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Silkworm chrysalis small molecule peptide chelate for improving immunity and preparation method thereof

A small peptide chelate and immunity technology, applied in the biological field, can solve the problems of low chromium utilization rate and limited utilization, and achieve the effects of low cost, small toxic and side effects, and easy preparation

Inactive Publication Date: 2016-06-15
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, the utilization rate of chromium is relatively low, which limits its utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Homogenize the silkworm chrysalis with 10 times the amount of water, heat and boil for 15 minutes, wait to cool to about 40°C, adjust the pH value to 2.0, add 1% pepsin, keep warm for 1 hour, adjust the pH value to 8.0, add 1% trypsin, and keep warm for 3 hour, boiled for 15 minutes, centrifuged, the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, and the permeate was added to a DA201-C macroporous adsorption resin column, eluted with water, and the water wash was discarded, and then Elute with 90% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a clear paste with a relative density of 1.10-1.15 at 60°C, spray dry (inlet air temperature is 180-200°C, outlet air temperature is 90 ~100°C) to obtain silkworm chrysalis small peptide extract.

[0059] Dissolve chromium nicotinate in distilled water to make a salt solution with a concentration of 15 mg / ml, adjus...

Embodiment 2

[0061] Homogenize the silkworm chrysalis with 12 times the amount of water, heat and boil for 30 minutes, wait to cool to about 45°C, adjust the pH value to 1.5, add 2% pepsin, keep warm for 2 hours, adjust the pH value to 9.0, add 2% trypsin, and keep warm for 4 hour, boiled for 15 minutes, centrifuged, the supernatant was ultrafiltered through an ultrafiltration membrane with a relative molecular mass of 3KD, and the permeate was added to a DA201-C macroporous adsorption resin column, eluted with water, and the water wash was discarded, and then Elute with 75% ethanol, collect the ethanol eluate, recover ethanol under reduced pressure and continue to concentrate to a clear paste with a relative density of 1.10-1.15 at 60°C, spray dry (inlet air temperature is 180-200°C, outlet air temperature is 90 ~100°C) to obtain silkworm chrysalis small peptide extract.

[0062] Dissolve zinc gluconate in distilled water to make a salt solution with a concentration of 10 mg / ml, adjust th...

Embodiment 3

[0064] Get the silkworm chrysalis small peptide chelate 100g that embodiment 1 method gained, by silkworm chrysalis small peptide chelate: corn oil: beeswax: lecithin ratio is 10:36:3:1 mixed material, through colloid mill grinding 30 minutes, with Gelatin, glycerin, purified water, and iron oxide black are used to make soft capsule skin glue at a ratio of 100:40:100:1. The temperature of the plastic box is controlled at 55±5°C, the temperature of the heat preservation tank is 40±5°C, and the temperature of the spray body is 36±5°C. , press soft capsules with a pill press, the content of each capsule weighs 0.50g, dry in cold air at 18°C ​​to 26°C for 4 hours (relative humidity is 30% to 40%) to shape, wash, dry, select pills, pack, and prepare into soft capsules. Capsule finished product.

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PUM

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Abstract

The present invention discloses a silkworm chrysalis small molecule peptide chelate for improving immunity and a preparation method thereof, and belongs to the field of biotechnology. The chelate is characterized in that the chelate is prepared by using the silkworm chrysalis as the starting material, and performing enzymolysis, ultrafiltration, resin column purification, chelating reaction and drying, and corresponding preparation can be obtained according to the conventional method with the addition of pharmaceutical excipients. The chelate can significantly improve immunity, and has no toxic side effect, no drug-induced injury, low cost and other advantages.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a silkworm chrysalis small peptide chelate compound for improving immunity and a preparation method thereof. Background technique [0002] Immunity refers to the ability of the body to resist external invasion and maintain the stability of the internal environment. It is the body's own defense mechanism. Self cells and the ability to recognize and process mutant cells and virus-infected cells in the body. Modern immunology believes that improving immunity is the physiological response of the human body to recognize and eliminate "others", and the immune system performs this function in the human body. For millions of years, human beings have lived in an environment that is both suitable for survival and full of dangers. Human beings have survived and acquired extraordinary immunity. Therefore, immunity is the product of the biological evolution process. For various reasons, the imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23L33/165
CPCA23V2002/00A23V2200/324A23V2200/30
Inventor 刘国飞代龙凌中鑫
Owner BINZHOU MEDICAL COLLEGE
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