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Molecular imprinting and preparation method thereof

A molecular imprinting and template molecule technology, applied in the field of preparation of the molecular imprinting, can solve the problems of restricting the application of molecular imprinting technology, and achieve the effects of good application prospect, low production cost and process safety.

Inactive Publication Date: 2016-06-22
HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the substances used to prepare molecularly imprinted polymers in the prior art belong to chemical polymer materials. In the development and research of biomaterials such as chitosan, chemical substances are also used as modifiers and crosslinking agents. Therefore, the application of molecular imprinting technology in areas with high biological requirements is limited.

Method used

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  • Molecular imprinting and preparation method thereof
  • Molecular imprinting and preparation method thereof
  • Molecular imprinting and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This example concerns a set of molecular imprints and their preparation.

[0032] The preparation of molecular imprints uses the following steps:

[0033] a. At 25°C and 1 standard atmospheric pressure, add chitosan (CS) into 20mL of acetic acid solution with a mass content of 2%, stir well until completely dissolved, then add polyethylene glycol 6000 with a mass fraction of 3%. (PEG6000), stirring continuously for 30 minutes to make it evenly mixed, then adding pectin (PT) and template molecules, continuing to stir for 20 minutes, fully reacting to obtain a viscous solution;

[0034] b. Place the viscous solution obtained in step a on a watch glass until it reaches a flat surface, place it in a blast drying oven at 42±1°C to make it fully dry, and soak the dried polymer in deionized water for 12 hours. The template molecule is eluted to obtain a molecular imprint.

[0035] Molecular imprinting was prepared using the amounts of each substance (including PT, CS, PEG600...

Embodiment 2

[0039] This example relates to the verification of the adsorption performance of the molecular imprint of the present invention on template molecules.

[0040] In this embodiment, the adsorption performance to the template molecule is reflected by the adsorption amount Q, and the determination method of the Q value is:

[0041] Put the substance to be tested in the template molecule solution, and after reaching the adsorption equilibrium, use a centrifuge to separate the solution, take the supernatant, and measure the absorbance with an ultraviolet spectrophotometer, and calculate its concentration through the standard curve of the template molecule, namely C1, according to The molecular weight M of the template molecule, and the adsorption amount Q are calculated by the following formula.

[0042] Q = ( C 0 - C 1 ...

Embodiment 21

[0052] This example relates to the effect of the dosage ratio of chitosan and pectin on the molecular properties of molecularly imprinted adsorption templates. Detect the adsorption amount Q of molecular imprinted 5-sulfosalicylic acid obtained in Examples 1.1 to 1.3, as shown in the following table:

[0053]

[0054] Example 1.1(0) in Example 2.1.1 in the table represents the molecularly imprinted material of Example 1.1, that is, the material, amount of material and preparation method are basically the same as those in Example 1.1, the only difference is that no template is added during the preparation process Molecule 5-sulfosalicylic acid; the selection factor a is the ratio of the adsorption capacity of Example 1.1 and Example 1.1 (0), and is used to characterize the selective adsorption performance. Similarly, Examples 1.2(0) and 1.3(0) respectively represent the materials of Examples 1.2 and 1.3.

[0055] It can be seen from the results in the above table that the m...

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Abstract

The invention provides molecular imprinting. The material of the molecular imprinting is prepared from chitosan and pectin, and one of 5-sulfosalicylic acid, L-tyrosine or R-tyrosine is used as a template molecule. The obtained molecular imprinting has good biocompatibility and biodegradability and low toxicity, is stable for hot solvent, organic solvent and strong acid and strong alkaline solvent, has the performance for resisting the adverse environment, has the advantage of being long in service life, and can meet requirements of the fields having high requirements for toxicity, degradability and the like of biological materials. The preparation process of the molecular imprinting is simple, the molecular imprinting is prepared only by conducting cross-linking reaction on chitosan and pectin in water without using modifier, cross-linking agents or other substances, the production cost is low, the process is safe, and the molecular imprinting is suitable for large-scale industrialized application.

Description

technical field [0001] The invention relates to the technical field of molecular imprinting, in particular to a molecular imprinting, and at the same time, the invention also relates to a preparation method of the molecular imprinting. Background technique [0002] Molecular imprinting technology is a technique for synthesizing molecularly imprinted polymer (also called molecularly imprinted) materials with molecular recognition function. The principle of molecular imprinting is that when template molecules (or imprinted molecules) come into contact with polymer material monomers, multiple interaction points will be formed. , this effect is memorized through the polymerization process. When the template molecule is removed, a cavity with multiple action points matching the spatial configuration of the template molecule is formed in the polymer material. Its analogs have selective recognition properties, which are vividly described as "artificial locks" that recognize "molecu...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L5/08C08L5/06
CPCC08J3/246C08J2305/08C08J2405/06C08L5/08C08L2201/06C08L5/06
Inventor 郑学芳廉琪贾丹丹王东军
Owner HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
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