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Extraction method of phyllanthus emblica polyphenol

An extraction method and technology of Emblica emblica are applied in the field of extraction of polyphenols from Emblica Fructus, can solve the problems of high oxidation loss of polyphenols, low product purity, low extraction rate, etc., and achieve the advantages of low extraction temperature, improved purity, and reduced oxidation loss. Effect

Inactive Publication Date: 2014-05-07
HEFEI KANGLING HEALTH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main active ingredient of the efficacy of Amla emblica is polyphenols from emblica emblica. The existing public extraction methods for polyphenols from emblica emblica mostly use the traditional hot-dip extraction method, but the above-mentioned method has high polyphenol oxidation loss, low extraction rate, and high product purity. Low disadvantages, unable to meet the needs of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Take 500g of fresh emblica fruit, remove the stem, wash it with deionized water, control the water, and remove the core;

[0017] (2) Take an 80% acetone solution equivalent to twice the weight of the fresh fruit of Amla emblica and pre-cool it to 4°C, then mix the above-mentioned pretreated emblica pulp with pre-cooled acetone, add it to a tissue masher, After mashing for 3 minutes, transfer to a high-speed homogenizer, and homogenize for 6 minutes in an ice bath at 4°C at a speed of 2000r / min;

[0018] (3) Place the homogenate obtained above in a magnetic oscillator to vibrate for 0.5h at a vibration frequency of 4000r / min, then centrifuge at a temperature of 4°C and a speed of 10000r / min for 12min, take the supernatant, and repeat the above for the precipitate The step is extracted twice, the supernatants are combined, acetone is recovered, and the total extract is obtained;

[0019] (4) Take a hollow fiber membrane with a pore size of 0.1 μm and...

Embodiment 2

[0023] (1) Take 500g of fresh emblica fruit, remove the stem, wash it with deionized water, control the water, and remove the core;

[0024] (2) Take a 70% acetone solution equivalent to 4 times the weight of the fresh fruit of Amla emblica and pre-cool it to 2°C, then mix the above-mentioned pretreated emblica pulp with pre-cooled acetone, add it to a tissue masher, After mashing for 5 minutes, transfer to a high-speed homogenizer, and homogenize for 4 minutes in an ice bath at 2°C at a speed of 3000 r / min;

[0025] (3) Place the homogenate obtained above in a magnetic oscillator to vibrate for 1 hour at a vibration frequency of 3000r / min, then centrifuge at a temperature of 2°C and a speed of 15000r / min for 8 minutes, take the supernatant, and repeat the above steps for the precipitate Extracted 3 times, combined the supernatant, recovered acetone, and obtained the total extract;

[0026] (4) Take a hollow fiber membrane with a pore size of 0.2 μm and soak i...

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Abstract

The invention discloses an extraction method of phyllanthus emblica polyphenol. The extraction method comprises the following steps: removing stalks of obtained phyllanthus emblica fresh fruit, cleaning with deionized water, draining water, removing kernels, adding the phyllanthus emblica fresh fruit to a precooling acetone solution, pounding, homogenizing, carrying out magnetic force oscillation, centrifuging, taking supernatant, repeating the steps so as to extract precipitates for two to three times, combining supernatant, and recycling acetone, thus obtaining a total extracting solution; filtering the total extracting solution by using a pretreated hollow fibrous membrane; purifying a hollow fibrous membrane filtering medium by using a polyamide chromatographic column, collecting eluant, recycling alcohol, concentrating, and carrying out freeze drying, thus obtaining the phyllanthus emblica polyphenol. Compared with a conventional heat extraction method, the extraction method disclosed by the invention has the advantages that the extraction temperature is low, a technology is simple and easy to operate, the extraction effect is good, the extraction rate of the phyllanthus emblica polyphenol reaches above 95%, and the purity of the phyllanthus emblica polyphenol finished product reaches above 99%.

Description

technical field [0001] The invention relates to a method for extracting emblica polyphenols, belonging to the technical field of extraction of traditional Chinese medicine components. Background technique [0002] Phyllanthus emblica belongs to Euphorbiaceae Phyllostachys genus, its fresh fruit is sweet and sour, crisp and slightly astringent, with a sweet aftertaste, hence the name Emlical emblica, also known as Houganzi, An Luoguo, Niuganguo, etc. The fruit of Amlican emblica is sour and slightly astringent, clears heat and cools blood, promotes digestion and invigorates the spleen, promotes body fluid and quenches thirst. Indications for blood heat and blood stasis, indigestion, abdominal distension, cough, sore throat, dry mouth and vitamin C deficiency. In Tibetan medicine, emblica mainly treats Bacon's disease, Chiba disease, blood disease, hypertension and so on. Research results in recent years have shown that emblica has anti-inflammatory, anti-oxidation, a...

Claims

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Application Information

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IPC IPC(8): A61K36/47A61K131/00
Inventor 陈杏爱
Owner HEFEI KANGLING HEALTH TECH
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