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Fermentation extract of marine fungus Trichoderma harzianum DLEN2008005 and preparation method and application of fermentation extract

A technology of DLEN2008005, marine fungi, applied in the field of microorganisms, can solve problems such as brain cell aging, death, and brain tissue damage

Inactive Publication Date: 2016-05-25
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, many studies have found that superoxide free radicals produced in the process of aerobic cell metabolism can cause damage to brain tissue, promote the aging and death of brain cells, and free radicals can also damage cell chromosomes, causing chromosome 21 distortion, thus AD occurs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Isolation and identification of marine fungus Trichoderma harzianum DLEN2008005

[0057] 1. Separation method

[0058] (1) Sample: Asparagus algae from intertidal seaweed in Dalian sea area, China.

[0059] (2) Separation method

[0060] Rinse the above-mentioned fresh seaweed sample twice with seawater sterilized at 121°C for 20 minutes, transfer it into a 200mL Erlenmeyer flask, add glass beads and 50mL of sterile seawater after autoclaving at 121°C for 20 minutes, and oscillate at 60rpm After 10 minutes, the supernatant was discarded, and then washed twice with sterile seawater algae.

[0061] Soak the above-mentioned seaweed in 50mL of 70% ethanol aqueous solution for 10 seconds, take it out, rinse with 50mL of sterile seawater to remove residual ethanol on the surface, blot the remaining seawater with sterile absorbent paper, and burn the seaweed with a sterilized blade Obliquely cut into small pieces, and transplanted to a flat solid medium for 3 days...

Embodiment 2

[0076] Example 2 Activity test of marine fungus Trichoderma harzianum DLEN2008005 fermentation broth

[0077] 1. Obtained from Trichoderma harzianum DLEN2008005 fermentation broth

[0078] (1) Seed culture: inoculate the bacteria on a sterile seed solid medium plate from a slant, place the culture plate in a constant temperature incubator at 28°C for upside-down culture, and the culture time is 4 to 5 days;

[0079] (2) Expansion culture: fill 1L of liquid medium into a 3L Erlenmeyer flask, seal it with a gas-permeable sealing film, and then sterilize in an autoclave at 121°C and 0.1Mpa for 20 minutes; after cooling, inoculate the above-mentioned Step (1) After cultivating, the culture medium block of the seed plate is 40-80cm 2 The proportion of inoculation, sealing, placed in a constant temperature incubator culture, natural light, culture time is 21 to 24 days, to obtain fermentation liquid.

[0080] Among them, the medium used:

[0081] The seed solid medium is: 20.0g s...

Embodiment 3

[0085] Example 3 Extraction of Active Substances from Marine Fungus Trichoderma harzianum DLEN2008005 Fermentation Broth

[0086] 1. Medium

[0087] (1) Seed solid medium: 20.0g sucrose, 500mL potato juice, 20.0g sea salt, 15.0g agar, natural pH.

[0088] (2) Liquid medium: 20.0g sucrose, 500mL potato juice, 20.0g sea salt, natural pH.

[0089] Among them, potato juice: take fresh potatoes, peeled and cut into 1cm 3 For every 200g of potato pieces, add 500mL of distilled water to boil for 20min, filter through double-layered gauze, and distill the volume to 500mL with distilled water.

[0090] 2. Extraction method

[0091] (1) Seed culture: inoculate the bacteria on a sterile seed solid medium plate from a slant, place the culture plate in a constant temperature incubator at 28°C for upside-down culture, and the culture time is 4 to 5 days;

[0092] (2) Expansion culture: fill 1L of liquid medium into a 3L Erlenmeyer flask, seal it with a gas-permeable sealing film, and th...

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PUM

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Abstract

The invention discloses a marine fungus Trichoderma harzianum DLEN2008005 and application thereof. The marine fungus Trichoderma harzianum DLEN2008005 is collected in China General Microbiological Culture Collection Center (CGMCC) on January 29, 2016, the collection number is CGMCC No.12105, and the collection address is Institute of Microbiology of Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing City. Meanwhile, active substances in standing fermentation liquid of the marine fungus Trichoderma harzianum DLEN2008005 are extracted and purified, and the strain, a culture of the strain and an extract of the fermentation liquid have very good antioxidant activity and acetylcholin esterase inhibition activity and have very good application prospects in preparation of anti-senile dementia medicines, antioxidant medicines and / or medicines for inhibiting the activity of acetylcholin esterase.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a fermented extract of the marine fungus Trichoderma harzianum DLEN2008005 and its preparation method and application. Background technique [0002] With the aging of the world's population and the increasing incidence of Alzheimer's Disease (AD), it is imminent to find new drugs against Alzheimer's disease. [0003] Currently, the widely accepted pathological theory of Alzheimer's is the cholinergic nerve damage hypothesis. Under the guidance of this theory, acetylcholinesterase (AChE) inhibitors are currently the most widely used anti-Alzheimer's drugs. At the same time, many studies have found that superoxide free radicals produced in the process of aerobic cell metabolism can cause damage to brain tissue, promote the aging and death of brain cells, and free radicals can also damage cell chromosomes, causing chromosome 21 distortion, thus AD occurs. ...

Claims

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Application Information

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IPC IPC(8): C12P1/02A61K36/06A61P25/28C12R1/885
CPCA61K36/06C12P1/02C12N1/145C12R2001/885
Inventor 张翼鲍海燕聂影影黎燕媚冯妍刘敬珊黎钊坪许茂鑫宋采胡雪琼
Owner GUANGDONG OCEAN UNIVERSITY
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