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Lignum aquilariae resinatum extract, pharmaceutical composition with same and application thereof

A technology of agarwood extract and composition, which is applied in the field of preparation of medicines for diseases caused by disorders of glucose and lipid metabolism, and can solve the literature reports that no agarwood extract and no effect of agarwood extract on improving glucose and lipid metabolism disorders, There are no problems such as the insulin-stimulating effect of the agarwood extract, and the effect of improving the injection glucose tolerance, improving the impaired glucose tolerance, and improving angina pectoris is achieved.

Active Publication Date: 2016-05-11
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] So far, there are no studies on the effect of Agarwood extract (KDD) on lowering blood sugar, blood fat, anti-atherosclerosis and improving fatty liver, and there is no literature report on the effect of Agarwood extract on improving glucose and lipid metabolism disorders; Literature Reports on Insulin-stimulating Effect of Agarwood Extract

Method used

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  • Lignum aquilariae resinatum extract, pharmaceutical composition with same and application thereof
  • Lignum aquilariae resinatum extract, pharmaceutical composition with same and application thereof
  • Lignum aquilariae resinatum extract, pharmaceutical composition with same and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Embodiment 1: Preparation of Agarwood extract of the present invention

[0051] (1) After pulverizing 500 g of agarwood, reflux extraction with 4 L of 95% by volume ethanol aqueous solution for 2 hours to obtain an extract; repeat the extraction twice in the same way, and combine the extracts.

[0052] (2) Concentrate and dry the extract obtained in step (1) under reduced pressure to obtain 93g of extract; dissolve the extract with 500ml of water, extract 3 times with petroleum ether (1000ml / time), and combine the extracts 3 times to obtain petroleum ether extract; and then extract the aqueous layer with ethyl acetate 3 times (1000ml / time), and combine the three extracts to obtain the ethyl acetate extract.

[0053] (3) The ethyl acetate extract obtained in step (2) was concentrated and dried under reduced pressure to obtain 46 g of ethyl acetate extract.

Embodiment 2

[0054] Example 2: Experimental Research on Insulin Secretion Stimulation of Agarwood Extract of the Present Invention

[0055] 1. Experimental materials:

[0056] 1. Drugs: (1) Test drug: Agarwood extract of the present invention; (2) Positive control drug: Atorvastatin (Pfizer Pharmaceutical Co., Ltd., H20051407), Gliclazide (Tianjin Huajin Pharmaceutical Co., Ltd. , H10910053).

[0057] 2. Animals: SPF grade C57BL / 6ApoE- / - mice, 6-8 weeks old, weighing 19-23g, male; provided by the Animal Center of Peking University Health Science Center.

[0058] 3. Reagents and instruments: Glucose (Beijing Baierdi Biotechnology Co., Ltd.); Insulin injection (NovoNordisk, Denmark); Mouse insulin ELISA quantitative detection kit (Mercodia, Sweden); blood glucose meter and blood glucose test paper (Roche Vitality, GU type, Germany); multi-functional microplate reader (TECAN, Switzerland, M1000 type); centrifuge (Eppendorf, USA, 5424R type); electronic balance (Switzerland METTLERTOLEDO ...

Embodiment 3

[0090] Example 3: Experimental Research on Insulin Secretion Stimulation of Agarwood Extract of the Present Invention

[0091] 1. Experimental materials:

[0092] 1. Cells: Hamster HIT-T15 cells

[0093] 2. Test drug: Agarwood extract of the present invention

[0094] 3. Reagents and instruments: KRB buffer (Krebs-Ringer Bicarbonate Buffer); RPMI1640 cell culture medium and fetal bovine serum (Corning, USA); Gliclazide (China Institute for Food and Drug Control); cell culture box (SANYO, MCO -18AIC); ultra-clean bench (ESCO company, OptiMair); centrifuge (Eppendorf, 5424R type); multifunctional microplate reader (Switzerland TECAN company, M1000 type); mouse insulin ELISA quantitative detection kit (Sweden Mercodia company ).

[0095] 2. Experimental method:

[0096] HIT-T15 cells were cultured with RPMI1640 complete medium containing 10% (volume percentage) fetal bovine serum, and when they grew logarithmically, they were digested with 0.25% trypsin, and then resuspende...

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Abstract

The invention provides lignum aquilariae resinatum extract. The lignum aquilariae resinatum extract is characterized in that the lignum aquilariae resinatum extract is prepared by the aid of a method including steps of (1), smashing lignum aquilariae resinatum and extracting the lignum aquilariae resinatum by the aid of methanol or ethanol aqueous solution to obtain extract liquid; (2), concentrating and drying the extract liquid obtained in the step (1) under normal pressures or reduced pressures to obtain extract cream, or carrying out spray-drying on the extract liquid to obtain powder, then dissolving the extract cream or the powder in water to obtain solution, extracting the solution by the aid of petroleum ether to obtain petroleum ether extract liquid, and extracting aqueous layers by the aid of ethyl acetate to obtain ethyl acetate extract liquid; (3), concentrating and drying the ethyl acetate extract liquid obtained in the step (2) under reduced pressures to obtain extract cream, or carrying out spray-drying on the ethyl acetate extract liquid to obtain powder. The lignum aquilariae resinatum extract and the method have the advantage that the lignum aquilariae resinatum extract or a pharmaceutical composition with the lignum aquilariae resinatum extract can be used for preparing medicines for preventing and / or treating diseases due to glucose and lipid metabolism disorder.

Description

technical field [0001] The invention belongs to the technical field of pharmacy. Specifically, the present invention relates to the use of the plant extract extracted from the Agarwood plant in the preparation of medicines for diseases caused by glucose and lipid metabolism disorders. Background technique [0002] Insulin resistance (Insulin Resistance, IR) refers to the reduced responsiveness of the body to the physiological effects of insulin, which is manifested in the decreased sensitivity of peripheral tissues to insulin and the impairment of glucose utilization. The main reason is that due to the reduction of high-calorie diet or physical activity, as well as other aging factors, stress state, genetic factors, etc., the response of the target tissue of the body to insulin is weakened, and the ability of insulin to maintain blood sugar is reduced. Fat and protein and other substance metabolism disorders and energy metabolism disorders (Liu Liping. Establishment of insu...

Claims

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Application Information

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IPC IPC(8): A61K36/835A61P3/00A61P3/06A61P3/10A61P9/10A61P1/18A61P1/16A61P5/50
Inventor 屠鹏飞郑姣李军赵云芳刘炳琳伦琦星顾小盼
Owner BEIJING UNIV OF CHINESE MEDICINE
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