir until completely dissolved;
[0029] (3) Preparation of emulsifier: Dissolve monoglyceride with a small amount of deionized water at room temperature, adjust it into a thin paste, heat in a water bath to 75℃-80℃, shake vigorously with a shaker to dissolve it to form a white ...
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