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Polypeptide zinc chelate prepared with soy protein isolate as base and preparation method

A soybean protein isolate and chelate technology, applied in animal feed, additional food elements, applications, etc., can solve the problems of amino acid imbalance, low polypeptide content, low polypeptide content, etc., and achieve high social and economic benefits, purity and content. Enhance and improve the effect of immune function

Inactive Publication Date: 2016-07-06
SHANGHAI YUANYAO BIOLOGICAL SHARES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are methods of enzymatically hydrolyzing some protein waste or soybean meal and cottonseed meal to prepare peptides or small peptides to chelate zinc, but there are many problems in these methods, which will eventually lead to product quality problems
For example, a small peptide chelated trace element feed additive and its production process are patented (publication number: 102657285A). The amino acid in the hydrolyzed protein waste liquid used in it is unbalanced, and the heavy metals will exceed the standard. The pH8.0 is used for chelation, and the product contains A considerable part will exist in the form of zinc hydroxide, and the spray drying temperature is too high, which will affect the product quality; patent a preparation method and application of a small peptide chelated zinc complex (publication number: 102827909A), which uses fermented enzymatic hydrolysis of soybean meal, etc. After the protein raw material is chelated with metal salts, there is no special treatment in the later stage. The polypeptide molecular weight distribution in the mixture is wide, the production process is not easy to control, the product stability is poor, and the effective polypeptide content is low. These problems need to be corrected urgently; the patent uses soybean meal as the The small peptide chelate prepared from the base material and its preparation method (publication number: 102669418A), which uses soybean meal as the substrate, the content of the peptide obtained in the later stage is less, and the content of the peptide chelated zinc in the finished product is relatively low
[0005] The production process of polypeptide zinc chelate produced by the prior art is relatively simple, the quality of the finished product is unstable, the content of chelated zinc in the finished product is low, some products are of poor quality, the taste of the product is not good, and the price is expensive. These problems need to be solved urgently

Method used

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  • Polypeptide zinc chelate prepared with soy protein isolate as base and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: preparation is the polypeptide zinc chelate of substrate with soybean protein isolate, comprises the following steps:

[0042] 1) Preparing medium: use soybean protein concentrate as the base material, prepare medium with ultra-fine powder soybean meal and glucose, the weight ratio of soybean protein concentrate: soybean meal: glucose is 85:5:10, soybean meal and glucose provide carbon source and Trace elements required for microbial growth, prepared and mixed evenly, and sterilized by steaming at 105°C;

[0043] 2) Fermentation stage: Prepare three-stage expansion culture strains, in which lactic acid bacteria: yeast = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, according to the ratio of material to water 1:2.0, mix with appropriate water and mix with the prepared medium evenly, control the fermentation temperature at 37-42°C, and the fermentation time is 42h;

[0044] 3) Enzymolysis stage: mix neutral protease and flavor protease w...

Embodiment 2

[0048] Embodiment 2: preparation is the polypeptide zinc chelate of substrate with soybean protein isolate, comprises the following steps:

[0049] 1) Preparation of medium: use soybean protein concentrate as the base material, and prepare medium with ultrafine powder soybean meal and glucose, wherein the weight ratio of soybean protein concentrate: soybean meal: glucose is 85:10:5, and soybean meal and glucose provide carbon Sources and trace elements required for microbial growth, prepared and mixed evenly, and sterilized by steaming, the sterilization temperature is 105°C;

[0050] 2) Fermentation stage: Prepare three-stage expansion culture strains, in which lactic acid bacteria: yeast = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, according to the ratio of material to water 1:1.2-1:2.5, mix with appropriate water and mix with the prepared medium evenly, control the fermentation temperature at 37-42°C, and the fermentation time is 42h;

[0051] 3) Enz...

Embodiment 3

[0055] Embodiment 3: preparation is the polypeptide zinc chelate of substrate with soybean protein isolate, comprises the following steps:

[0056] 1) Preparation of medium: use soybean protein concentrate as the base material, and prepare medium with ultrafine powder soybean meal and glucose, wherein the weight ratio of soybean protein concentrate: soybean meal: glucose is 85:8:7, soybean meal and glucose provide carbon Sources and trace elements required for microbial growth, prepared and mixed evenly, and sterilized by steaming, the sterilization temperature is 105°C;

[0057] 2) Fermentation stage: Prepare three-stage expansion culture strains, in which lactic acid bacteria: yeast = 1:3, and the inoculation concentration is 4×10 7 -9×10 7 cfu / ml, according to the ratio of material to water 1:1.2-1:2.5, mix with appropriate water and mix with the prepared medium evenly, control the fermentation temperature at 37-42°C, and the fermentation time is 42h;

[0058] 3) Enzymoly...

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Abstract

The invention discloses polypeptide zinc chelate prepared with soy protein isolate as base and a preparation method. The polypeptide zinc chelate is prepared by soy protein isolate as base through fermenting, enzymolysis, nanofiltering, chelating and drying. After fermenting, enzymolysis and filtering of the soy protein isolate, polypeptide is generated as chelate to obtain polypeptide zinc chelate.Purity and content of the polypeptide zinc chelate are higher than those of the like polypeptide chelate zinc, and the polypeptide zinc chelate can be directly added into fodder completely instead of pure microelement additive and is excellent in application effect. The polypeptide zinc chelate is good in food calling effect, stable and contains much multifunctional polypeptide.

Description

technical field [0001] The invention relates to the field of feed production, in particular to a polypeptide zinc chelate prepared with soybean protein isolate as a substrate and a preparation method thereof. Background technique [0002] Zinc is an essential trace element in animals. It is related to the activity of nearly 300 enzymes in the body. Some enzymes use zinc as their structural component, and some use zinc as their cofactor. Zinc regulates and controls the structure and function of these enzymes. function, thereby affecting many metabolic functions of the body; at the same time, zinc can also change the structure and function of the epithelial cells of the digestive tract, regulate the activity of various zinc-containing digestive enzymes, and affect the digestion of pigs (Calm, 2003). The zinc supplements traditionally used in pig feed are mainly inorganic zinc (such as zinc sulfate, zinc oxide). Oxalic acid, phosphate ions) combine to form insoluble salts or p...

Claims

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Application Information

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IPC IPC(8): A23K20/20A23K20/142
Inventor 郭子好方华季春源朱校适孙中超
Owner SHANGHAI YUANYAO BIOLOGICAL SHARES
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