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Solid fermentation method for cordyceps sinensis mycelia

A technology of Cordyceps sinensis mycelium and solid fermentation, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., and can solve the problems of high price of wild Cordyceps sinensis and high cost of Cordyceps sinensis mycelium powder.

Inactive Publication Date: 2016-05-18
李光辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a simple, effective and low-cost preparation method suitable for industrial production in view of the problems that wild Cordyceps is expensive and the cost of producing Cordyceps mycelium powder by liquid fermentation is relatively high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Bacteria culture

[0032] 1. Strain: the strain is Hirsutella inensis purchased from the Institute of Microbiology, Chinese Academy of Sciences.

[0033] 2. Liquid primary strain culture:

[0034] Primary culture medium: potato powder 15g; glucose 15g; MgSO 4 ·7H 2 O0.2g; KH 2 PO 4 0.5g; peptone 3g; yeast extract 4g; purified water to volume 1000ml. Packed in eight 1L Erlenmeyer flasks. Sterilization: 121°C, 30 minutes.

[0035] Cut the strain into 5mm 2 For the left and right small pieces, add 2 pieces to the 1L Erlenmeyer flask filled with the above-mentioned primary strain liquid medium, and cultivate them in a full-temperature shaking incubator with a rotation speed of 150r / m at a temperature of 16°C for 10 days.

[0036] 3. Liquid secondary strain culture:

[0037] Secondary strain medium: potato powder 315g; glucose 315g; MgSO 4 ·7H 2 O4.2g; KH 2 PO 4 10.5g; peptone 63g; yeast extract 84g; CaCO 3 Powder 10.5g; the rest is pure water, 21L in total....

Embodiment 2

[0048] 1. Bacteria culture

[0049] 1. Strain: the strain is Hirsutella inensis purchased from the Institute of Microbiology, Chinese Academy of Sciences.

[0050] 2. Liquid primary strain culture:

[0051] Primary culture medium: potato powder 30g; glucose 25g; MgSO 4 ·7H 2 O0.5g; KH 2 PO 4 1.0g; peptone 5g; yeast extract 6g; purified water to volume 1000ml. Divide into 8 1L Erlenmeyer flasks, and sterilize at 121°C for 30 minutes.

[0052] Cut the strain into 5mm 2 The left and right pieces were added to 3 pieces in a 1L Erlenmeyer flask filled with the above-mentioned liquid medium, and cultured in a full-temperature shaking incubator with a rotation speed of 180r / m at a temperature of 18°C ​​for 6 days.

[0053] 3. Liquid secondary strain culture:

[0054] Secondary strain medium: potato powder 720g; glucose 600g; MgSO 4 ·7H 2 O12g; KH 2 PO 4 24g; peptone 120g; yeast extract 144g; CaCO 3 Powder 24g; the rest is purified water, a total of 24L.

[0055] Put 2.4...

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Abstract

The invention discloses a solid fermentation method for cordyceps sinensis mycelia. The method comprises the steps of strain culture and solid fermentation culture, wherein Hirsutella sinensis is adopted as a strain, and a solid medium is inoculated with the strain for fermenting production. Effective ingredients of the product prepared according to the solid fermentation method are consistent with or approximate to those of wild cordyceps sinensis; besides, cost is low, process control is simple, pollution is avoided, and safety and effectiveness are achieved.

Description

technical field [0001] The invention belongs to the field of fungal biological fermentation and relates to a solid fermentation method of Cordyceps sinensis mycelium. Background technique [0002] Cordyceps sinensis (Berk.) Sacc. is a famous traditional Chinese medicine in my country. It is as famous as ginseng and velvet antler. The three are collectively called "three treasures of traditional Chinese medicine". Cordyceps sinensis contains many effective substances such as cordycepin, cordyceps polysaccharide, adenosine, and cordycepic acid. It has the functions of nourishing the kidney and lung, stopping bleeding and reducing phlegm, coughing, anti-cancer, improving immunity, and anti-fatigue. Because the output of wild Cordyceps sinensis has been decreasing year by year, the market price has been rising continuously in recent years, which is called "soft gold". [0003] In order to solve the current situation that the market is in short supply, the artificial cultivation...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 李光辉
Owner 李光辉
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