Method for assaying amphetamines, ketamine, pethidine and methadone in blood and urine

Abstract

The invention belongs to the technical field of analytical chemistry, and relates to a method for rapidly assaying four amphetamines (amphetamine, methamphetamine, methylene-dioxy-amphetamine and methylene-dioxy-methamphetamine), ketamine, pethidine and methadone in blood and urine. The method is high in sensitivity and specificity and wide in linear range, can be used for qualitatively and quantitatively assaying analytes of unknown concentrations in the blood and the urine, and is simple and rapid to operate; according to the method, processes of extracting, and drying by evaporating are not required, and characteristics that a judicial expertise task is urgent and has a high requirement on assaying time can be met; an assaying system, provided by the invention, provides a new technical platform for drug assaying, and is easy to popularize and apply.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, in particular to a method for measuring amphetamines, ketamine, pethidine and methadone in blood and urine. This method can be used to quickly and easily determine 4 kinds of amphetamines (amphetamine, methamphetamine, methylenedioxyamphetamine, methylenedioxymethamphetamine) ketamine, pethidine and methadone and other compounds in blood and urine, especially suitable for in the field of forensic identification. Background technique [0002] According to data, currently widely abused drugs include four amphetamine compounds (amphetamine (AMP), methamphetamine (MAMP), methylenedioxyamphetamine (methylenedioxyamphetamine, MDA), methylenedioxy Methamphetamine (methylenedioxymethamphetamine, MDMA), as well as ketamine and pethidine. [0003] Studies have shown that the toxicity of AMP and MAMP is mainly based on the excitatory effect of the central nervous system; MDA and MDMA have both excitato...

AI Technical Summary

Problems solved by technology

Practice shows that the specificity of the enzyme-linked immunosorbent assay is poor, it is difficult to distinguish structural analogs, and false positives often occur. Therefore, it can only be used as a means of preliminary screening, and further gas chromatography-mass spectrometry or Liquid chromatography-mass spectrometry is used for confirmation, and before biological samples are analyzed by gas chromatography-mass spectrometry or liquid chromatography-mass spectrometry, the samples need to be extracted and purified, and this step involves extracting the liquid The process of evaporating to dry, so it often occurs and studies have shown that AMP and MAMP quickly volatilize with the evaporating of the extract, which affects the reproducibility of the analysis and the linearity of the standard curve, making it difficult for the substance to be tested in the biological sample. Accurate Quantification of Defects

Some researchers try to avoid the volatilization of AMP and MAMP by adding acid or using low temperature conditions to evaporate the sample or through derivatization. However, the method used is cumbersome and time-consuming, and it is still difficult to meet the requirements of forensic identification for rapid drug analysis.

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