Method for producing inonotus obliquus powder through rapid and deep liquid state fermentation

Abstract

The invention discloses a method for producing Inonotus obliquus bacterial powder by rapid deep liquid fermentation. The method is to quickly obtain a fermented liquid rich in a large amount of mycelium by adopting a semi-continuous fermentation process for the Inonotus obliquus strain, and then The fermented liquid is treated and separated by a microfiltration membrane to obtain the mycelia of Inonotus obliquus. The invention changes the traditional intermittent batch fermentation process of Inonotus obliquus, and the separation of mycelium adopts efficient ceramic membrane microfiltration to replace the centrifuge separation mode. The semi-continuous fermentation process overcomes the shortcomings of the traditional batch fermentation process, such as many process links, long intermediate intervals, and low equipment efficiency; and the use of high-efficiency ceramic membranes to filter the fermentation liquid can reduce the mycelium entering the filtrate, which can not only improve the bacterial The yield of silk can reduce the COD of the filtrate, reduce the amount of sewage treatment, without destroying the cells themselves, and ensure the recovery of all active ingredients in the cells. Compared with the traditional technology, the invention has remarkable economic benefit, social benefit and environmental protection benefit.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for rapidly producing fungal mycelium by deep liquid fermentation technology, more precisely, a method for rapidly producing Inonotus obliquus powder by deep liquid fermentation. Background technique [0002] Microbial fermentation can be divided into batch, fed-batch, semi-continuous, continuous and other modes. Batch fermentation consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the fermentation cycle is long, and the production efficiency is low; Fed-batch fermentation Although the lack of nutrients or precursors can be supplemented by feeding, the product synthesis will inevitably be blocked due to the continuous accumulation of harmful metabolites; the production intensity of continuous fermentation is greatly improved compared wit...

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Problems solved by technology

[0006] The traditional liquid fermentation process of Inonotus obliquus is mostly an intermittent batch fermentation process, but the batch fermentation process consumes a lot of manpower and material resources, and each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, cleaned, etc. The operation is complicated, the process is cumbersome, the non-fermentation period is long, and the production efficiency is low; while the subsequent separation process is traditionally separated by a centrifuge. This method is subject to the influence of the separation factor of the centrifuge, and the mycelium is subjected to shear force during the separation process. Destruction, part of the active ingredients are lost into the filtrate, resulting in a decrease in product quality and yield. The separated filtrate is turbid and contains more broken mycelia, resulting in a high COD of the discharged filtrate. If the filtrate meets the sewage discharge standard, it needs to pass through Complex sewage treatment, the workload of the sewage treatment system is too large, which brings a serious economic burden to the enterprise

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