Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Building method of mouse liver apoptosis animal model induced by nanometer zinc oxide

A technology of nano-zinc oxide and animal model, applied in animal husbandry and other directions, can solve the problems of lack of systematic research on the influence, absorption, distribution, metabolism and excretion of nano-zinc oxide, and achieve the effect of simple preparation

Inactive Publication Date: 2016-06-29
ZHEJIANG PROVINCIAL HOSPITAL OF TRADITIONAL CHINESE MEDICINE
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in stark contrast to the rapid marketization of nano-zinc oxide, people still know little about the possible negative biological effects or toxic side effects of nano-zinc oxide entering the body through different routes, and the absorption and distribution of nano-zinc oxide in the body There is also a lack of systematic research on in vivo behaviors such as metabolism, excretion, etc., which will have a potential impact on the further application of nano-zinc oxide

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Building method of mouse liver apoptosis animal model induced by nanometer zinc oxide
  • Building method of mouse liver apoptosis animal model induced by nanometer zinc oxide
  • Building method of mouse liver apoptosis animal model induced by nanometer zinc oxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The poisoning experiment of embodiment 1 mouse

[0025] (1) Healthy 2-3 month-old normal-grade ICR mice were randomly divided into 5 model groups (model group 1-model group 5) and 1 normal saline control group, with 14 mice in each group, half male and half male, Raise in separate cages.

[0026] (2) Five model groups of mice were intraperitoneally injected with 50mg / kg, 75mg / kg, 100mg / kg, 125mg / kg and 150mg / kg of nano-zinc oxide (nano-zinc oxide was 10mg / mL nano-zinc oxide suspension) for 30 consecutive days to induce mouse liver cell apoptosis; mice in the control group were intraperitoneally injected with normal saline at a dose of 25 mL / kg for 30 consecutive days.

Embodiment 2

[0027] Example 2 Effects of different doses of nano-zinc oxide on mouse serum zinc and liver tissue SOD activity, MDA, CAT, NO content

[0028] Using the method of Example 1, the mice were intraperitoneally injected with different doses of nano-zinc oxide suspension, and on the third day after the injection, the serum zinc content of the mice and the SOD activity, MDA, CAT, and NO contents of the liver tissue were detected.

[0029] (1) Examination of liver cell biomarkers: detection of SOD activity, MDA, CAT and NO contents in liver tissue.

[0030] The activity of SOD was measured by xanthine oxidase method, the content of MDA was measured by thiobarbituric acid method, the content of CAT was measured by visible light method, and the content of NO was measured by nitrate reductase method.

[0031] Results: Compared with the control group, the SOD activity in the liver tissue of the model groups with injection doses of 125mg / kg and 150mg / kg was significantly reduced (P<0.01),...

Embodiment 3

[0040] Example 3 Detection of model apoptotic cells built by the present invention

[0041]TUNEL method (TdT-mediated dUTP nick-end labeling method) was used to analyze the apoptosis of the model established in Example 1 of the present invention: a part of the liver tissue was taken to prepare paraffin sections, and the paraffin sections were conventionally dewaxed and hydrated. Using the American R&D company peroxidase kit, labeled with fluorescein, then combined with peroxidase anti-fluorescein antibody and fluorescein, and then reacted with the substrate DAB for color development. Read the slides under an optical microscope and take pictures. A large number of apoptotic hepatocytes ( image 3 ). This shows that the nano-zinc oxide suspension with a concentration of 125mg / kg and above can obviously induce the apoptosis of liver cells in animals.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a building method of a mouse liver apoptosis animal model induced by nanometer zinc oxide.The method comprises the steps that the nanometer zinc oxide is suspended in a buffer solution to be prepared into a nanometer zinc oxide suspension, the nanometer zinc oxide suspension is intraperitoneally injected into a mouse once a day for 30 days continuously, and the mouse liver apoptosis animal model induced by the nanometer zinc oxide is successfully built; by mass of the nanometer zinc oxide, the injection amount of the nanometer zinc oxide suspension is 50-150 mg / kg.The built animal model has the advantages of being easy to prepare, stable, reliable, repeatable and the like.The model provides a new path and thought for deeply researching heavy metal poisoning pathogenesis and medicine development.

Description

(1) Technical field [0001] The invention relates to the establishment of an animal model, in particular to a method for establishing an animal model of mouse liver cell apoptosis induced by nanometer zinc oxide. (2) Background technology [0002] With the development of nanotechnology, more and more nanomaterials have entered people's lives. Nanoparticles are widely used in medicine and chemical industry due to their small size effect, surface effect, quantum size effect and macroscopic quantum tunneling effect. However, nanoparticles have their special biological activity, which also makes them have some special toxicity. More and more studies are focusing on the application of nanomaterials, but also on the potential impact of nanoparticles on human health and the environment. [0003] Compared with ordinary zinc, nano zinc oxide has better performance in many aspects such as electricity, optics, national defense, textile and feed industry, and has been widely used in man...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 庄海峰林婉冰沈建平陈峥雷澍庄晓芬
Owner ZHEJIANG PROVINCIAL HOSPITAL OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com