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Probiotics microcapsule preparation and preparation method thereof

A technology of microcapsules and probiotics, applied in the biological field, can solve the problems of no spore formation, large loss of effective components, and difficulty in long-term storage, and achieve the effects of preventing the destruction of gastric juice, preventing the inactivation of bacterial cells, and improving stability.

Active Publication Date: 2016-05-11
魏永刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Enterococcus faecium and Lactobacillus casei do not form spores during the growth process, so they have poor stress resistance and are difficult to store for a long time in liquid conditions
The loss of the active ingredients of the product is relatively large, and the shelf life of the product is also short, which limits its large-scale application

Method used

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  • Probiotics microcapsule preparation and preparation method thereof
  • Probiotics microcapsule preparation and preparation method thereof
  • Probiotics microcapsule preparation and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0032] Such as figure 1 and figure 2 As shown, the present invention provides a probiotic microcapsule preparation, which includes a capsule material and a wall material. The capsule material contains probiotics, and the probiotics are Enterococcus faecium and Lactobacillus casei.

[0033] A kind of preparation method of probiotic microcapsule preparation, comprises the following steps:

[0034] (1) Cultivation of seed liquid: Enterococcus faecium and Lactobacillus casei are respectively cultured in liquid medium to independently cultivate Enterococcus faecium to the end of logarithmic growth, and become Enterococcus faecium at the end of logarithmic growth; independently cultivate Lactobacillus casei to At the end of logarithmic growth, Lactobacillus casei becomes Lactobacillus casei at the end of logarithmic growth; this method requires Enterococcus faecium and Lactobacillus casei seed liquid to be cultured independently to ensure that they have the best proliferation acti...

Embodiment 2

[0048] A probiotic microcapsule preparation comprises a capsule material and a wall material. The capsule material contains probiotics, and the probiotics are Enterococcus faecium and Lactobacillus casei.

[0049] A kind of preparation method of probiotic microcapsule preparation, comprises the following steps:

[0050] (1) Cultivation of seed liquid: Enterococcus faecium and Lactobacillus casei were separately cultured in MRS liquid medium, and Enterococcus faecium was independently cultivated to the end of logarithmic growth, becoming Enterococcus faecium at the end of logarithmic growth; Lactobacillus casei was independently cultivated At the end of logarithmic growth, Lactobacillus casei becomes Lactobacillus casei at the end of logarithmic growth; this method requires Enterococcus faecium and Lactobacillus casei seed liquid to be cultured independently to ensure that they have the best proliferation activity.

[0051] (2) Preparation of microcapsule capsule material solut...

Embodiment 3

[0057] A probiotic microcapsule preparation comprises a capsule material and a wall material. The capsule material contains probiotics, and the probiotics are Enterococcus faecium and Lactobacillus casei.

[0058] A kind of preparation method of probiotic microcapsule preparation, comprises the following steps:

[0059] (1) Cultivation of seed liquid: Enterococcus faecium and Lactobacillus casei were separately cultured in MRS liquid medium, and Enterococcus faecium was independently cultivated to the end of logarithmic growth, becoming Enterococcus faecium at the end of logarithmic growth; Lactobacillus casei was independently cultivated At the end of logarithmic growth, Lactobacillus casei becomes Lactobacillus casei at the end of logarithmic growth; this method requires Enterococcus faecium and Lactobacillus casei seed liquid to be cultured independently to ensure that they have the best proliferation activity.

[0060] (2) Preparation of microcapsule capsule material solut...

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Abstract

The invention relates to a probiotics microcapsule preparation and a preparation method thereof. The microcapsule preparation comprises a capsule material and a wall material. The wall material is composed of soybean oil and Span 80. The capsule material contains enterococcus faecium and lactobacillus casei. Before the fermentation, the microbes are embedded in the microcapsules. Low density microbial cells are wrapped in the capsule material, and then fermentation is carried out to obtain higher bacterium concentration and cell activity. Through simple separation, a dual lactobacillus microcapsule preparation containing high density enterococcus faecium and lactobacillus casei can be obtained directly. The preparation method has the advantages that the microbes are embedded before fermentation, two different lactobacillus cells are embedded into a same microcapsule at the same time, the intergrowth of different microbial cells is realized, the synergetic effect can be generated; due to the pre-fermentation embedding technology, two microbial cells can proliferate for another time, and thus higher activities can be obtained more easily.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a probiotic microcapsule preparation and a preparation method thereof. Background technique [0002] Enterococcus faecium, as an indigenous flora in the intestinal tract of livestock and poultry, belongs to the lactic acid bacteria group. Enterococcus faecium plays an important role in maintaining the ecological balance of animal intestinal flora, especially in the intestinal health care of young animals and the prevention and treatment of diseases. [0003] Lactobacillus casei, as a lactic acid bacteria probiotic, has been widely used in the fields of medicine and food engineering. Lactobacillus casei has the functions of effectively lowering blood pressure and cholesterol, producing antibody immunity, and enhancing human immunity. [0004] Enterococcus faecium and Lactobacillus casei do not form spores during the growth process, have poor stress resistance, and are di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/10C12N1/20A61K35/744A61K35/747A61K9/50A61P1/00A61P9/12A61P3/06A61P37/04C12R1/245
CPCA61K9/5031A61K35/744A61K35/747C12N1/20C12N11/10C12N11/14A61K2300/00
Inventor 魏永刚栾兆文
Owner 魏永刚
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