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Polypeptide fishy-smell-removing method

A deodorizing and alcohol-soluble technology, applied in the field of deep processing of scallops, can solve the problems of heavy fishy smell, restricted application, poor product flavor, etc., and achieves the effects of increasing purity and content, enhancing hydrophobicity, and improving deodorizing effect.

Inactive Publication Date: 2018-10-12
金华市铁骑士生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, like other protein hydrolysates, the scallop by-product hydrolyzate has a strong fishy smell, and the product flavor is not good, which restricts its application in the food industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A method for deodorizing polypeptides, comprising the following steps:

[0019] 1) Dialyze the scallop enzymatic solution with a 10ku pore diameter fiber dialysis bag. The solid-liquid ratio in the prepared enzymatic solution is 1:150, the temperature is 1°C, and the dialysis is performed for 72 hours. The dialysate is changed every 24 hours and stored at low temperature; 2) After dialysis, the enzymatic hydrolysis solution passes through the ultrafiltration membrane and becomes a polysulfone hollow fiber, with an effective membrane area of ​​0.3m 2 , the inlet pressure is 0.13MPa, the outlet pressure is 0.05MPa, the working temperature is 35°C, the pH is 7, the permeate is collected, and the permeate is returned to the circulating liquid storage tank for ultrafiltration again until there is no unpermeated component to obtain the peptide liquid ;3) Add 0.1‰ of ethyl maltol and 0.01‰ of β-cyclodextrin to the peptide solution after ultrafiltration, and then let it stand a...

Embodiment 2

[0021] A method for removing fishy smell of polypeptides, comprising: enzymatic hydrolysis, dialysis, ultrafiltration to remove fishy smell, masking to remove fishy smell, and alcohol dissolution to remove fishy smell, specifically including the following steps:

[0022] 1) Enzymolysis: After cooking the fresh scallops, remove the shells and take out the scallops, put the remaining by-products into the pot, cook for 45 minutes, and pour them into a homogenizer to fully homogenize them as enzymatic hydrolysis substrates; during the enzymatic hydrolysis process, set In a constant temperature water bath, add 3000U / g neutral protease and 3500U / g papain, enzymatically hydrolyze for 5h under the conditions of a solid-liquid ratio of 1g:8mL, a temperature of 50°C, and a pH of 7, and then adjust the pH to 6. Add 1500U / g flavor protease to enzymatically hydrolyze and continue enzymatic hydrolysis for 3h, inactivate the enzyme in a boiling water bath for 20min, after the enzymolyzed solu...

Embodiment 3

[0028] A method for removing fishy smell of polypeptides, comprising: dialysis, ultrafiltration to remove fishy smell, masking to remove fishy smell, and alcohol solution to remove fishy smell, specifically including the following steps:

[0029] Dialysis: Dialyze the scallop enzymatic solution with a 10ku aperture fiber dialysis bag. The solid-liquid ratio in the prepared enzymatic solution is 1:160, the temperature is 1.5°C, and the dialysis is performed for 84 hours. The dialysis solution is changed every 24 hours. Effectively reduce the ash content in the scallop polypeptide, and its ash content can be reduced to less than 3%, increasing the purity and content of low-molecular-weight proteins and polypeptides in the enzymatic hydrolysis solution;

[0030] Ultrafiltration deodorization: pass the dialyzed enzymatic hydrolysis solution through the ultrafiltration membrane to form a polysulfone hollow fiber, with an effective membrane area of ​​0.4m 2 , the inlet pressure is 0...

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PUM

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Abstract

The invention discloses a polypeptide fishy-smell-removing method. The polypeptide fishy-smell-removing method comprises the steps of dialysis, ultra-filtration fishy smell removing, shielding fishy smell removing and alcohol-soluble fishy smell removing. Firstly, a chlamys farreri enzymatic hydrolysate is dialysed to remove ash content; then the enzymatic hydrolysate is subjected to ultra-filtration fishy smell removing through a polysulfone hollow fiber ultra-filtration membrane; then ethyl maltol, beta-cyclodextrin and pantolactone are added into a peptide solution subjected to ultra-filtration for shielding fishy smell removing; and finally, an ethanol solution is added for centrifuging to take sediment, and then polypeptides from chlamys farreri are obtained. The polypeptide fishy-smell-removing method has the beneficial effects that the polypeptide fishy-smell-removing method is easy to operate, fishy smell substances of amine, aldehyde, ketone and the like in a polypeptide solution are removed thoroughly, adsorption and wrapping of the beta-cyclodextrin for the aldehyde, ketone and amine type substances can be greatly improved through the pantolactone, 3-hydroxy-5-methyl-2,4-imidazolone in alcohol-soluble fishy smell removing facilitates acceleration separating of the aldehyde and amine type substances, and meanwhile degradation of the polypeptides and proteins can be lowered.

Description

technical field [0001] The invention relates to the field of deep processing of scallops, in particular to a method for deodorizing polypeptides. technical background [0002] Scallop is the synonym for the bivalve mollusk of the genus Scallop, and there are about 400 species. More than 60 species of this family are one of the important marine fishery resources all over the world. The shell, meat and nacre have extremely high utilization value. Many scallops are eaten as a delicacy. Scallops are generally not exposed when the sea water is low tide, so it is more troublesome to catch them. On the coast of my country, scallop fishing is mainly in the north, and only Dongchu Island, a little north of Shidao in Shandong Province, and Changshan Islands in Changhai County, Liaoning Province are the most famous. Scallop shells have various colors and neat and beautiful ribs, so they are good materials for making shell carving crafts. People who go to the beach to work, travel ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A23L33/18A23L5/20
CPCC12P21/06A23L5/20A23L5/27A23L5/273A23L33/18A23V2002/00A23V2250/55
Inventor 全盈园
Owner 金华市铁骑士生物科技有限公司
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