Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Amphiprotic lanthanum carboxylate metal organic framework and synthesis method thereof, and application of amphiprotic lanthanum carboxylate metal organic framework

A metal-organic framework, amphoteric carboxylic acid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as application research limitations, MOFs framework collapse, etc.

Inactive Publication Date: 2016-05-11
SOUTHERN MEDICAL UNIVERSITY
View PDF1 Cites 16 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although the prospect of MOFs materials for detection of viral nucleic acid molecules has attracted widespread attention from scientists, the few MOFs reported in the literature for the detection of viral nucleic acid molecules are all compounds with stable properties to water screened from the literature. , a large number of synthesized MOFs are sensitive to water and lead to framework collapse, which greatly limits their further application research.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Amphiprotic lanthanum carboxylate metal organic framework and synthesis method thereof, and application of amphiprotic lanthanum carboxylate metal organic framework
  • Amphiprotic lanthanum carboxylate metal organic framework and synthesis method thereof, and application of amphiprotic lanthanum carboxylate metal organic framework
  • Amphiprotic lanthanum carboxylate metal organic framework and synthesis method thereof, and application of amphiprotic lanthanum carboxylate metal organic framework

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of amphoteric lanthanum carboxylate metal organic framework, its chemical formula is {[La 4 (Cmdcp) 6 (H 2 O) 9 ]} n (1) (n represents the degree of aggregation, which is a natural number).

Embodiment 2

[0029] The preparation reaction formula of complex of the present invention is as follows:

[0030]

[0031] Specific steps are as follows:

[0032] a. Weigh the solid H according to the amount of the substance 3 CmdcpBr (91.5 mg, 0.3 mmol) was suspended in MeOH (5 mL) solution, and the pH was adjusted to 7.0 with 0.1 mM NaOH solution to obtain a clear solution.

[0033] b. Weigh the solid La(NO 3 ) 3 ·6H 2 O (86.6 mg, 0.2 mmol) was dissolved in MeOH solution (5 mL) to give a clear solution of the lanthanum salt.

[0034] c, the molar ratio of 2:3 La(NO 3 ) 3 ·6H 2 O solution was added dropwise to H 3 In the clear solution of CmdcpBr, the reaction was stirred at room temperature for 0.5h, and a large amount of white precipitates appeared in the reaction.

[0035] d, the precipitate is filtered, and the precipitate is washed with MeOH (5mL), and the white precipitate is washed with H 2 O (100 mL) was dissolved at 100 °C and filtered to obtain a clear solution.

[...

Embodiment 3

[0052] Compound {[La 4 (Cmdcp) 6 (H 2 O) 9 ]} n (1) Detect HIVds-DNA and SUDVRNA experiment (obtain compound in embodiment 1)

[0053] (1) Preparation of compound 1 solution

[0054] Compound 1 was dissolved in distilled water to prepare a solution with a concentration of 0.1mM, sealed and stored at room temperature; 2 ) to dissolve DNA and RNA to a concentration of 0.1 mM, seal and store in a refrigerator.

[0055] (2) Detection of HIVds-DNA and SUDVRNA experiments

[0056] Dilute the probe DNA solution with a buffer solution to a concentration of 50nM, take 2mL of the solution and put it into a cuvette with a capacity of 4mL. After adjusting the parameters of the fluorometer, test the initial fluorescence intensity of the solution. A certain amount of compound solution was added to the solution, and after stirring for a period of time, the fluorescence intensity of the mixed solution was tested. The compound was continuously added and tested until the fluorescence in...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an amphiprotic lanthanum carboxylate metal organic framework and a synthesis method thereof, and application of the amphiprotic lanthanum carboxylate metal organic framework. The chemical formula is {[La4(Cmdcp)6(H2O)9]}n (1), wherein n represents polymerization degree and is a natural number. The amphiprotic lanthanum carboxylate metal organic framework can be used for detecting the HIV (human immunodeficiency virus) DNA or SUDV (Sudan ebolavirus) RNA nucleic acid sequence. The product has the advantages of wide raw material sources and accessible raw materials. The synthesis method of the product has the advantages of mild reaction conditions and short time. The product can be obtained by recrystallization in a water solution, is nontoxic and harmless, and thus, implements green chemistry. The synthesis method does not need any inert gas protective atmosphere. The yield is 86% or above, and the purity is up to 100%. The product has the following advantages in the aspect of activities: the product simultaneously has effective detection effects on viruses HIV ds-DNA and SUDV RNA; and the product has the advantages of quick and efficient detection and high sensitivity, and is simple to operate. The product is hopeful to become a novel in-vitro detection reagent for virus nucleic acid sequences.

Description

technical field [0001] The invention relates to the fields of coordination chemistry and inorganic medicinal chemistry, in particular to an amphoteric lanthanum carboxylate metal-organic framework and its synthesis method and application. Background technique [0002] As we all know, diseases such as AIDS and Ebola hemorrhagic fever caused by viruses are incurable, easily contagious, and currently there is no effective preventive vaccine or specific treatment drug. Therefore, it is imperative to find early diagnostic reagents and isolate and treat them to reduce the risk of virus transmission. [0003] The current clinical methods for detecting viral nucleic acid sequences (such as PCR, Southern blot) generally have defects such as cumbersome operation, time-consuming, high cost, and prone to false positive results. Therefore, finding an effective method with simple preparation, easy operation, high selectivity, and rapid detection of toxic nucleic acid molecular sequences ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C08G83/00C12Q1/70C12Q1/68
CPCC08G83/008C12Q1/6818C12Q2563/107
Inventor 陈金香杨水平孙斌
Owner SOUTHERN MEDICAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com