Preparing method for co-immobilized enzyme for accelerating aging and clarifying Shanxi aged vinegar
A technology of co-immobilizing enzymes and mature vinegar, applied in the preparation of vinegar, immobilizing on/in organic carriers and other directions, can solve the problems of difficult separation and recovery, denaturation of enzyme proteins, poor stability, etc., to improve the enzymology properties, overcoming poor stability and improving light transmittance
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Embodiment 1
[0034] Prepare co-immobilized enzyme with the method of the present invention, comprise the steps:
[0035] (1) prepare acetic acid-sodium acetate buffer solution of pH3.6, pH4.6 acetic acid-sodium acetate buffer solution, 2% calcium chloride aqueous solution;
[0036] (2) Prepare the carrier material with a mass ratio of sodium alginate and xanthan gum of 5:1 to prepare the carrier material;
[0037] (3) Preparation of esterase enzyme liquid Accurately weigh a certain amount of esterase enzyme powder, dissolve it with acetic acid-sodium acetate buffer solution with 4 times the esterase mass and pH 3.6, and pour the gained supernatant into volumetric flask, add acetic acid-sodium acetate buffer solution of pH 3.6 to the remaining sediment and grind for several times until it is completely dissolved, then transfer it into the volumetric flask, then dilute it with buffer solution to the scale of the volumetric flask, and shake it well for more Layer gauze is filtered, is prepar...
Embodiment 2
[0046] Prepare co-immobilized enzyme with the method of the present invention, comprise the steps:
[0047] (1) prepare acetic acid-sodium acetate buffer solution of pH3.6, pH4.6 acetic acid-sodium acetate buffer solution, 2% calcium chloride aqueous solution;
[0048] (2) Prepare the carrier material with a mass ratio of sodium alginate and xanthan gum of 5:1 to prepare the carrier material;
[0049] (3) Preparation of esterase enzyme liquid Accurately weigh a certain amount of esterase enzyme powder, dissolve it with acetic acid-sodium acetate buffer solution with 5 times the esterase mass and pH 3.6, and pour the gained supernatant into volumetric flask, add acetic acid-sodium acetate buffer solution of pH 3.6 to the remaining sediment, mash it for several times until it is completely dissolved, then transfer it into the volumetric flask, then dilute it with buffer solution to the scale of the volumetric flask, and shake it well for more Layer gauze is filtered, is prepare...
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