Meat-duck OTXR gene single nucleotide polymorphism and detecting method and application thereof

Abstract

The invention discloses meat-duck OTXR gene single nucleotide polymorphism and a detecting method and application thereof, and belongs to the field of molecular genetics detecting. The detecting process of the gene single nucleotide polymorphism includes the steps that to-be-detected meat-duct whole genome DNA containing OTXR genes serves as a template, a primer pair P serves as a primer, and the meat-duck OTXR genes are subjected to PCR amplification; after the PCR amplification product is digested through restriction enzymes PstI, amplified fragments obtained after digestion are subjected to agarose gel electrophoresis; the polymorphism of basic groups of the 946th nucleotide of second exons of the meat-duck OTXR genes is identified according to the electrophoresis result. According to the meat-duck OTXR gene single nucleotide polymorphism and the detecting method and application thereof, molecular genetic markers closely related to economic characters of meat ducks are screened and detected in the DNA level, and molecular bases are provided for rapid breeding of meat ducks.

Description

Technical field [0001] The present invention belongs to the field of molecular genetics, and relates to the use of single nucleotide polymorphisms (SNPs) of functional genes of meat ducks as molecular genetic markers, and in particular to the single nucleotide polymorphisms of meat duck OTXR genes and detection methods thereof and application. Background technique [0002] Single nucleotide polymorphism (SNP) refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence. Therefore, the commonly referred to as SNP includes base substitutions, insertions, deletions, and changes in the copy number of repeated sequences. A SNP means that there is a nucleotide change at a certain site in the genome, which is mainly caused by the conversion or transversion of a single base; SNPs with conversion variants account for about 2 / 3, and other SNPs are similar in Level. The cytosine of CpG dinucleotide is the most prone to mutation in the...

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Problems solved by technology

Due to the lack of research in the field of genetic variation of the OTXR gene in meat ducks, the functional research of this gene locus and the research on the relationship between genetic variation of this gene and economic traits have become blank

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