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Preparation method of lucid ganoderma protein peptide-selenium chelate

A chelate and protein peptide technology is applied in the field of preparation of Ganoderma lucidum protein peptide-selenium chelate, which can solve the problems of less research on Ganoderma lucidum protein and complex active ingredients, and achieves easy absorption, high extraction rate and low price. Effect

Inactive Publication Date: 2016-05-25
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The active ingredients contained in Ganoderma lucidum are complex, and the current research focuses on the biological activity of polysaccharides, and there are few studies on the protein of Ganoderma lucidum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1: Ultrafinely pulverize Ganoderma lucidum, mix the pulverized Ganoderma lucidum with distilled water at a w / v ratio of 1:40, use 1.0wt% NaOH to adjust the pH to 9.0, ultrasonic extraction for 10 minutes, ultrasonic power 100, water bath extraction 60min, temperature 60°C, after extraction, filter with gauze, the filtrate is the alkaline-extracted ganoderma protein solution, adjust the pH of the alkaline-extracted ganoderma protein solution to 3.0 with hydrochloric acid for protein precipitation, centrifuge at 4000r / min for 5min to obtain the precipitated protein, wash with water until neutral Centrifuge again, and freeze-dry the precipitate to obtain Ganoderma lucidum protein, with an extraction rate of 75.34%;

[0019] Step 2: Dissolve the ganoderma lucidum protein in water so that the protein concentration reaches 3.0wt%, adjust the pH of the ganoderma lucidum protein solution to 10.0 with NaOH solution, keep the temperature at 50°C, add 3.0wt% compound protease,...

Embodiment 2

[0027] Step 1: Ultrafinely pulverize Ganoderma lucidum, mix the pulverized Ganoderma lucidum with distilled water at a w / v ratio of 1:80, use 3.0wt% NaOH to adjust the pH to 10.0, ultrasonically extract for 50 minutes, ultrasonic power is 200W, and extract in a water bath 120min, temperature 100°C, after extraction, filter with gauze, the filtrate is the alkaline-extracted ganoderma protein solution, use hydrochloric acid to adjust the pH of the alkaline-extracted ganoderma protein solution to 2.5 for protein precipitation, centrifuge to obtain the precipitated protein, wash with water until neutral, centrifuge again, and Ganoderma lucidum protein was obtained by precipitation freeze-drying, and the extraction rate reached 82.75%;

[0028] Step 2: Dissolve Ganoderma lucidum protein in water so that the protein concentration reaches 3.0wt%, adjust the pH to 11.0, keep the temperature at 60°C, add 3.0wt% protease, hydrolyze for 60min, inactivate the enzyme, and obtain the Ganoder...

Embodiment 3

[0037] Step 1: Ultrafinely pulverize Ganoderma lucidum, mix the pulverized Ganoderma lucidum with distilled water at a w / v ratio of 1:60, gradually add solid ammonium sulfate to make the saturation of ammonium sulfate reach 50wt%, centrifuge at 5000r / min for 5min The Ganoderma lucidum protein was precipitated, desalted by dialysis and freeze-dried to obtain the Ganoderma lucidum protein, and the extraction rate reached 81.15%;

[0038] Step 2: Dissolve Ganoderma lucidum protein in water so that the protein concentration reaches 5.0wt%, adjust the pH to 8.0, keep the temperature at 40°C, add 5.0wt% neutral protease, hydrolyze for 50min, inactivate the enzyme, and obtain the Ganoderma lucidum protein polypeptide complex solution;

[0039] The third step: freeze-dry the obtained ganoderma protein polypeptide complex solution to make ganoderma protein polypeptide complex lyophilized powder, then mix sodium selenite powder and ganoderma protein polypeptide complex lyophilized powde...

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Abstract

The invention discloses a preparation method of a lucid ganoderma protein peptide-selenium chelate. The preparation method comprises the following steps: extracting protein in lucid ganoderma by virtue of an alkali dissolution acid settlement method or an ammonium sulfate settlement method; after carrying out restriction enzymolysis on the lucid ganoderma protein by virtue of alkaline protease, neutral protease or compound protease, and carrying out enzyme deactivation, so as to prepare lucid ganoderma protein proteolysis; and chelating selenium in an inorganic substance sodium selenite with lucid ganoderma protein peptide, so as to obtain the lucid ganoderma protein peptide-selenium chelate. By virtue of the preparation method, the extraction rate of the lucid ganoderma protein is remarkably increased and reaches 75.34%-82.75%; a mineral element-peptide chelate prepared by virtue of the preparation method has specific chelation system and transport mechanism and is easy to absorb, safe, non-toxic, low in cost and capable of supplementing amino acids and mineral ions; and the selenium content of the prepared lucid ganoderma protein peptide-selenium chelate is 2326ug / g-4048ug / g, and thus a theoretical basis and a technical support are provided for the development of organic selenium nutrition supplements and functional foods.

Description

technical field [0001] The invention relates to a preparation method of ganoderma lucidum protein peptide-selenium chelate, which belongs to the field of food biotechnology. Background technique [0002] Selenium (Se) is an important mineral element necessary for the human body and has important physiological functions. A large number of existing studies have shown that many diseases are closely related to the nutritional status of Se. The human body mainly obtains selenium through diet, and the method of artificial selenium supplementation is oral selenium-containing preparations. The general method of supplementing selenium is to make oral preparations with inorganic selenium compounds (sodium selenite and sodium selenate). However, the effect of absorption and utilization of inorganic selenium enhancers is not ideal, the bioavailability is low, and it has strong toxic and side effects. , so its use is strictly limited. Studies have shown that organic selenium compounds...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/30
CPCC07K1/30C12P21/06
Inventor 赵立娜刘斌陈紫红陈濠范锦琳林占熺吕旭聪刘晓艳
Owner FUJIAN AGRI & FORESTRY UNIV
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