Method for detecting content of cyanide in milk and milk powder
A detection method and cyanide technology, which are applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as interference in cyanide determination, no reports on cyanide detection methods, etc., and achieve good stratification, accurate and reliable determination, and low detection. out-of-limit effect
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[0063] Example 1: Determination of cyanide content in commercially available milk and milk powder.
[0064] (1) Sample collection and preparation:
[0065] Buy 10 different brands or different types of milk (including breakfast milk, high-calcium milk, pure milk, etc.) and 10 milk powders (whole milk powder, skimmed milk powder, infant milk powder, etc.) from the supermarket. Milk powder samples were diluted with deionized water at a ratio of 1:10 (m / v), mixed, and then processed and tested.
[0066] (2) Sample pretreatment:
[0067] Take 2.0mL of the above milk or milk powder dilution, add an appropriate amount of 0.1mol / L potassium hydroxide solution to adjust its pH to about 10, add 400μL of 0.05mol / L octadecyltrimethylammonium bromide solution and 2.0mL Pentafluorobenzyl bromide-dichloromethane (0.4%, v / v) solution, vortexed on a vortex mixer for 2min, then derivatized in a water bath at 30°C for 30min, and then derivatized at 10000r / min at 4°C Centrifuge at a rotationa...
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