A kind of tissue culture rapid propagation method of Ribbon grass

Abstract

The invention discloses a tissue culture and rapid propagation method of ribbon grass. The tissue culture and rapid propagation method of the ribbon grass comprises the following steps of cleaning up underyearling and tender stem sections of the ribbon grass; obtaining an explant after sterilization treatment by adopting alcohol and mercury bichloride; inoculating the explant onto a starting medium to culture, and cutting off an axillary bud obtained through culturing and inoculating onto an initial medium to culture; taking a ribbon grass seedling obtained through culturing as a female parent, cutting, then inoculating onto a subculture medium to culture until growing to a complete plant, wherein the starting medium, the initial medium and the subculture medium are formed by 1 / 2 of DKW minimal medium, supplement of 0.5 to 1.0mg / L of TDZ, 0.06 to 0.15mg / L of NAA, 10 to 50 g / L of cane sugar, and 2 to 10 g / L of agar; finally washing away the culture medium from the obtained grew well tissue culture rooting seedling, and then directly transplanting into a greenhouseseed bed to propagate. The tissue culture and rapid propagation method provided by the invention is adopted to propagate the ribbon grass, is simple and convenient in culture process, and simple and easy to operate, and has the characteristics of high propagation coefficient, short propagation period and high survival rate.

Description

technical field [0001] The invention relates to the technical field of plant asexual propagation, in particular to a tissue culture and rapid propagation method of Ribbon grass. Background technique [0002] "Ribbon Grass" alias: jade belt grass Latin name: Phalaris arundinacea L.var.picta L. is a variant of Phalaris arundinacea L.var.picta L., a perennial herb of the Gramineae family. Most of the stems are solitary, and some are clustered. There are white stripes in between, soft and ribbon-like, hence the name, the panicle is tight and narrow, the branches are straight up, dense spikelets, glabrous or microhair, the glumes are rough along the ridge, and the upper part has very narrow wings . It is an excellent pasture for livestock when young, and has strong regeneration ability after harvesting or grazing. The stalks can be used for weaving utensils or papermaking. It can be used to decorate landscape belts such as river embankments and wetlands, and has great ornamenta...

AI Technical Summary

Problems solved by technology

[0003] At present, ribbon grass is mainly propagated by sowing and branching, but the reproductive rate is low, which is far from meeting the domestic demand for seedlings

At the same time, the current conventional propagation method of Ribbon grass has a long breeding cycle, which is not conducive to the large-scale production, development and application of Ribbon grass.