Method for preparing environment-friendly alpha-arbutin by using bioenzyme reaction and water crystallization purification

An environmentally friendly and arbutin technology, applied in fermentation and other directions, can solve the problems of difficulty in ensuring the complete removal of organic solvents, large safety hazards, and low efficiency, and achieve low cost, low allergenicity, and good stability.

Inactive Publication Date: 2016-06-15
广东乐尔康生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, they introduce foreign enzymes in the production process, which is prone to by-products and unpredictable hazards
[0004] There are also many related reports on the separation and purification process of α-arbutin. Patent CN104911238A discloses the purification of α-arbutin fermentation liquid by nanofiltration method. - For arbutin, it is difficult to remove the sugar in it
Patent CN103773827A discloses the purification of α-arbutin fermentation liquid by macroporous resin adsorption method, but because it involves processes such as adsorption, washing impurities, elution and regeneration, the whole process is more complicated, time-consuming and not high in efficiency
In the α-arbutin method of chemical synthesis, there are also some methods for purification by adding organic solvents to crystallize the product, but it is difficult to ensure that the organic solvents are completely removed, and the production process poses a greater safety hazard

Method used

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  • Method for preparing environment-friendly alpha-arbutin by using bioenzyme reaction and water crystallization purification
  • Method for preparing environment-friendly alpha-arbutin by using bioenzyme reaction and water crystallization purification
  • Method for preparing environment-friendly alpha-arbutin by using bioenzyme reaction and water crystallization purification

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Experimental program
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Effect test

Embodiment 1

[0038] A method for preparing environmentally friendly α-arbutin by using biological enzyme reaction and water crystallization purification includes biological enzyme synthesis of α-arbutin and water crystallization separation and purification of α-arbutin; biological enzyme synthesis of α-arbutin enzyme The components of the reaction bottom solution (in total weight percentage) are 5.0%~15.0% of disaccharide, 0.5%~5.0% of 1,4-hydroquinone, 0.02%~0.08% of sodium hydroxide, and 0.1%~2.0% of auxiliary agent and the remaining amount of purified water; add 5-50g / L α-arbutinase to the enzyme reaction bottom solution, and react at 30-50°C to obtain the initial product solution of α-arbutin.

[0039] The biological enzyme synthesis α-arbutin is prepared by the following method:

[0040] (1) Weigh 10.0% disaccharide and 1.5% auxiliary agent, add purified water and stir to dissolve;

[0041] (2) Add sodium hydroxide to adjust the pH value to 6.5, then add 1.0% 1,4-hydroquinone, stir a...

Embodiment 2

[0054] A method for preparing environmentally friendly α-arbutin by using biological enzyme reaction and water crystallization purification, including biological enzyme synthesis of α-arbutin and water crystallization separation and purification of α-arbutin, biological enzyme synthesis of α-arbutin The components of the enzyme reaction bottom liquid (in total weight percentage) are 5.0%~15.0% of disaccharide, 0.5%~5.0% of 1,4-hydroquinone, 0.02%~0.08% of sodium hydroxide, and 0.1%~2.0% of auxiliary agent % and the rest of purified water, add 5-50g / L α-arbutinase to this enzyme reaction bottom solution, and react at 30-50°C to obtain the initial product solution of α-arbutin.

[0055] The biological enzyme synthesis α-arbutin is prepared by the following method:

[0056] (1) Weigh 8.0% disaccharide and 1.2% auxiliary agent, add purified water and stir to dissolve;

[0057] (2) Add sodium hydroxide to adjust the pH value to 6.0, then add 1.0% 1,4-hydroquinone, stir to dissolve...

Embodiment 3

[0070] A method for preparing environmentally friendly α-arbutin by using biological enzyme reaction and water crystallization purification includes biological enzyme synthesis of α-arbutin and water crystallization to separate and purify α-arbutin, and biological enzyme synthesis of α-arbutin enzyme The components of the reaction bottom solution (in total weight percentage) are 5.0%~15.0% of disaccharide, 0.5%~5.0% of 1,4-hydroquinone, 0.02%~0.08% of sodium hydroxide, and 0.1%~2.0% of auxiliary agent And the remaining amount of purified water, add 5-50g / L α-arbutinase to the enzyme reaction base solution, and react at 30-50°C to obtain the initial product solution of α-arbutin.

[0071] The biological enzyme synthesis α-arbutin is prepared by the following method:

[0072] (1) Weigh 10.0% disaccharide and 1.5% auxiliary agent, add purified water and stir to dissolve;

[0073] (2) Add sodium hydroxide to adjust the pH value to 6.5, then add 1.0% 1,4-hydroquinone, stir and dis...

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Abstract

The invention relates to a method for preparing environment-friendly alpha-arbutin by using bioenzyme reaction and water crystallization purification. The preparation method of the environment-friendly alpha-arbutin comprises the following steps: bioenzyme-based alpha-arbutin synthesis: adding a 5-50 g/L alpha-arbutin enzyme into an enzyme reaction base solution, and reacting at 30-50 DEG C to obtain an alpha-arbutin primary product solution, wherein the enzyme reaction base solution for bioenzyme-based alpha-arbutin synthesis is composed of 5.0-15.0 wt% of disaccharide, 0.5-5.0 wt% of 1,4-hydroquinone, 0.02-0.08 wt% of sodium hydroxide, 0.1-2.0 wt% of aid and the balance of purified water; and purifying the alpha-arbutin primary product by water crystallization separation 3-7 times, thereby obtaining the environment-friendly alpha-arbutin. The method for preparing alpha-arbutin has the advantages of simple technique, high safety and low cost, and is suitable for mass production. The obtained alpha-arbutin product is environment-friendly, has the advantages of high purity, high stability and low sensitization, and can be used for various whitening products.

Description

technical field [0001] The invention relates to the technical field of cosmetic raw materials, and relates to the preparation of glycosides, in particular to a method for preparing environmentally friendly α-arbutin by utilizing biological enzyme reaction and water crystallization purification. Background technique [0002] α-Arbutin exists in plants such as bearberry and lingonberry, which can effectively inhibit the activity of tyrosinase in the skin and reduce the deposition of tyrosinase in the skin. It is a non-irritating, non-allergic and compatible Strong natural whitening active substance, which has bleaching, anti-pigmentation and anti-freckle effects on the skin. [0003] In the production process of α-arbutin, the preparation method and separation and purification technology play a vital role in the final quality of the product. At present, there are many methods for preparing α-arbutin. The more efficient and green production processes include fermentation metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44
CPCC12P19/44
Inventor 周银泉
Owner 广东乐尔康生物科技股份有限公司
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