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Preparation method of fish scale keratin

A technology of keratin and fish scales, which is applied in the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problem of low added value, lack of means of resource utilization, and insufficient utilization of nutritional functional substances of fish scales, etc. problem, achieve high hydrolysis rate, high content and purity, and good quality

Inactive Publication Date: 2017-06-13
WEIHAI YUWANG GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country lacks effective means of utilizing these resources, and the nutritional and functional substances in fish scales have not been fully utilized, and the added value is relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] After washing 1 part by weight of fish scales, it is added to 8 parts by weight of a hydrochloric acid solution with a mass concentration of 3%, stirred at 250 r / min for 2 hours at room temperature, and rinsed with deionized water to neutrality to obtain delimed scales; Add 1 part of delimed fish scales to 6 parts by weight of deionized water, heat to 100°C and keep for 1h; continue to add 1 part by weight of delimed fish scales, continue to keep at 100°C and keep for 2h to obtain a collagen solution with solid residue Centrifugal separation to obtain a solid residue, after adding 3 parts by weight of water to 1 part by weight of the solid residue, adding a composite protease with a total weight of 3‰ of the solid residue and water, and hydrolyzing at 55° C. for 2 hours to obtain crude fish scale keratin; The crude fish scale keratin product was added with 0.5% activated carbon of the crude fish scale keratin quality, adsorbed at 60°C for 40 minutes, decolorized and deodo...

Embodiment 2

[0042] After washing 2 parts by weight of fish scales, add it to 10 parts by weight of 5% hydrochloric acid solution, stirring at 300r / min for 1 hour at room temperature, and washing with deionized water to neutrality to obtain deliming fish scales; Add 1 part of delimed fish scales to 8 parts by weight of deionized water, heat to 100°C and keep for 1h; continue to add 1 part by weight of delimed fish scales, keep at 100°C and keep for 1h to obtain a collagen solution with solid residue ; Continue to add 1 part by weight of delimed fish scales, continue to keep at 100°C and keep for 2 hours to obtain a collagen solution with solid residue; centrifuge to obtain a solid residue, add 3 parts by weight of water to 1 part by weight of solid residue, Add a composite protease with a total weight of 1.5‰ of solid residue and water, hydrolyze it at 50℃ for 4h to obtain crude fish scale keratin; add 1% active carbon of crude fish scale keratin to the crude fish scale keratin, and absorb a...

Embodiment 3

[0045] After washing 2 parts by weight of fish scales, add 9 parts by weight of a hydrochloric acid solution with a mass concentration of 4%, stirring at 250 r / min for 2 hours at room temperature, and washing with deionized water to neutrality to obtain deliming fish scales; Add 1 part of delimed fish scales to 8 parts by weight of deionized water, heat to 100°C and keep for 2h; continue to add 1 part by weight of delimed fish scales, continue to keep at 100°C and keep for 1h to obtain a collagen solution with solid residue ; Continue to add 1 part by weight of deliming fish scales, continue to keep at 100°C and keep for 2 hours to obtain a collagen solution with solid residue; centrifuge to obtain solid residue, add 5 parts by weight of water to 1 part by weight of solid residue, Add a compound protease with a total weight of 2.5‰ of solid residue and water and hydrolyze it at 55℃ for 3h to obtain crude fish scale keratin; add 0.8% active carbon of crude fish scale keratin to t...

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Abstract

The invention provides a preparation method of fish scale keratin. The preparation method comprises the following steps: cleaning and deashing fish scales, and flushing to be neutral to obtain deashed fish scales; adding 6 to 8 weight parts of deionized water into 1 to 2 weight parts of the deashed fish scales, heating to be 90 to 100DEG C and insulating for 1 to 2 hours; continuously adding 1 to 2 weight parts of the deashed fish scales, and continuously insulating at the temperature of 90 to 100DEG C for 1 to 4 hours; separating to obtain a collagen solution and solid residues fish scale keratin; adding compound protease into the collagen solution, and hydrolyzing for 2 to 4 hours to obtain a fish scale keratin crude product; purifying the fish scale keratin crude product and then carrying out spray drying to obtain the fish scale keratin. According to the preparation method disclosed by the invention, the deashed fish scales are added in a process of heating to separate collagen and keratin by multiple times, so that the purity and the yield of the keratin are high, a Maillard reaction is inhibited, the generation of side products is less, the yield of the fish scale keratin of which the molecular weight is smaller than 1000Da is greater than 85 percent, and the purity of the fish scale keratin reaches 99 percent or above.

Description

Technical field [0001] The invention belongs to the field of keratin preparation methods, and in particular relates to a method for preparing fish scale keratin through a combination of multi-stage countercurrent extraction low-temperature Maillard reaction, synthase enzymatic hydrolysis and ion exchange purification processes. Background technique [0002] During the processing of fish products, scraps such as scales and viscera are produced, which account for 10%-15% of the total weight of the fish, of which the scales account for about 3%-5%. Our country lacks effective means of using these resources, the nutritional functional substances in fish scales are not fully utilized, and the added value is relatively low. [0003] Fish scales are rich in crude protein and ash, and the crude protein content accounts for 39.90-78.60% of the dry matter. The protein in fish scales is mainly collagen and hard protein. The content of ichthyin is 4.454-8.29% of the dry matter, and ichthyrin...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/14
Inventor 苗强
Owner WEIHAI YUWANG GROUP
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