Method for extracting beta-carotene from Dunaliella salina by utilizing subcritical fluid

Abstract

The invention provides a method for extracting beta-carotene from Dunaliella salina by utilizing subcritical fluid. The method comprises the following steps: I, processing the Dunaliella salina to prepare Dunaliella salina granules; II, fill kettle with granules the Dunaliella salina granules into an extraction kettle, and introducing the subcritical fluid into the extraction kettle; then performing heating extraction under stirring to obtain extract and an extracted solid; III, introducing the extract into a separation kettle from the extraction kettle for decompression separation to obtain beta-carotene extractum. According to the method provided by the invention, a subcritical fluid extraction method is applied to extracting the beta-carotene of the Dunaliella salina, oxidation loss of the natural beta-carotene is reduced by low-temperature continuous counterflow extraction and low-temperature separation, and the yield is greatly increased; moreover, the safety problem of solvent residue is solved by the low-temperature separation, meanwhile, extraction cost is greatly reduced, and industrial production is easy; a brand-new way is opened for extracting the natural beta-carotene extractum from the Dunaliella salina by the method provided by the invention.

Description

technical field [0001] The invention relates to the technical field of extraction methods of beta-carotene, in particular to a method for extracting beta-carotene from Dunaliella salina by using a subcritical fluid. Background technique [0002] Dunaliella salina, belonging to Chlorophyta, Volvox, and Dunaliella genus, is a single-celled algae that has lived on the earth for 3.8 billion years. Usually oval, 8-25 μm long, 5-15 μm wide, with two long flagella of 2-15 μm, movable. It can adapt to low-concentration brine to saturated brine (35%-36%), so it is widely distributed in natural salt lakes and salt pans in the United States, Australia, Israel, China and other countries. When the concentration accumulates to a certain level, the lake or salt pan will appear red and pink. [0003] Under the conditions of strong photosynthesis and high salinity, Dunaliella salina accumulates a variety of nutrients needed for human health, including a variety of natural carotenoids, foli...

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned defects and problems of the prior art, the purpose of the present invention is to provide a method for extracting β-carotene from Dunaliella salina by using subcritical fluid, so as to solve the problem that the existing extraction methods will bring β-carotene leaching. Oxidation and decomposition of the paste, resulting in low extraction rate of β-carotene; and technical problems of poor quality of β-carotene extract due to solvent residue