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Method for preparing anhydrous chlorhematin by utilizing yak blood

A technology of hemin and yak is applied in the preparation of anhydrous hemin, and the field of preparing anhydrous hemin by using yak blood can solve the problems of environmental pollution, production cost, waste of reagents, etc., and achieve less chemical reagents. , the method is simple, the effect of convenient industrial production

Inactive Publication Date: 2016-05-11
兰州天禾生物催化技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are many methods for preparing hemin, but basically a large amount of chemical reagents are used, which not only causes waste of reagents and environmental pollution, but also makes the production cost very high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1. Collection of red blood cells: Use a 50ml test tube to collect 20ml of fresh yak blood, filter it with gauze to remove larger impurities, collect the filtrate, let it stand for 1 hour and wait for the blood to coagulate and stratify, then suck off the light yellow liquid on the upper layer. Obtain 12ml of red blood cell coagulum in the lower layer.

[0018] 2. Red blood cell crushing: Add 6ml of deionized water to the red blood cell coagulum, put it into the ultrasonic wave for 15 minutes, and stir it with a glass rod at the same time to fully disperse the solid coagulum, and at the same time break the red blood cell and release hemoglobin. Quickly pour the solution into six small test tubes, heat at 60°C for 30 minutes in the dark, then centrifuge for 10 minutes with a 3000r / min centrifuge, pour the upper layer liquid in the six small test tubes into a 20ml Erlenmeyer flask, Remove the denatured impurities in the lower layer to obtain hemoglobin solution.

[0019] ...

Embodiment 2

[0022] 1. Collection of erythrocytes: with embodiment 1.

[0023] 2. Fragmentation of red blood cells: add 12 ml of deionized water to the coagulum of red blood cells, the remainder is the same as in Example 1.

[0024] 3 Enzymatic hydrolysis of hemoglobin: adjust the collected hemoglobin solution to PH=7.0 with HCl0.1mol / L standard solution, put it in a constant temperature water bath at 50°C and heat it for 20min, add 0.3g of neutral protease with an enzyme activity of 4000u / g, The temperature is 45°C; after hydrolysis for 5 hours, add 0.5 g of flavor enzyme with an enzyme activity of 2000 u / g, adjust to pH = 6.9, and the rest are the same as in Example 1.

[0025] 4. The extraction of hemin: with embodiment 1.

Embodiment 3

[0027] 1. Collection of erythrocytes: with embodiment 1.

[0028] 2. Broken erythrocytes: add 15ml of deionized water to the coagulum of erythrocytes, the remainder is the same as in Example 1.

[0029] 3 Enzymatic hydrolysis of hemoglobin: adjust the collected hemoglobin solution to PH=7.0 with HCl0.1mol / L standard solution, put it in a constant temperature water bath at 50°C and heat it for 20min, add 0.3g of neutral protease with an enzyme activity of 4000u / g, The temperature is 45°C; after hydrolysis for 5 hours, add 0.5 g of flavor enzyme with an enzyme activity of 2000 u / g, adjust to pH = 7.0, and the rest are the same as in Example 1.

[0030] 4. The extraction of hemin: with embodiment 1.

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Abstract

The invention belongs to the technical field of biology, and relates to a method for preparing anhydrous chlorhematin by utilizing yak blood. The method is characterized in that the method is carried out according to the steps of collection of erythrocyte, crushing of the erythrocyte, enzymolysis of hemoglobin and extraction of chlorhematin. According to the method disclosed by the invention, the anhydrous chlorhematin is prepared by utilizing the yak blood, so that an animal blood resource is fully and valuably utilized; the method for preparing the anhydrous chlorhematin is simple and is easy to operate, used chemical reagents are less, the cost is lower, the environment pollution is lower, and the method is convenient for industrial production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for preparing anhydrous hemin, in particular to a method for preparing anhydrous hemin by using yak blood. Background technique [0002] Yak is one of the main breeds of cattle in China. It is the dominant and leading livestock species in the pastoral areas of the Qinghai-Tibet Plateau, with tenacious vitality. my country is the birthplace of the world's yaks. 90% of the world's yaks live on the Qinghai-Tibet Plateau and the six adjacent provinces. The protein of yak meat is as high as 21%, low in fat, full of amino acid varieties, good in quality and good in flavor. Yak milk is thick, with a dry matter content of about 18%, a fat content of about 7%, and a protein content of about 5%. Fur has characteristics and has great development value. According to calculations, the comprehensive development of yak meat, milk, fur, skin, bone, blood, etc. can obtain an economic added ...

Claims

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Application Information

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IPC IPC(8): C12P17/18
CPCC12P17/182
Inventor 高维东谢小冬弥超魏美玲朱明
Owner 兰州天禾生物催化技术有限公司
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