Preparation for inhibiting poxvirus infections

A pox virus and preparation technology, applied in the field of preparations for inhibiting pox virus infection, can solve the problems of epidemic spread, difficult prevention and control effects of specific prevention and treatment drugs, and no curative effect, and achieve the effect of inhibiting infection

Active Publication Date: 2016-06-15
INST OF PLA FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The reality and potential threats of viral infectious diseases are becoming more and more serious for human beings, and the pressure on the prevention and control of sudden infectious diseases is increasing. When the epidemic broke out, the original specific prevention and treatment drugs were difficult to exert their due prevention and control effects, or even had no curative effect, and the limitations of human cogni...

Method used

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  • Preparation for inhibiting poxvirus infections
  • Preparation for inhibiting poxvirus infections
  • Preparation for inhibiting poxvirus infections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of plant extract compound formula virus infection inhibitor

[0039] Epigallocatechin gallate (EGCG), tannic acid and astragalus polysaccharide are uniformly mixed according to a mass ratio of 1:1:1.5 to obtain a plant extract compound formulation.

[0040] Before the experiment, it was dissolved in PBS buffer, made into 2560 μg / ml (the total concentration of EGCG, tannic acid and astragalus polysaccharide in the solution), filtered, and after sterilization, it was stored at -20°C for later use.

Embodiment 2

[0041] Embodiment 2, cytotoxicity test

[0042] In this example, the neutral red phagocytosis method was used to measure the cytotoxicity of the compound formulation prepared in Example 1 to mammalian cells. The specific operation is as follows:

[0043] The compound formulation preparation solution (2560 μg / ml) prepared in Example 1 was diluted step by step to obtain a total of 6 concentration. Then the dilutions of different dilutions were added to the 96-well cell culture plate with Vero cells and the cell density was about 80%, 100 μl in each well, and 4 duplicate holes were made for each dilution. Add compound formula preparation) as a control, after 2 hours of action, discard the test solution, add cell maintenance culture medium, add 200 μ l to each well, place it in a cell incubator and cultivate, after 48 hours, add 0.1% (0.1g / 100mL) to each well Neutral red 25μl, after reacting at 37°C for 1.5h, suck out the liquid in each well and rinse twice with PBS. Add 100 μ...

Embodiment 3

[0048] Embodiment 3, pox virus infection inhibition test

[0049] In this example, the CPE method was used to measure the inhibitory effect of the compound formulation prepared in Example 1 on poxvirus infection. The tested poxvirus was vaccinia virus WR strain.

[0050] Take a 96-well culture plate cultured with Vero cells that has grown into a single layer of cells with a growth density of about 80%, pour off the culture medium, rinse the cells with PBS for 3 times, and add them under the three conditions of A, B, and C respectively. The compound formula preparation that example 1 prepares:

[0051] A. Simultaneously with virus adsorption: put an equal volume of 2×100TCID 50 After mixing the poxvirus virus solution with 2 times the concentration of the test substance solution, add 100 μl / well of the mixed solution to the cell culture plate, place it in the cell culture incubator, and discard it after the virus is adsorbed for 1 hour. After washing the cell surface 3 times...

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Abstract

The invention discloses a preparation for inhibiting poxvirus infections and provides an application of the preparation to the following example (1) or (2): (1) preparation of a product for inhibiting the poxvirus infections; (2) inhibition of the poxvirus infections; the preparation is mainly prepared by mixing epigallocatechin gallate, tannin and astragalus polysaccharides in the mass ratio being (0.5-1.0):(0.5-1.0):(0.5-1.5). The cytotoxicity of the compound formula preparation is not higher than that of control sample ribavirin already having the security permission, and the preparation is safer. Under the condition of safe use concentration, the poxvirus infections can be effectively inhibited by preventively using the preparation, and the preparation has the commercial value in being further developed into a poxvirus infection inhibitor.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a preparation for inhibiting poxvirus infection. Background technique [0002] Poxviruses are a large family that can infect both humans and animals, often causing local or systemic purulent skin lesions in infected persons, and can lead to outbreaks of infectious diseases. Of particular note is the smallpox virus, which reproduces rapidly and can be transmitted through the air at an extremely rapid rate. Patients with the virus are most contagious within 1 week of infection because their saliva contains the largest amount of smallpox virus, until After the patient's scar is peeled off, the smallpox virus can also be transmitted through the patient, which has led to many global outbreaks in human history. In the case of malignant infection, the smallpox virus causes serious diffuse damage in the tissue layer or deep layer of the skin, and a large amount of blood in the patient's body f...

Claims

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Application Information

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IPC IPC(8): A61K36/481A61K31/715A61P31/20A61K31/353A61K31/7024
Inventor 常国辉刘京梅杨益黄留玉罗丽晓李瑾惠罗彦军孙走南唐玥苏文莉张洁刘璇
Owner INST OF PLA FOR DISEASE CONTROL & PREVENTION
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