Mulberry parasitical phellinus strain wild environment returning rejuvenation culture method
A cultivation method, Phellinus technology, applied in the field of microbial cultivation, can solve the problems such as strain decline, and achieve the effect of solving degradation, stable and reliable strain source, and stable product quality
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[0012] A kind of mulberry tree parasitic Phellinus bacterium classification returns wild environment rejuvenation culture method, comprises the following steps successively:
[0013] A. Collection of wild Phellinus fruiting bodies: select fresh, complete, not yet lignified, and undamaged fruiting bodies, store at 4°C, and separate within 24 hours;
[0014] B. Isolation and purification of Phellinus strains: Rinse the surface of the fruiting body with sterile water to remove impurities in a sterile operating table, disinfect the surface of the fruiting body with 75% alcohol, cut out Phellinus 2 × 5mm tissue pieces with a scalpel and place them on a solid In plate medium, cultivate in a constant temperature incubator at 20-30°C. After Phellinus mycelium begins to grow, pick the vigorously growing mycelium at the top and further purify and cultivate it to obtain a purified Phellinus strain;
[0015] The Phellinus linteus (Inonotus linteus) used in the present invention comes fro...
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