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Method for quickly measuring content of vitamin C in Fructus lycii

A technology for rapid determination of vitamins, applied in measurement devices, chemical method analysis, chemical analysis by titration, etc., can solve the problems of large equipment investment, long time, complicated operation, etc. simple effect

Inactive Publication Date: 2016-06-01
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention aims to solve the technical problems that the current determination method of vitamin C in Chinese wolfberry needs not only to decolorize or oxidize the sample liquid, but also to prepare many medicines, require large equipment investment, complicated operation, and long time. Method for Rapid Determination of Vitamin C Content in Lycium barbarum

Method used

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  • Method for quickly measuring content of vitamin C in Fructus lycii
  • Method for quickly measuring content of vitamin C in Fructus lycii
  • Method for quickly measuring content of vitamin C in Fructus lycii

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specific Embodiment approach 1

[0024] Specific implementation mode one: this implementation mode is a kind of method for fast determination vitamin C content in wolfberry, specifically is carried out according to the following steps:

[0025] 1. Prepare the sample to be tested: put 1g~2g of wolfberry to be tested into a mortar, add acid solution until the wolfberry to be tested is completely immersed in the acid solution, grind the wolfberry to be tested into a homogeneous slurry, and transfer the homogenate into In a 100mL volumetric flask, wash the mortar with an acid solution and pour the washing liquid into a 100mL volumetric flask, then use the acid solution to make up the volume to the mark, shake well and let stand for 10min, filter to obtain the filtrate;

[0026] 2. Acid-base neutralization titration: Take 10mL of the filtrate from step 1 and place it in a 250mL conical flask, add 50mL-100mL of deionized water, add 3 drops of 0.5% phenolphthalein solution as an indicator, and use standard hydrogen ...

specific Embodiment approach 2

[0040] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the configuration method of the hydrochloric acid aqueous solution described in step one is: get 9.4mL mass fraction with pipette and be that the hydrochloric acid of 37.5% is put into 1000mL volumetric flask , add water to dilute, and set the volume to the mark to obtain a hydrochloric acid aqueous solution with a concentration of 0.1mol / L, and then dilute according to actual needs. Others are the same as the first embodiment.

specific Embodiment approach 3

[0041] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the preparation method of the standard sodium hydroxide solution described in step 2 is: the NaOH that takes 4g is dissolved in deionized water in a small beaker, Then transfer it into a 1000mL volumetric flask, add water to dilute to the mark, calibrate with potassium hydrogen phthalate to obtain a standard sodium hydroxide solution, and then dilute it according to actual needs. Others are the same as those in Embodiment 1 or 2.

[0042] Prove the beneficial effect of the present invention by following test:

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Abstract

The invention relates to a method for measuring content of vitamin C in Fructus lycii, in particular to a method for quickly measuring content of vitamin C in Fructus lycii, and aims to solve the technical problem that decoloring or oxidizing needs to be performed on sample liquid and the technical problems of need for preparing many drugs, large instrument-equipment investment, complex operation and high time consumption. The method includes: 1, preparing a to-be-measured sample; 2, performing acid-base neutralization titration; 3, titrating for a blank test; 4, calculating content of vitamin C in Fructus lycii. The method has the advantages that decoloring or oxidizing does not need to be performed on colored sample liquid to be measured, the method has the advantages of small investment, low reagent consumption, simple operation, high stability and wide application range and is suitable for quickly measuring content of vitamin C in Fructus lycii, and the measuring range can be conveniently adjusted by increasing filtrate sampling amount or increasing sample liquid diluting ratio. The method is applied to measuring content of vitamin C in Fructus lycii.

Description

technical field [0001] The invention relates to a method for measuring vitamin C content in wolfberry. Background technique [0002] Vitamin C (Vc), chemical name is ascorbic acid, hexuronic acid, molecular formula is C 6 h 8 o 6 , the molecular weight is 176.13, the pure product is white crystal or crystalline powder, odorless and sour. Vitamin C is a water-soluble vitamin, and its aqueous solution is acidic and has reducing properties. Vitamin C widely exists in fruits and vegetables, and is one of the important indicators for the quality inspection of fruits and vegetables. Vitamin C is not only a nutrient required by the human body, but also a medical drug, and an antioxidant commonly used in the food industry. Vitamin C cannot be synthesized in the human body, and the vitamin C needed by the human body is mainly taken from fresh vegetables and fruits. Accurate determination of vitamin C content in food and medicine is of great significance to people's production a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/16G01N21/79
CPCG01N31/162G01N21/79
Inventor 翟丽萍贾秀峰郭宏文李波徐婷婷
Owner QIQIHAR UNIVERSITY
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